A5064363754- Glycosylation and Glycoproteins Research
- Sulfur Compounds in Biology
- Molecular Sensors and Ion Detection
- Cancer, Lipids, and Metabolism
- Influenza Virus Research Studies
- Galectins and Cancer Biology
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Lipid metabolism and biosynthesis
- Hepatitis C virus research
- Liver physiology and pathology
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Endoplasmic Reticulum Stress and Disease
- Respiratory viral infections research
- Cholesterol and Lipid Metabolism
- Redox biology and oxidative stress
- Luminescence and Fluorescent Materials
- Peroxisome Proliferator-Activated Receptors
- Salivary Gland Disorders and Functions
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Folate and B Vitamins Research
- Cancer Research and Treatments
- Diabetes and associated disorders
Northwest University
2012-2024
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2020-2024
The Ohio State University
2019-2024
First Affiliated Hospital of Guangdong Pharmaceutical University
2024
Affiliated Hospital of Guizhou Medical University
2024
Guiyang Medical University
2024
University College London
2024
Wuhan University
2023
Zhongnan Hospital of Wuhan University
2023
Chaoyang University of Technology
2021
Many studies have shown that glutathione (GSH) and cysteine (Cys)/homocysteine (Hcy) levels are interrelated in biological systems. To unravel the complicated biomedical mechanisms by which GSH Cys/Hcy involved various disease states, probes display distinct signals response to highly desirable. In this work, we report a rhodol thioester (1) responds with fluorescence emissions neutral media. Probe 1 reacts form corresponding deconjugated spirolactam via tandem native chemical ligation (NCL)...
Spirocyclization of xanthene dyes has become a powerful technique for developing fluorescent probes. Herein, we extend this unique fluorescence switching mechanism to near-infrared (NIR) dye, 2-(7-diethylamino-2-oxo-2H-1-benzopyran-3-yl)-4-(2-carboxyphenyl)-7-diethylamino-1-benzopyrylium (CB), and construct ratiometric probe 1 cysteine (Cys)/homocysteine (Hcy). The sensing toward Cys/Hcy is realized by utilizing tandem native chemical ligation/spirocyclization reaction interrupt the large...
Selenocysteine (Sec) carries out the majority of functions various Se-containing species in vivo. Thus, it is great importance to develop sensitive and selective assays detect Sec. Herein, a carbon-dot-based fluorescent turn-on probe for highly detection selenol living cells presented. The photoluminescent carbon dots that emit yellow-green fluorescence (Y-G-CDs; λmax = 520 nm water) were prepared by using m-aminophenol as precursor through facile solvothermal method. surface Y-G-CDs was...
Recently, lipid metabolism reprogramming has been further evidenced in malignancies via the observation of large amounts droplets (LDs) human tumors, including glioblastoma (GBM), most lethal primary brain tumor. However, role played by LDs tumor cells remains unknown. Here, we show that triglycerides (TG), major components LDs, serve as a critical energy reservoir to support GBM cell survival. TG/LDs rapidly diminished upon glucose reduction, whereas inhibiting fatty acid oxidation or...
Recent studies have elucidated that expression of certain glycoproteins in human saliva is increased or decreased according to age; meanwhile, may inhibit viral infection and prevent transmission. However, little known about the age- sex-associated differences glycopatterns salivary their significant roles against influenza A virus (IVA). Here, we investigate with 180 healthy samples divided into six age/sex groups using lectin microarrays fabricate validate terminal carbohydrate moieties...
Cholesterol is a structural component of cell membranes. How rapidly growing tumor cells maintain membrane cholesterol homeostasis poorly understood. Here, we found that glioblastoma (GBM), the most lethal brain tumor, maintains normal levels but with an abundant presence cholesteryl esters (CEs) in its lipid droplets (LDs). Mechanistically, SREBP-1 (sterol regulatory element-binding protein 1), master transcription factor activated upon depletion, upregulates critical autophagic genes,...
SCAP plays a central role in controlling lipid homeostasis by activating SREBP-1, master transcription factor fatty acid (FA) synthesis. However, how expression is regulated human cancer cells remains unknown. Here, we revealed that STAT3 binds to the promoter of activate its across multiple cell types. Moreover, identified also concurrently interacts with SREBF1 gene (encoding SREBP-1), amplifying expression. This dual action collaboratively heightens FA Pharmacological inhibition...
Abstract Checkpoint kinase 1 (CHK1) is critical for cell survival under replication stress (RS). CHK1 inhibitors (CHK1i’s) in combination with chemotherapy have shown promising results preclinical studies but displayed minimal efficacy substantial toxicity clinical trials. To explore combinatorial strategies that can overcome these limitations, we perform an unbiased high-throughput screen a non-small lung cancer (NSCLC) line and identify thioredoxin1 (Trx1), major component of the mammalian...
Protein glycosylation plays an important role in the pathogenesis and progression of various liver diseases. However, little is known about precise alterations protein or potential correlation between glycan-related genes expression glycan profiles fibrosis. The aim study was to investigate associations evaluate fibrosis a mouse model. Analyses were performed using oligonucleotide microarrays lectin microarrays, respectively. Real-time PCR Western blot used confirm any altered levels levels....
Two glycoproteins, hemagglutinin (HA) and neuraminidase (NA), on the surface of influenza viruses play crucial roles in transfaunation, membrane fusion release progeny virions. To explore distribution N-glycosylation sites (glycosites) these two we collected aligned amino acid sequences all HA NA subtypes. glycosites were located at HA0 cleavage peptides strikingly conserved subtypes, while remaining unique to their four found stalk domain NA, but are affected by deletion specific sequences....
Saliva proteins may protect older people from influenza, however, it is often noted that hospitalizations and deaths after an influenza infection mainly occur in the elderly population living with chronic diseases, such as diabetes cancer. Our objective was to investigate expression level of terminal α2-3- α2-6-linked sialic acids human saliva type 2 mellitus (T2DM), liver disease gastric cancer (GC) patients assess binding activity these linked against A viruses (IAV). We observed...
Vicinal dithiol-containing proteins (VDPs) play a significant role in maintaining the cellular redox homeostasis and are implicated many diseases. To provide new chemical tools for VDPs imaging, we report here ratiometric fluorescent probe CAsH2 using 7-diethylaminiocoumarin as reporter cyclic 1,3,2-dithiarsenolane specific ligand. shows peculiar dual fluorescence emission from excited intramolecular charge transfer (ICT) twisted (TICT) states aqueous media. However, upon selective binding...
An environment-sensitive red fluorescent light-up probe for vicinal dithiol-containing proteins (VDPs) in living cells has been successfully developed.
Glycoproteins comprise a large portion of the salivary proteome and have great potential for biomarker discovery disease diagnosis. However, rate production concentration whole saliva change with age, gender physiological states human body. Therefore, thorough understanding glycoproteome healthy individuals different ages genders is prerequisite to clinical utility.Formerly N-linked glycopeptides were isolated from pooled six age groups by hydrazide chemistry hydrophilic affinity methods...
A flavylium-based turn-on fluorescent probe for imaging of hydrogen polysulfides in living cells has been developed.