A5089334526- Prostate Cancer Treatment and Research
- Renal cell carcinoma treatment
- Cancer Immunotherapy and Biomarkers
- Bladder and Urothelial Cancer Treatments
- Prostate Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Testicular diseases and treatments
- Renal and related cancers
- Urinary and Genital Oncology Studies
- Cancer Genomics and Diagnostics
- Urological Disorders and Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Extracellular vesicles in disease
- Inflammatory Biomarkers in Disease Prognosis
- Immune cells in cancer
- Multiple and Secondary Primary Cancers
- Radiopharmaceutical Chemistry and Applications
- Sarcoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Urologic and reproductive health conditions
- Medical Imaging and Pathology Studies
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
Osaka University
2015-2024
Osaka International Cancer Institute
2020-2024
Suita Municipal Hospital
2018
Laboratoire d'études sur les monothéismes
2016
Toyohashi University of Technology
2016
Osaka Medical Center for Cancer and Cardiovascular Diseases
2006-2013
Soka University
2010-2011
Juntendo University
2011
Sumitomo Hospital
2010
Study Type – Prognostic (case series) Level of Evidence 4 What’s known on the subject? and What does study add? In general, tumour‐associated macrophage (TAM) is important for tumour progression including prostate cancer. this paper, we clarified usefulness TAM in biopsy specimens predicting prognosis after hormonal therapy. OBJECTIVE • To evaluate infiltration as a possible prognostic factor cancer (PCa) PATIENTS AND METHODS Immunostaining TAMs was performed using monoclonal antibody CD68...
Abstract Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. were isolated urine after massage 18 men (negative biopsy [n = 6], GS 6 PCa or 8–9 6]). EV proteins labeled with iTRAQ and analyzed by LC-MS/MS. identified 4710 quantified 3528 the EVs. Eleven increased patients compared those negative (ratio >1.5, p-value < 0.05)....
Purpose: High-fat diet (HFD) could induce prostate cancer progression. The aim of this study is to identify mechanisms HFD-induced progression, focusing on inflammation.Experimental Design: We administered HFD and celecoxib autochthonous immunocompetent Pb-Cre+;Pten(fl/fl) model mice for cancer. Tumor growth was evaluated by tumor weight Ki67 stain, local immune cells were assessed flow cytometry at 22 weeks age. Cytokines which correlated with identified, the changes after inhibition...
// Kyosuke Matsuzaki 1 , Kazutoshi Fujita Kentaro Jingushi 2 Atsunari Kawashima Takeshi Ujike Akira Nagahara Yuko Ueda Go Tanigawa 3 Iwao Yoshioka 4 Koji 5 Rikinari Hanayama 6 Motohide Uemura Yasushi Miyagawa Kazutake Tsujikawa Norio Nonomura Department of Urology, Osaka University Graduate School Medicine, Osaka, Japan Laboratory Molecular and Cellular Physiology, Pharmaceutical Science, General Medical Center, Police Hospital, Cancer Proteomics Group, Genome Japanese Foundation for...
Reliable biomarkers for renal cell carcinoma (RCC) have yet to be determined. Circulating tumor DNA (ctDNA) is an emerging resource detect and monitor molecular characteristics of various tumors. The present study aims clarify the clinical utility ctDNA RCC. Fifty‐three patients histologically diagnosed with clear RCC were enrolled. Targeted sequencing was carried out using plasma cell‐free (cfDNA) DNA. We applied droplet digital PCR (ddPCR) validate detected mutations. cfDNA fragment size...
Most upper tract urothelial carcinomas ( UTUC ) are muscle invasive at the time of diagnosis. Current standard methods for diagnosis invasive. Urine cytology is only non‐invasive test detecting , but its sensitivity low. A novel assay detection would improve patient outcome. This study aimed to investigate mutation cell‐free DNA (cf in urine supernatant develop a reliable diagnostic biomarker patients. We studied urinary cf from 153 individuals, including 56 patients with localized and...
BACKGROUND Fucosylation is an oligosaccharide modification associated with cancer and inflammation, which catalyzed by fucosyltransferases. Fucosylated haptoglobin (Fuc-Hpt) has been identified as a novel biomarker for pancreatic cancer. In this study, we evaluated serum Fuc-Hpt prostate cancer, investigated the expression of fucosyltransferases in cell lines. METHODS We measured preoperative levels 98 patients who underwent radical prostatectomy (RP) using established lectin-antibody ELISA....
Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource the diagnosis and prognosis of various cancers. This study aims identify novel blood RCC.Plasma cfDNA was extracted from RCC patients (n = 92) healthy controls 41). Levels were determined using quantitative real-time PCR ACTB as target gene, fragment size measured a microfluidics-based platform. Diagnostic potential assessed receiver operating characteristic...
