A5010097259- Gut microbiota and health
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Bladder and Urothelial Cancer Treatments
- Diet and metabolism studies
- Extracellular vesicles in disease
- Renal and related cancers
- Cancer, Lipids, and Metabolism
- Cancer Mechanisms and Therapy
- Cancer, Stress, Anesthesia, and Immune Response
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- Nutrition, Genetics, and Disease
- Inflammatory mediators and NSAID effects
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- interferon and immune responses
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Cancer-related gene regulation
- Cancer Immunotherapy and Biomarkers
- Circular RNAs in diseases
- Inflammatory Biomarkers in Disease Prognosis
- Immune cells in cancer
Osaka University
2016-2025
Suita Municipal Hospital
2018
Physiol (Belgium)
2016-2017
Cell Medica (Switzerland)
2017
Osaka University of Pharmaceutical Sciences
2015
Kyushu University
2008-2014
Hirosaki University
2006
Abstract The mammalian AlkB homolog (ALKBH) family of proteins possess a 2-oxoglutarate- and Fe(II)-dependent oxygenase domain. A similar domain in the Escherichia coli protein catalyzes oxidative demethylation 1-methyladenine (1-meA) 3-methylcytosine (3-meC) both DNA RNA. 3 (ALKBH3) was also shown to demethylate 1-meA 3-meC (induced single-stranded RNA by methylating agent) reverse methylation damage retain integrity DNA/RNA. We previously reported high expression ALKBH3 clinical tumor...
Excessive intake of animal fat and resultant obesity are major risk factors for prostate cancer. Because the composition gut microbiota is known to change with dietary body type, we used prostate-specific Pten knockout mice as a cancer model investigate whether there microbiota-mediated connection between Oral administration an antibiotic mixture (Abx) in cancer-bearing fed high-fat diet containing large proportion lard drastically altered including Rikenellaceae Clostridiales, inhibited...
Purpose: High-fat diet (HFD) could induce prostate cancer progression. The aim of this study is to identify mechanisms HFD-induced progression, focusing on inflammation.Experimental Design: We administered HFD and celecoxib autochthonous immunocompetent Pb-Cre+;Pten(fl/fl) model mice for cancer. Tumor growth was evaluated by tumor weight Ki67 stain, local immune cells were assessed flow cytometry at 22 weeks age. Cytokines which correlated with identified, the changes after inhibition...
Abstract Bladder cancer causes an estimated 150,000 deaths per year worldwide. Although 15% of the recurrent bladder becomes invasive type, currently used targeted therapy for malignant is still not efficient. We focused on miR-130 family (miR-130b, miR-301a and miR-301b) that was significantly upregulated in specimens than normal urothelial specimens. analyzed functional significance using a 5637 cell line revealed inhibitors suppressed migration invasion by downregulating focal adhesion...
// Kyosuke Matsuzaki 1 , Kazutoshi Fujita Kentaro Jingushi 2 Atsunari Kawashima Takeshi Ujike Akira Nagahara Yuko Ueda Go Tanigawa 3 Iwao Yoshioka 4 Koji 5 Rikinari Hanayama 6 Motohide Uemura Yasushi Miyagawa Kazutake Tsujikawa Norio Nonomura Department of Urology, Osaka University Graduate School Medicine, Osaka, Japan Laboratory Molecular and Cellular Physiology, Pharmaceutical Science, General Medical Center, Police Hospital, Cancer Proteomics Group, Genome Japanese Foundation for...
Reliable biomarkers for renal cell carcinoma (RCC) have yet to be determined. Circulating tumor DNA (ctDNA) is an emerging resource detect and monitor molecular characteristics of various tumors. The present study aims clarify the clinical utility ctDNA RCC. Fifty‐three patients histologically diagnosed with clear RCC were enrolled. Targeted sequencing was carried out using plasma cell‐free (cfDNA) DNA. We applied droplet digital PCR (ddPCR) validate detected mutations. cfDNA fragment size...
Cancer‐associated extracellular vesicles (EVs) are intimately involved in establishment of tumor microenvironment and occurrence metastasis. However, previous studies have mainly relied on experiments with cultured cell lines or mouse models, making it difficult to gain a full understanding EV functions human body. Hence, we extracted EVs directly from surgically resected viable clear renal carcinoma (ccRCC) tissues adjacent normal ( n = 20). Quantitative LC/MS analysis identified 3,871...
Abstract Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney, and clear RCC (ccRCC) represents its histological subtype. To identify a therapeutic target for ccRCC, miRNA expression signatures from ccRCC clinical specimens were analyzed. microarray real-time PCR analyses revealed that miR-629 was significantly upregulated in human compared with adjacent noncancerous renal tissue. Functional inhibition by hairpin inhibitor suppressed motility invasion. Mechanistically,...
Most upper tract urothelial carcinomas ( UTUC ) are muscle invasive at the time of diagnosis. Current standard methods for diagnosis invasive. Urine cytology is only non‐invasive test detecting , but its sensitivity low. A novel assay detection would improve patient outcome. This study aimed to investigate mutation cell‐free DNA (cf in urine supernatant develop a reliable diagnostic biomarker patients. We studied urinary cf from 153 individuals, including 56 patients with localized and...
