A5025137523- Lung Cancer Treatments and Mutations
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lung Cancer Research Studies
- Immunotherapy and Immune Responses
- Occupational and environmental lung diseases
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Medical Imaging and Pathology Studies
- Cytokine Signaling Pathways and Interactions
- Lung Cancer Diagnosis and Treatment
- Pleural and Pulmonary Diseases
- Immune cells in cancer
- PI3K/AKT/mTOR signaling in cancer
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Cancer Mechanisms and Therapy
- Cancer-related gene regulation
- Sarcoidosis and Beryllium Toxicity Research
- Cancer therapeutics and mechanisms
- Pancreatic and Hepatic Oncology Research
- Lymphatic System and Diseases
- Systemic Sclerosis and Related Diseases
- interferon and immune responses
- NF-κB Signaling Pathways
Tokushima University
2016-2025
Tokushima University Hospital
2005-2024
Hôpital Cochin
2023
Assistance Publique – Hôpitaux de Paris
2023
Isabela State University
2023
Université Paris Cité
2023
Juntendo University
2015-2021
Center for Rheumatology
2008-2021
Kumamoto University
2021
Kyoto University
2021
Abstract Lung cancer with epidermal growth factor receptor (EGFR)–activating mutations responds favorably to the EGFR tyrosine kinase inhibitors gefitinib and erlotinib. However, 25% 30% of patients EGFR-activating show intrinsic resistance, responders invariably acquire resistance gefitinib. Here, we showed that hepatocyte (HGF), a ligand MET oncoprotein, induces lung adenocarcinoma cells by restoring phosphatidylinositol 3-kinase/Akt signaling pathway via phosphorylation MET, but not or...
Lung cancers with epidermal growth factor receptor (EGFR)-activating mutations show good clinical response to gefitinib and erlotinib, selective tyrosine kinase inhibitors (TKI) EGFR, but these tumors invariably develop drug resistance. Host stromal cells have been found a considerable effect on the behavior of cancer cells. Little is known, however, about role host sensitivity TKIs. We therefore assessed crosstalk between lung harboring EGFR susceptibility EGFR-TKIs.We evaluated...
We examined whether macrophage infiltration is associated with angiogenesis in cutaneous melanoma. The numbers of macrophages and microvessels increased significantly increasing depth tumor angiogenesis. Macrophage thus appeared to provide a useful diagnostic marker for the progression further human melanoma cells produce angiogenic factors response macrophage-derived cytokines, necrosis factor alpha (TNFα) interleukin-1 (IL-1α). Treatment TNFα IL-1α vitro enhanced production interleukin-8...
We have cloned and determined the nucleotide sequence of a mouse alpha-globin-like gene that entirely lacks two intervening sequences interrupt all globin genes thus far examined. The fact this gene, alpha-3, is closely homologous to normal adult alpha suggests it arose after alpha/beta divergence therefore must lost its sequences. further these been cleanly--that is, in according with G-T/A-G splicing rule RNA--suggests, among other possibilities, their loss may brought about by conversion...
Lysophosphatidic acids (LPA) with a C18 fatty acyl group accelerated thymidine incorporation into cultured rat aortic vascular smooth muscle cells and stimulated their cell division. LPA acted synergistically epidermal growth factor fibroblast but additively platelet-derived factor. The stimulatory actions of were suggested to be rather specific from the following findings: 1) stimulation DNA synthesis increased an increase in moiety; 2) lysophosphatidylcholine, neutral lysophospholipid, had...
FIG. 3. DNA-sequencing gels showing the determination of repeating CA sequence.Panels I and II represent sense anti-sense strand, respectively.
Background. Dendritic cells (DC) play critical roles in the initiation and modulation of immune responses may determine balance between tolerance immunity. Viral intreleukin-10 (vIL-10), encoded by Epstein-Barr virus, is highly homologous to "immunosuppressive" cytokine, mammalian IL-10. It impairs antigen-presenting cell function but lacks certain immunostimulatory properties We accomplished following: (1) evaluated effects vIL-10 protein on DC phenotype function, (2) transduced mouse bone...
Stimulation of glucose transport is the main physiological effect insulin in target tissues. This linked to translocation GLUT4 transporter from an intracellular pool cell surface. To elucidate molecular mechanisms involved this effect, we developed a simple direct sensitive method detect immunologically on cDNA containing inserted by c-myc epitope first ectodomain (GLUT4myc) was constructed without disrupting functions and expressed 3T3-L1 Chinese hamster ovary fibroblast cells. In response...
The fate of preexisting mRNA sequences was examined after infection by herpes simplex virus. Murine erythroid cells transformed Friend leukemia virus were used as the host. Such cells, when exposed to 2% dimethyl sulfoxide, produce large amounts globin and mRNA. protein its are easily recognized at 4 days electrophoresis in high percentage acrylamide gels hybridization cDNA, respectively. Herpes replicates these cells. By 2 hr rate synthesis decreases 30% level mock-infected only 49+/-8%...
