A5018967580- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Cancer and Skin Lesions
- RNA regulation and disease
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- melanin and skin pigmentation
- Cancer-related gene regulation
- Colorectal Cancer Screening and Detection
- PI3K/AKT/mTOR signaling in cancer
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Wnt/β-catenin signaling in development and cancer
- Hippo pathway signaling and YAP/TAZ
- Microtubule and mitosis dynamics
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Glioma Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cellular Mechanics and Interactions
Kyushu University
2007-2024
Kagoshima University
2014-2024
Cancer Genetics (United States)
2012
The University of Tokyo
2000-2011
Osaka University
1998-2011
Kagoshima City Hospital
2009
Akita University
2007-2008
The functional impact of recently discovered long noncoding RNAs (ncRNAs) in human cancer remains to be clarified. One ncRNA which has attracted attention is the Hox transcript antisense intergenic RNA termed HOTAIR, a expressed from developmental HOXC locus located on chromosome 12q13.13. In cooperation with Polycomb complex PRC2, HOTAIR reported reprogram chromatin organization and promote breast metastasis. this study, we examined status function patients stage IV colorectal (CRC) who...
Background: The Wnt/Wingless signalling pathway plays an important role in both embryonic development and tumorigenesis. β‐Catenin Axin are positive negative effectors of the Wnt pathway, respectively. Results: We found that interacts with β‐catenin glycogen synthase kinase‐3β (GSK‐3β). Furthermore, regulation G‐protein (RGS) domain is associated colorectal tumour suppressor adenomatous polyposis coli (APC). Overexpression human cancer cell line SW480 induced a drastic reduction level...
PTEN is a tumor suppressor gene mutated in many human cancers. We generated bronchioalveolar epithelium–specific null mutation of Pten mice [SP-C-rtTA/(tetO)7-Cre/Ptenflox/flox (SOPtenflox/flox) mice] that was under the control doxycycline. Ninety percent SOPtenflox/flox received doxycycline utero [SOPtenflox/flox(E10–16) died hypoxia soon after birth. Surviving SOPtenflox/flox(E10–16) and postnatally [SOPtenflox/flox(P21–27) developed spontaneous lung adenocarcinomas. Urethane treatment...
Mps one binder 1a (MOB1A) and MOB1B are key components of the Hippo signaling pathway mutated or inactivated in many human cancers. Here we show that intact Mob1a Mob1b is essential for murine embryogenesis loss remaining WT Mob1 allele Mob1aΔ/Δ1btr/+ Mob1aΔ/+1btr/tr mice results tumor development. Because most these cancers resembled trichilemmal carcinomas, generated double-mutant bearing tamoxifen-inducible, keratinocyte-specific homozygous-null mutations (kDKO mice). kDKO showed...
Rationale: Injury to alveolar epithelial cells (AECs) and their repair process is integral the pathogenesis of acute lung injury (ALI) idiopathic pulmonary fibrosis (IPF). The mechanisms regulating integrity AECs intrinsic regulators remain unclear. Pten a tumor suppressor, its function in during organ unknown.Objectives: To determine role ALI fibrosis.Methods: Bronchioalveolar epithelium–specific Pten-deleted SP-C-rtTA/(tetO)7-Cre/PtenΔ/Δ (SOPtenΔ/Δ) mice were studied by structural,...
Although there is a relationship between DNA repair deficiency and temozolomide (TMZ) resistance in glioblastoma (GBM), it remains unclear which molecule associated with GBM recurrence. We isolated three TMZ-resistant human cell lines examined the expression of O6-methylguanine-DNA methyltransferase (MGMT) mismatch (MMR) components. used immunohistochemical analysis to compare MutL homolog 1 (MLH1), postmeiotic segregation increased 2 (PMS2) MGMT primary recurrent specimens obtained from...
Thymidine phosphorylase (TP), a rate-limiting enzyme in thymidine catabolism, plays pivotal role tumor progression; however, the mechanisms underlying this are not fully understood. Here, we found that TP-mediated catabolism could supply carbon source glycolytic pathway and thus contribute to cell survival under conditions of nutrient deprivation. In TP-expressing cells, was converted metabolites, including glucose 6-phosphate, lactate, 5-phospho-α-D-ribose 1-diphosphate, serine, via both...
Glioblastoma multiforme (GBM), the most common brain malignancy in adults, is generally aggressive and incurable, even with multiple treatment modalities agents. Filamins (FLNs) are a group of actin-binding proteins that regulate actin cytoskeleton cells. However, role FLNs malignancies—particularly GBM—is unclear. The relation between FLNC expression overall survival GBM was evaluated by Kaplan−Meier analysis using patients from Kagoshima University Hospital (n = 90) data Cancer Genome...
Non-small cell lung cancer (NSCLC) is the most frequent cause of cancer-related death worldwide. Although many molecular-targeted drugs for NSCLC have been developed in recent years, 5-year survival rate patients with remains low. Therefore, an improved understanding molecular mechanisms underlying biology essential developing novel therapeutic strategies treatment NSCLC. In this study, we examined role miR-130b Our results showed that high expression clinical specimens was significantly...
