A5060693402- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Cancer Research and Treatments
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- PARP inhibition in cancer therapy
- Epigenetics and DNA Methylation
- FOXO transcription factor regulation
- Cancer-related gene regulation
- Lung Cancer Treatments and Mutations
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Genomics and Chromatin Dynamics
- BRCA gene mutations in cancer
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Cancer therapeutics and mechanisms
- Avian ecology and behavior
- Ubiquitin and proteasome pathways
- Colorectal Cancer Treatments and Studies
Florida International University
2017-2022
The Ohio State University
2011-2021
Shaanxi University of Science and Technology
2021
University of Manchester
2019
The Ohio State University Wexner Medical Center
2007-2018
National Cancer Institute
2016
Georgetown University Medical Center
2016
Georgetown University
2016
Cancer Genetics (United States)
2016
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2003-2016
DNA methylation in the promoter of certain genes is associated with transcriptional silencing. Methylation affects gene expression directly by interfering transcription factor binding and/or indirectly recruiting histone deacetylases through methyl-DNA-binding proteins. In this study, we demonstrate that human lung cancer cell line H719 lacks p53-dependent and -independent p21(Cip1) expression. p53 response to treatment gamma irradiation or etoposide lost due a mutation at codon 242 (C-->W)....
In addition to its demethylating function, 5-aza-2′-deoxycytidine (5-aza-CdR) also plays an important role in inducing cell cycle arrest, differentiation, and death. However, the mechanism by which 5-aza-CdR induces antineoplastic activity is not clear. this study, we found that at limited concentrations (0.01–5 μm) inhibition of proliferation as well increased p53/p21Waf1/Cip1 expression A549 cells (wild-type p53) but H1299 (p53-null) H719 (p53 mutant). The p53-dependent p21Waf1/Cip1...
Cancer stem cells (CSCs) have been identified in hematopoietic and solid tumors. However, their precursors—namely, precancerous (pCSCs) —have not characterized. Here we experimentally define the pCSCs that potential for both benign malignant differentiation, depending on environmental cues. While clonal can develop into various types of tissue immunocompetent mice without developing cancer, they often develop, however, leukemic or cancers composed cancer immunodeficient mice. The progress to...
Abstract EGFR tyrosine kinase inhibitors cause dramatic responses in EGFR-mutant lung cancer, but resistance universally develops. The involvement of β-catenin TKI has been previously reported, however, the precise mechanism by which activation contributes to is not clear. Here, we show that inhibition results signaling a Notch3-dependent manner, facilitates survival subset cells call “adaptive persisters”. We reported EGFR-TKI treatment rapidly activates Notch3, and here describe physical...
Maintenance of p53 function is important for normal cell growth and development, loss contributes directly to malignant tumor development. The recently discovered Pirh2 protein an ubiquitin–protein ligase that negatively regulates through activity by targeting it degradation. To determine how may mediate lung tumorigenesis, we evaluated expression in human mouse samples paired tissues using immunoblot analysis immunohistochemistry. was higher 27 (84%) 32 neoplasms than matched tissue 14 15...
DNA damage response (DDR) is an intrinsic barrier of cell to tumorigenesis initiated by genotoxic agents. However, the mechanisms underlying DDR are not completely understood despite extensive investigation. Recently, we have reported that ectopic expression germline stem gene PIWIL2 associated with tumor development, although largely unknown. Here show required for repair DNA-damage induced various types Upon ultraviolet (UV) irradiation, silenced in normal human fibroblasts was transiently...
Protein arginine methyltransferase-5 (PRMT5) is a chromatin-modifying enzyme capable of methylating histone and non-histone proteins, involved in wide range cellular processes that from transcriptional regulation to organelle biosynthesis. As such, its overexpression has been linked tumor suppressor gene silencing, enhanced cell growth survival. Quantitative real-time polymerase chain reaction, Western immunoblot immunohistochemistry were used characterize PRMT5 expression lung cancer lines...
Rationale: The mitogen-activated protein kinase pathway (MAPK) is one of the major cancer-driving pathways found in non-small cell lung cancer (NSCLC) patients. ERK inhibitors (ERKi) have been shown to be effective NSCLC patients with MAPK mutations. However, like other inhibitors, ERKi rarely confers complete and durable responses. mechanism tumor relapse after treatment yet defined. Methods: To best study inhibitor patients, we treated various lines patient-derived xenograft (PDX)...
