A5065322854- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Coronary Interventions and Diagnostics
- Cardiovascular and exercise physiology
- Cardiovascular Function and Risk Factors
- Cardiovascular Disease and Adiposity
- Cardiac Imaging and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Cardiovascular Health and Disease Prevention
- Diet and metabolism studies
- Muscle metabolism and nutrition
- Genetic factors in colorectal cancer
- Metabolism and Genetic Disorders
- Immune Response and Inflammation
- Atherosclerosis and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Iron Metabolism and Disorders
- Nerve injury and regeneration
- Blood Coagulation and Thrombosis Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Signaling Pathways in Disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pleural and Pulmonary Diseases
- ATP Synthase and ATPases Research
- Metabolomics and Mass Spectrometry Studies
Hokko Memorial Hospital
2023
Hokkaido University
2014-2021
Hakodate National Hospital
2014-2016
Brigham and Women's Hospital
2012-2015
Shonan Kamakura General Hospital
2012-2015
Harvard University
2012-2013
Tokai University
1989-2001
Abstract Background Exercise intolerance is a common clinical feature and linked to poor prognosis in patients with heart failure (HF). Skeletal muscle dysfunction, including impaired energy metabolism the skeletal muscle, suspected play central role this intolerance, but underlying mechanisms remain elusive. Lysine acetylation, recently identified post‐translational modification, has emerged as major contributor derangement of mitochondrial metabolism. We thus investigated whether protein...
Transverse aortic constriction (TAC) operation is used as an experimental model of left ventricular (LV) hypertrophy and LV failure in mice. The severity remodeling or may depend on the degree TAC, but variable among operated animals. Therefore, we tried to identify optimal diameter TAC create this with ease high reproducibility.To produce C57BL/6J mice (7-9 weeks, body weight 19-26 g, n = 109), a 7-0 nylon suture ligature was tightly tied around transverse aorta against needles 3 different...
Exercise intolerance in patients with heart failure (HF) is partly attributed to skeletal muscle abnormalities. We have shown that reactive oxygen species (ROS) play a crucial role abnormalities, but the pathogenic mechanism remains unclear. Xanthine oxidase (XO) reported be an important mediator of ROS overproduction ischaemic tissue. Here, we tested hypothesis abnormalities HF are initially caused by XO-derived and prevented inhibition their production.
Abstract Heart failure (HF) occurs frequently among older individuals, and dysfunction of cardiac mitochondria is often observed. We here show the cardiac-specific downregulation a certain mitochondrial component during chronological aging mice, which detrimental to heart. MitoNEET outer membrane protein, encoded by CDGSH iron sulfur domain 1 (CISD1). Expression mitoNEET was specifically downregulated in heart kidney chronologically aged mice. Mice with constitutive deletion CISD1 on...
Abstract Bredinin inhibition of cell growth was investigated in the mouse lymphoma line L5178Y. caused accumulation IMP and reduction XMP. It converted to 5′‐phosphate within cells. but not bredinin competitively inhibited both dehydrogenase GMP synthetase. Thus is probably due 5′‐phosphate, which inhibits consecutive enzyme reactions These inhibitions result
A method of separating neutrophils from the peripheral blood rats with 95% purity is described. To determine role antigenic stimulation in neutrophil function, front germ‐free (GF) were compared those conventional (CV) rats. Neutrophil counts lower GF but total number monocytic cells was same. measure phagocytic killing, superoxide anion production determined and found to be (2.1 ± 0.5 nmol/min. 10 6 cells) than CV (9.5 2.9 nmol/min/10 cells). Myeloperoxidase activity twofold higher...
Background In‐stent hyperplasia ( ISH ) may develop in regions of low endothelial shear stress ESS ), but the relationship between magnitude , extent ISH, and subsequent clinical events has not been investigated. Methods Results We assessed association poststent with neointimal outcomes patients treated percutaneous coronary interventions PCI ). Three‐dimensional reconstruction was performed 374 post‐ at baseline 6 to 10 months follow‐up as part PREDICTION Study. Each vessel divided into...
A 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having congenital absence the portal vein. The blood ammonia level (approximately 120 micrograms dl-1) in superior mesenteric vein markedly low compared to normal value 300-350 dl-1 decreased concentration and urease activity patient's faeces were demonstrated. dominant intestinal flora before operation, Bifidobacterium sp., breve, lonqum, Lactobacillus plantarum, after...
Skeletal muscle atrophy is induced by an imbalance between protein synthesis and degradation. Our previous studies reported that angiotensin II (AII) directly in mice. This study investigated the role of NAD(P)H oxidase 2 (Nox2) activation AII induction skeletal atrophy. For 4 weeks, either saline (vehicle: V) or (1000 ng kg −1 min ) was infused into male wild-type (WT) Nox2 knockout (KO) mice via osmotic minipumps. Experiments were performed following groups: WT + V, KO AII, AII. Body...
A 54-year-old man with polycystic liver disease received a domino transplantation (DLT) from patient of hereditary ATTR amyloidosis the transthyretin Ser50Arg mutation. Ten years after transplantation, he felt slight numbness in his toes, and cardiac was simultaneously suspected upon heart function evaluation. Biopsy specimens myocardium revealed Oral tafamidis therapy has inhibited progression neurological cardiovascular symptoms this far. We herein report first case amyloid polyneuropathy...
Peripheral blood neutrophils from germ‐free (GF), specific‐pathogen‐free (SPF) and conventional (CV) rats were compared. Besides neutropenia impaired superoxide anion generation as previously reported, it was found that GF had lower phagocytic function (70%) generated less nitric oxide than the other rats. SPF injected with recombinant human granulocyte–colony‐stimulating factor (rhG‐CSF), or transferred to natural environment. It total number, phagocytosis, intracellular killing, ratio of...
Congenitally abnormal plasminogen is characterized by markedly decreased fibrinolytic activity and has been reported mainly in association with venous occlusive disease. We found three young adult patients (34, 45, 27 years old at onset) ischemic cerebrovascular disease, all of whom had congenital abnormalities but no other known risk factors. Hemostatic tests revealed plasma activities almost one half the normal level despite antigen levels. They were to be heterozygotes (normal...
Abstract Iron is an essential trace element for regulation of redox and mitochondrial function, then iron content tightly regulated in mammals. We focused on a novel protein localized at the outer membrane. Immunoelectron microscopy revealed transferrin receptor (TfR) displayed intimate relationship with mitochondria, mass spectrometry analysis also mitoNEET interacted TfR vitro . Moreover, was endogenously coprecipitated heart, which indicates that interacts vivo generated mice...