Abstract Phosphate and tensin homologue deleted on chromosome 10 (PTEN) is a tumor suppressor gene inactivated in numerous sporadic cancers, including melanomas. To analyze Pten functions melanocytes, we used the Cre-loxP system to delete specifically murine pigment-producing cells generated DctCrePtenflox/flox mice. Half of mice died shortly after birth with enlargements cerebral cortex hippocampus. Melanocytes were increased dermis perinatal When mutants subjected repeated depilations,...
Induced pluripotent stem cells (iPSCs) can be generated from patients with specific diseases by the transduction of reprogramming factors and useful as a cell source for transplantation therapy various impaired organs. However, low efficiency iPSC derived somatic (0.01-0.1%) is one major problems in field. The phosphoinositide 3-kinase (PI3K) pathway thought to important self-renewal, proliferation, maintenance embryonic (ESCs), but contribution this or its well-known negative regulator,...
Significance Much attention has been recently paid to the role of intestinal epithelial cells in homeostatic regulation immunity. Here we show that ablation stomach-cancer–associated protein tyrosine phosphatase 1 (SAP-1) markedly increased severity colitis interleukin (IL)-10–deficient mice, suggesting SAP-1 protects against a cooperative manner with IL-10. We also identify carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 20, an microvilli-specific membrane protein, as...
The tumor suppressor adenomatous polyposis coli (APC) is mutated in familial and sporadic colorectal tumors. APC forms a complex with β-catenin, Axin, glycogen synthase kinase-3β induces the degradation of β-catenin. In present study, we examined whether association Axin required for We found that fragment β-catenin was rendered inactive by disruption its Axin-binding sites. Also, overexpression an spanning regulator G-protein signaling domain inhibited APC-mediated degradation. An...
The TP53 pathway is frequently inactivated in human cancers. PICT1 (also known as GLTSCR2) a novel regulator of the MDM2-TP53 via its interaction with ribosomal protein RPL11 nucleolus. However, clinical significance gastric cancer remains unknown. To evaluate function, we used shRNA to inhibit expression cells that expressed wild-type TP53. and mutation status were quantified 110 cases primary explore impact levels on cancer. Deficiency significantly impaired cell proliferation colony...
To establish treatments to improve the prognosis of cancer patients, it is necessary find new targets control metastasis. We found that expression FilaminC (FLNC), a member actin binding and cross-linking filamin protein family correlated with lymphatic invasion metastasis in esophageal squamous cell carcinoma (ESCC) by increasing motility through activation Rho GTPase.Immunohistochemistry analysis showed FLNC ESCC associated invasion, metastasis, prognosis. knockdown lines decreased...
Abstract Considering the poor prognosis of most advanced cancers, prevention invasion and metastasis is essential for disease control. Ras homologous (Rho) guanine exchange factors (GEFs) their signaling cascade could be potential therapeutic targets in cancers. We conducted silico analyses The Cancer Genome Atlas expression data to identify candidate Rho-GEF genes showing aberrant gastric cancer found FERM, Rho/ArhGEF, pleckstrin domain protein 1 ( FARP1 ) related prognosis. Analyses 91...
Abstract The human AlkB homolog family (ALKBH) of proteins play a critical role in some types cancer. However, the expression and function lysine demethylase ALKBH4 cancer are poorly understood. Here, we examined non-small-cell lung (NSCLC) found that was highly expressed NSCLC, as compared to adjacent normal tissues. knockdown significantly induced downregulation NSCLC cell proliferation via cycle arrest at G 1 phase vivo tumour growth. downregulated E2F transcription factor (E2F1) its...
PICT1 is a key regulator of the MDM2-TP53 pathway. High mRNA expression levels are associated with poor prognosis in several cancers wild-type TP53. In this study, we identified protein profile non-small cell lung cancer (NSCLC) TP53 nucleolus and cytoplasm, revealed relationship between patient clinicopathological factors. tumor cells 96 NSCLC patients was evaluated by immunohistochemistry. Forty-three (44.8%) samples were positive for nucleolar PICT1, while 40/96 (41.7%) cytoplasmic PICT1....
Glioblastoma multiforme (GBM), the most common primary malignant brain tumor in adults, is characterized by rapid proliferation, aggressive migration, and invasion into normal tissue. Formin proteins have been implicated these processes. However, role of formin-like 1 (FMNL1) cancer remains unclear. We studied FMNL1 expression glioblastoma samples using immunohistochemistry. sought to analyze correlation between expression, clinicopathologic variables, patient survival. Migration assays were...
Thymidine phosphorylase (TP) is a rate-limiting enzyme in the thymidine catabolic pathway. TP identical to platelet-derived endothelial cell growth factor and contributes tumour angiogenesis. induces generation of reactive oxygen species (ROS) enhances expression oxidative stress-responsive genes, such as interleukin (IL)-8. However, mechanism underlying ROS induction by remains unclear. In present study, we demonstrated that promotes NADPH oxidase-derived signalling cancer cells. oxidase...
The activation of yes-associated protein 1 (YAP1) and transcriptional co-activator with PDZ-binding motif (TAZ) has been implicated in both regeneration tumorigenesis, thus representing a double-edged sword tissue homeostasis. However, how the activity YAP1/TAZ is regulated or what leads to its dysregulation these processes remains unknown. To explore upstream stimuli modulating cellular YAP1/TAZ, we developed highly sensitive YAP1/TAZ/TEAD-responsive DNA element (YRE) incorporated it into...