Extracellular vesicles (EVs) including exosomes are present in blood, urine, and saliva contain proteins, microRNAs, messenger RNAs. We investigated microRNAs urinary EVs to discover new biomarkers of prostate cancer (PCa).We isolated from urine obtained following digital rectal examination (DRE) 14 men with elevated levels serum prostate-specific antigen (PSA) [negative biopsy (n=4) PCa Gleason scores 6 (n=3), 7 8-9 (n=4)]. MicroRNAs extracted were analyzed by microRNA microarray.MicroRNAs...
Chemoradiation therapy (CRT) is now widely recognized as bladder-preserving for muscle-invasive bladder cancer (MIBC). However, some patients who fail CRT may miss the chance to be cured by cystectomy. Therefore, it important select with MIBC are expected have a good response CRT. Several reports indicate that excision repair cross-complementing group 1 (ERCC1) gene associated resistance cisplatin and radiation therapy. In this study, we examined correlation between ERCC1 in vitro vivo...
Cryoablation is one of treatment modalities for kidney cancer and expected to induce strong local immune responses as well systemic T-cell-mediated reactions that may lead the regression distant metastatic lesions. Thus, characterization T cell repertoire environment in tumors before after should contribute better understanding cryoablation-induced anticancer responses. In this study, we collected tumor tissues from 22 patients, cryoablation at 3 mo cryoablation. addition, blood samples were...
Systemic inflammation and immune responses are reported to be associated with progressive prostate cancer. In this study, we explored which among the fractions of white blood cell (WBC) C-reactive protein (CRP) level were high Gleason score Prostate needle biopsy was performed in 966 men suspicion We assessed age, serum prostate-specific antigen (PSA), volume, WBC count, WBCs (neutrophils, lymphocytes, monocytes, basophils, eosinophils), CRP before for associations findings. Among all men,...
Abstract It is important to evaluate the clinical importance of both CD8 T cells and CD4 expression simultaneously because they have crucial networks in tumour targeting immune responses. In 97 RCC patients, RNA sequencing gene set enrichment analysis based on levels PD-1 TIM-3 implied that populations PD-1+TIM-3+ PD-1lowTIM-3 + were characterized as exhausted regulatory cells, respectively. These significantly upregulated patients with higher WHO/ISUP grade (grades 3, 4) ( P < 0.001)....
Although several studies have reported that microRNA (miR)-92b-3p is involved in various cellular processes related to carcinogenesis, its physiological role clear cell renal carcinoma (ccRCC) remains unclear. To clarify the of miR-92b-3p ccRCC, we compared expression levels ccRCC tissues and adjacent normal tissues. Significant upregulation was observed Overexpression using a miRNA mimic promoted proliferation, migration, invasion activities ACHN cells. Functional inhibition by hairpin...
Objectives: To review our series of testicular germ cell tumors with brain metastases and to establish an optimal treatment strategy for them. Methods: Twenty‐seven cases from three institutions were retrospectively reviewed. Results: Twenty‐six non‐seminomatous only one was a seminoma. Based on the International Germ Cell Consensus Classification, two classified as good prognosis, seven intermediate prognosis 18 poor prognosis. Chemotherapy carried out in all patients. Additionally,...
Despite an increasing prevalence of patients with docetaxel-refractory prostate cancer, little is known about the tumor biology docetaxel-resistant residual cells compared primary cells. In this study, tumorigenic potential was increased in cancer cell lines (DRD, 1G7 and PC3DR) parental (DU145 or PC3). Enhanced conferred by oncogenic c-Myc, which stabilized constitutively activated ERK1/2 DRD, 1G7, PC3DR Constitutively maintained CXCR4, upregulated docetaxel-treated DU145 cells, transiently...
Tumor-infiltrating macrophages, which are thought to be derived from blood monocytes, interact with tumor cells promote cancer progression. The aim of this study was assess the association peripheral monocyte count pathological findings and local tumor-infiltrating macrophages in prostatectomy specimens.Preoperative counts were retrospectively assessed for their associations (pathological T stage, Gleason Score, extraprostatic extension, seminal vesicle invasion, surgical margin) biochemical...
Fucosylation is an important oligosaccharide modification associated with cancer and inflammation. We investigated whether urinary fucosylated PSA (Fuc-PSA) levels could be used for the detection of high Gleason score prostate cancer. Urine samples were collected from men abnormal digital rectal examination findings or elevated serum levels, before biopsy. Lectin-antibody ELISA was to quantify Lewis-type core-type (PSA-AAL) (PSA-PhoSL) in urine samples. Both types Fuc-PSA significantly...