Abstract Proteomic analysis of urinary extracellular vesicles (EVs) is a powerful approach to discover potential bladder cancer (BCa) biomarkers, however urine contains numerous EVs derived from the kidney and normal urothelial epithelium, which can obfuscate information related BCa cell‐derived EVs. In this study, we combined proteomic tissue‐exudative (Te‐EVs), were isolated culture medium freshly resected viable tissues. Urinary samples 11 individuals (7 patients 4 healthy individuals),...
Bacterial flora are present in various parts of the human body, including intestine, and thought to be involved etiology diseases such as multiple sclerosis, intestinal diseases, cancer, uterine diseases. In recent years, presence bacterial 16S rRNA genes has been revealed blood, which was previously a sterile environment, characteristic blood microbiomes have detected However, mechanism origin information unknown. this study, we performed metagenomic analysis DNA serum extracellular...
Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource the diagnosis and prognosis of various cancers. This study aims identify novel blood RCC.Plasma cfDNA was extracted from RCC patients (n = 92) healthy controls 41). Levels were determined using quantitative real-time PCR ACTB as target gene, fragment size measured a microfluidics-based platform. Diagnostic potential assessed receiver operating characteristic...
Extracellular vesicles (EVs) including exosomes are present in blood, urine, and saliva contain proteins, microRNAs, messenger RNAs. We investigated microRNAs urinary EVs to discover new biomarkers of prostate cancer (PCa).We isolated from urine obtained following digital rectal examination (DRE) 14 men with elevated levels serum prostate-specific antigen (PSA) [negative biopsy (n=4) PCa Gleason scores 6 (n=3), 7 8-9 (n=4)]. MicroRNAs extracted were analyzed by microRNA microarray.MicroRNAs...
Various factors have been implicated in the pathogenesis of ulcerative colitis (UC), with immune system failure being most important one. Calcitonin gene-related peptide (CGRP), a neuropeptide two isoforms, CGRPα and CGRPβ, has reported to regulate system. In this study, we investigated role CGRP isoforms UC pathogenesis. We induced UC-like symptoms CGRPβ knockout (KO) mice using dextran sulphate sodium. Compared wild-type KO mice, CGRPβ-deficient exhibited severe increased blood stool...
Non-small cell lung cancer (NSCLC) is the most frequent cause of cancer-related death worldwide. Although many molecular-targeted drugs for NSCLC have been developed in recent years, 5-year survival rate patients with remains low. Therefore, an improved understanding molecular mechanisms underlying biology essential developing novel therapeutic strategies treatment NSCLC. In this study, we examined role miR-130b Our results showed that high expression clinical specimens was significantly...
MicroRNAs (miRNAs) are noncoding RNAs that regulate gene expression and function in tumor development progression. We previously identified up-regulated miRNAs clear cell renal carcinoma (ccRCC) compared to matched-pair normal kidney by microarray. Here, we identify ccRCC also correlated with survival and/or recurrence. Twenty-four samples from patients who underwent nephrectomies between 2011 2012 were divided into two groups: one of eleven experienced recurrence (Group 1), thirteen no...
Clear cell renal carcinoma (ccRCC) is the most common histologically defined subtype of (RCC). To define molecular mechanism in progression ccRCC, we focused on LOX-like protein 2 (LOXL2), which critical for first step collagen and elastin cross-linking. Using exon array analysis quantitative validation, LOXL2 was shown to be significantly upregulated clinical specimens human ccRCC tumor tissues, compared with adjacent noncancerous this elevated expression correlated pathologic stages ccRCC....
Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney, and clear RCC (ccRCC) represents its histological subtype. Although several studies have reported high expression miR-122 in ccRCC, physiological role remains unclear. To clarify we compared levels non-cancerous tissue ccRCC. Significant upregulation was observed ccRCC specimens. Moreover, patients with showed poor progression-free survival to those low expression. Overexpression using an miRNA mimic promoted...
Although several studies have reported that microRNA (miR)-92b-3p is involved in various cellular processes related to carcinogenesis, its physiological role clear cell renal carcinoma (ccRCC) remains unclear. To clarify the of miR-92b-3p ccRCC, we compared expression levels ccRCC tissues and adjacent normal tissues. Significant upregulation was observed Overexpression using a miRNA mimic promoted proliferation, migration, invasion activities ACHN cells. Functional inhibition by hairpin...
Small extracellular vesicles (SEV) have attracted much attention both as mediators of intercellular communication and drug delivery systems. In addition, recent studies shown that SEV containing virus components particles are released from virus-infected cells. Oncolytic viruses, which efficiently kill tumor cells by cell-specific replication, been actively studied novel anticancer agents in clinical preclinical studies. However, it remains to be fully elucidated whether oncolytic involved...
Tumor-infiltrating macrophages, which are thought to be derived from blood monocytes, interact with tumor cells promote cancer progression. The aim of this study was assess the association peripheral monocyte count pathological findings and local tumor-infiltrating macrophages in prostatectomy specimens.Preoperative counts were retrospectively assessed for their associations (pathological T stage, Gleason Score, extraprostatic extension, seminal vesicle invasion, surgical margin) biochemical...
There are no blood biomarkers for the diagnosis of renal cell carcinoma ( RCC ) in routine clinical use. We focused on gene expression profile peripheral cells obtained from patients to discover novel diagnosis. Using microarray analysis and quantitative verification, CXCL 7 was shown be significantly upregulated patients. Importantly, aberrant confirmed even early stage pT 1a) patients, level a potential independent biomarker by receiver operating characteristic curve (sensitivity, 70.0%;...