A5061357676- Autoimmune Bullous Skin Diseases
- Urticaria and Related Conditions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Celiac Disease Research and Management
- Nail Diseases and Treatments
- Psoriasis: Treatment and Pathogenesis
- Phytochemical compounds biological activities
- Dermatologic Treatments and Research
- Fungal Infections and Studies
- Skin and Cellular Biology Research
- T-cell and B-cell Immunology
- Eosinophilic Disorders and Syndromes
- Poxvirus research and outbreaks
- Atherosclerosis and Cardiovascular Diseases
- Cell Adhesion Molecules Research
University of Lübeck
2014-2024
IL-17A has been identified as key regulatory molecule in several autoimmune and chronic inflammatory diseases followed by the successful use of anti-IL-17 therapy, e.g. ankylosing spondylitis psoriasis. Bullous pemphigoid (BP) is most frequent blistering disease with a high need for more specific, effective safe treatment options. The aim this study was to clarify pathophysiological importance BP. We found elevated numbers IL-17A+ CD4+ lymphocytes peripheral blood BP patients CD3+ cells...
Antilaminin 332 mucous membrane pemphigoid (MMP) is an autoimmune subepidermal blistering disease with predominant mucosal involvement and autoantibodies against laminin 332. Malignancies have been associated this disease; however, no standardized detection system for antilaminin serum antibodies widely available.Development of a sensitive specific assay the antibodies.An indirect immunofluorescence (IF) using recombinant was developed probed large number MMP patient sera (n = 93), as well...
Background Anti-p200 pemphigoid is a subepidermal autoimmune blistering disease (AIBD) characterized by autoantibodies against 200 kDa protein. Laminin γ1 has been described as target antigen in 70-90% of patients. No diagnostic assay widely available for anti-p200 pemphigoid, which might be due to the unclear pathogenic relevance anti-laminin autoantibodies. Objective To identify with higher clinical and relevance. Methods Immunoprecipitation, mass spectrometry, immunoblotting were employed...
Mucous membrane pemphigoid is an autoantibody-mediated disease predominantly affecting the oral cavity, pharynx, and conjunctiva. Conjunctival lesions may lead to impaired vision and, finally, blindness. About 25% of mucous patients generate autoantibodies against α3 chain laminin 332 (LAMα3), a structural protein epidermal/epithelial basement membranes. Here, we established mouse model by passive transfer rabbit IgG murine homologs two immunodominant fragments in adult C57BL/6 mice...
Bullous pemphigoid (BP) is an autoimmune blistering disease characterized by autoantibodies targeting type XVII collagen (Col17) with the noncollagenous 16A (NC16A) ectodomain representing immunodominant site. The role of additional extracellular targets Col17 outside NC16A has not been unequivocally demonstrated. In this study, we showed that ectodomain-reactive patient sera depleted in IgG induced dermal-epidermal separation a cryosection model indicating pathogenic potential anti-Col17...
Bullous pemphigoid (BP) is a subepidermal blistering disease characterized by autoantibodies against the two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. The multicentre prospective BLISTER (Bullous Pemphigoid Steroids Tetracyclines) trial randomized 253 patients with BP to compare benefits harms between initial treatment doxycycline or prednisolone.To analyse distinct autoantibody profiles for prediction of course in well-characterized cohort sera.One hundred forty-three...
Because of the morbidity and limited therapeutic options autoimmune diseases, there is a high, thus far, unmet medical need for development novel treatments. Pemphigoid such as epidermolysis bullosa acquisita (EBA), are prototypical diseases that caused by autoantibodies targeting structural proteins skin, leading to inflammation, mediated myeloid cells. To identify treatment targets, we performed cutaneous genome-wide mRNA expression profiling in 190 outbred mice after EBA induction....
Pemphigus vulgaris (PV) is a potentially life-threatening autoimmune blistering disease which associated with autoantibodies directed against two desmosomal proteins, desmoglein (Dsg) 3 and 1. Treatment of PV rather challenging relies on the long-term use systemic corticosteroids additional immunosuppressants. More recently, autoantibody-depleting therapies such as rituximab, high-dose intravenous immunoglobulins, immunoadsorption were shown to be valuable treatment options in PV. Specific...
retrospective design, use of claims data, and lack correlation with clinical outcomes.
Anti-laminin 332 mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by predominant mucosal lesions and autoantibodies against laminin 332. The exact diagnosis of anti-laminin MMP important since nearly 30% patients develop solid cancers. This study compared two independently developed diagnostic indirect immunofluorescence (IF) tests based on recombinant expressed in HEK239 cells (biochip mosaic assay) the migration trails cultured keratinocytes rich...
<h3>Importance</h3> Ecthyma contagiosum, or orf, is a viral zoonotic infection caused by<i>Poxviridae</i>. Although human orf considered to follow self-limited course, various immunological reactions may be triggered, including immunobullous diseases. In the majority of latter cases, antigenic target remained enigmatic. <h3>Objective</h3> To characterize predominant autoantigen in orf-induced disease and further describe this clinical entity. <h3>Design, Setting, Participants</h3> This...
Abstract The 16th non‐collagenous domain (NC16A) of BP180 is the main antigenic target autoantibodies in bullous pemphigoid (BP) and mucous membrane (MMP). Commercially available assays detect serum against NC16A majority BP (80%–90%) approximately 50% MMP patients. However, a standardized test system for detecting antibodies other regions still lacking. Moreover, anti‐BP180 have been found neurological conditions such as multiple sclerosis Parkinson disease. This study aimed at identifying...
Chronic blistering at the skin and/or mucous membranes, accompanied by a varying degree of inflammation, is clinical hallmark pemphigoid diseases that impose major medical burden. Pemphigoid are caused autoantibodies targeting structural proteins epithelial basement membrane. One pathogenic pathway and inflammation activation myeloid cells following Fc gamma receptor-dependent binding to skin-bound immune complexes. This process requires specific kinases, such as PI3Kδ, which have emerged...