We describe experiments which demonstrate that shortly after infection of Friend erythroleukemia cells with herpes simplex virus (HSV), polyribosomes dissociate and cellular mRNA degrades. Analysis infected cell extracts on sucrose density gradients demonstrates the majority have dissociated to monoribosomes at 2 h postinfection. Physical measurements infected-cell RNAs support this conclusion polyadenylated decrease in size. The degradation is apparently a stochastic process as judged by...
Abstract Purpose: Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI), such as gefitinib and erlotinib, show favorable response to EGFR mutant lung cancer. However, the responders acquire resistance almost without exception. We recently reported that hepatocyte (HGF) induces EGFR-TKI by activating MET restores downstream mitogen activated protein (MAPK)/extracellular signal regulated (ERK)1/2 phosphoinositide 3-kinase (PI3K)/Akt signaling. The purpose of this study was...
Abstract Purpose: The secondary T790M mutation in epidermal growth factor receptor (EGFR) is the most frequent cause of acquired resistance to reversible EGFR tyrosine kinase inhibitors (EGFR-TKI), gefitinib and erlotinib, lung cancer. Irreversible EGFR-TKIs are expected overcome EGFR-TKI cancer harboring EGFR. However, it clear that may also develop this class inhibitors. We showed previously hepatocyte (HGF) induced EGFR-activating mutations. Here, we investigated whether HGF irreversible...
Podoplanin (Aggrus), which is a type I transmembrane sialomucin-like glycoprotein, highly expressed in malignant pleural mesothelioma (MPM). We previously reported the generation of rat anti-human podoplanin Ab, NZ-1, inhibited podoplanin-induced platelet aggregation and hematogenous metastasis. In this study, we examined antitumor effector functions NZ-1 NZ-8, novel rat-human chimeric Ab generated from including Ab-dependent cellular cytotoxicity (ADCC) complement-dependent against MPM...
Abstract Purpose: Hepatocyte growth factor (HGF) induces resistance to reversible and irreversible epidermal receptor–tyrosine kinase inhibitors (EGFR-TKI) in EGFR mutant lung cancer cells by activating Met the downstream phosphoinositide 3-kinase (PI3K)/Akt pathway. Moreover, continuous exposure HGF accelerates emergence of EGFR-TKI–resistant clones. We assayed whether a new inhibitor, E7050, which is currently being evaluated clinical trials, could overcome these three mechanisms...
Podoplanin is a platelet aggregation‐inducing factor associated with tumor metastasis, malignant progression, and cancer stem cells. We produced rat–human chimeric anti‐podoplanin m A b, NZ ‐8, from rat b ( ‐1). Although both ‐1 ‐8 possess high binding affinities neutralizing activities of aggregation, the antibody‐dependent cellular cytotoxicity complement‐dependent were much higher than ‐1. Furthermore, inhibited growth podoplanin‐expressing tumors in vivo . Both also suppressed...
Abstract EGFR tyrosine kinase inhibitors cause dramatic responses in EGFR-mutant lung cancer, but resistance universally develops. The involvement of β-catenin TKI has been previously reported, however, the precise mechanism by which activation contributes to is not clear. Here, we show that inhibition results signaling a Notch3-dependent manner, facilitates survival subset cells call “adaptive persisters”. We reported EGFR-TKI treatment rapidly activates Notch3, and here describe physical...
Abstract The p16 INK4a tumour suppressor has an established role in the implementation of cellular senescence stem/progenitor cells, which is thought to contribute organismal ageing. However, since knockout mice die prematurely from cancer, whether reduces longevity remains unclear. Here we show that, mutant homozygous for a hypomorphic allele α-klotho ageing-suppressor gene ( kl kl/kl ), accelerated ageing phenotypes are rescued by ablation. Surprisingly, this due restoration expression and...
Bevacizumab exerts anti-angiogenic effects in cancer patients by inhibiting vascular endothelial growth factor (VEGF). However, its use is still limited due to the development of resistance treatment. Such can be regulated various factors, although underlying mechanisms remain incompletely understood. Here we show that bone marrow-derived fibrocyte-like cells, defined as alpha-1 type I collagen-positive and CXCR4-positive contribute acquired bevacizumab. In mouse models malignant pleural...
Abstract Immune checkpoint inhibitors (ICIs) elicit antitumour effects by activating the host immunity and cause immune-related adverse events (irAEs). ICI-related interstitial lung disease (ICI-ILD) is a fatal irAE that difficult to treat; moreover, its incidence relatively higher in patients with cancer. Therefore, early ICI-ILD detection intervention are important for patient safety. However, risk assessment method has not been established prediction of occurrence difficult. The aim our...