The Fanconi anemia (FA) pathway is a major mechanism of homologous recombination DNA repair. functional readout the activation through mono-ubiquitination FANCD2 leading to nuclear foci We have recently developed an FA triple-staining immunofluorescence based method (FATSI) evaluate formation in formalin fixed paraffin-embedded (FFPE) tumor samples. DNA-repair deficiencies been considered interest lung cancer prevention, given persistence damage produced by cigarette smoke this setting, as...
// Tiantian Cui 1 , Amit Kumar Srivastava Chunhua Han Linlin Yang 2 Ran Zhao Ning Zou Meihua Qu Wenrui Duan 3 Xiaoli Zhang 4 and Qi-En Wang Department of Radiology, The Ohio State University Wexner Medical Center, Columbus, OH, USA Radiation Oncology, Internal Medicine, Center for Biostatistics, Correspondence to: Wang, email: Keywords : XPC, E-Cadherin, non-small-cell lung cancer, Snail, ERK pathway Received December 28, 2014 Accepted February 13, 2015 Published March 12, Abstract Xeroderma...
The ER plays an important role in the proliferation and differentiation of lactotrope tumor cells. GH4 cells were infected with adenoviral vectors (AdL540Q Ad1–536) to investigate ability dominant negative mutants affect regulation gene expression cell growth by endogenous ER. suppressed estradiol stimulation estrogen-responsive reporter PRL promoter these AdL540Q or Ad1–536 infection also inhibited induced apoptosis, increasing proapoptotic Bax protein decreasing antiapoptotic Bcl-2....
Significance The members of the rat sarcoma (RAS) gene family Kirsten viral oncogene homolog, Harvey and neuroblastoma RAS homolog (NRAS) belong to most extensively studied oncogenes are central players in carcinogenesis. Since their discovery approximately 30 y ago, efforts target aberrant activation have not led major breakthroughs. We herein report four so far undescribed variants NRAS that differ expression patterns and, strikingly, downstream effects. Our results suggest should be...
Abstract We estimated the rate of extra‐pair fertilizations (EPFs) in waved albatrosses ( Phoebastria irrorata ) on Isla Española, Galápagos, Ecuador, using multilocus minisatellite DNA fingerprinting. Waved are socially monogamous, long‐lived seabirds whose main population is Española. Aggressive copulation (EPC) attempts have been observed breeding colony during days preceding egg‐laying. Our genetic analyses 16 families (single chicks and their attending parents) revealed evidence EPFs...
The presence of transthyretin in mammals and birds, but not amphibia, suggested that expression first appeared stem reptiles. Therefore, synthesis was studied a lizard. Transthyretin choroid plexus pieces from Tiliqua rugosa demonstrated by incorporation radiactive amino acids. Oligonucleotides corresponding to conserved regions were used as primers polymerase chain reaction with lizard cDNA. Amplified DNA screen cDNA library. A full-length clone isolated sequenced. three-dimensional model...
Restoration of p53 function in tumor cells would be an attractive strategy for lung cancer therapy because mutations are found more than 50% cancers. The small molecule PRIMA-1 has been shown to restore the suppression and induce apoptosis human cells. mechanism induced by remains unclear. We investigated effects with Western immunoblot analysis, TaqMan microRNA real-time PCR, cell viability analysis flow cytometry using lines containing mutant (H211 H1155), wild-type (A549) or null (H1299)...
BRCA germline mutations are being targeted for development of PARP inhibitors. genes collaborate with several others in the Fanconi Anemia (FA) pathway. We screened cancer patients' tumors FA functional defects then aimed to establish safety/feasibility administering inhibitors as monotherapy and combined a DNA-breaking agent. Patients underwent screening presence (or lack) tumor FancD2 nuclear foci formation on their archival material, utilizing newly developed method (Fanconi triple-stain...
A female-specific DNA fragment (CSL-W; crane sex-linked on W chromosome) was cloned from female whooping cranes (Grus americana). From the nucleotide sequence of CSL-W, a set polymerase chain reaction (PCR) primers identified which amplify 227–230 bp all existing species and some other noncrane species. duplicated versions segment, is found to have larger size (231–235 bp) than CSL-W in both sexes, also identified, designated CSL-NW (crane non-W chromosome). The similarity between sequences...