The cell wall integrity mitogen-activated protein kinase (MAPK) cascade of Saccharomyces cerevisiae drives changes in gene expression response to stress. We show that the MAPK this pathway (Mpk1) and its pseudokinase paralog (Mlp1) use a noncatalytic mechanism activate transcription FKS2 gene. Transcriptional activation was dependent on Swi4/Swi6 (SBF) factor an activating signal Mpk1 but not activity. Activated (phosphorylated) Mlp1 were detected complex with Swi4 Swi6 at promoter....

AIM:To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus ) water extract on human hepatoma cell lines, HepG2 Hep3B cells. METHODS: The cytotoxicity was s c r e n d b y 3 -[ 4 , 5 -d i m t h l a z o -2 -y ] p u ( M TT y.Morphological observation, flow cytometry analysis, Western blot were employed to elucidate cytotoxic mechanism extract.RESULTS: cells more sensitive than cells, as demonstrated by markedly reduced viability.Chaga inhibited growth in...

Background/Objectives: Although donepezil, a reversible acetylcholinesterase inhibitor, has been in use since 1996, its metabolic characteristics remain poorly characterized. Therefore, this study aims to investigate the vivo metabolism of donepezil using liquid chromatography–high-resolution mass spectrometry (LC-HRMS) based on molecular networking (MN) approach integrated with non-targeted metabolomics approach. Methods: After oral administration (30 mg/kg) rats, urine, feces, and liver...

Eok-Keun Ahn, Kyung-Ho Kang, Hyang-Mi Park, Yong-Jae Won, Kuk-Hyun Jung, Woong-Jo Hyun, Yoon-Sung Lee, Ki-Young Kim, Mi-Jung Ji-Eun Kwak, Sang-Beom and Kyeong-Hee Jang. Korean J. Breed. Sci. 2025;57:29-38. https://doi.org/10.9787/KJBS.2025.57.1.29

The yeast SBF transcription factor is a heterodimer comprised of Swi4 and Swi6 that has well defined role in cell cycle-specific transcription. serves second function the transcriptional response to wall stress which activated Mpk1 mitogen-activated protein kinase integrity signaling pathway forms complex with Swi4, DNA binding subunit SBF, conferring upon ability bind activate FKS2. Although Mpk1–Swi4 formation activation FKS2 does not require catalytic activity, phosphorylated by must be...

Abstract To improve understanding of the characteristics large‐scale environments associated with summer monsoon precipitation in Korea, we have constructed an index to measure Changma using area‐averaged and period‐averaged Climate Prediction Center merged analysis over Korea during years 1979–2001. Strong weak are defined as which anomaly is ±0.5σ. The domain period for this selected Korean peninsula (31.25–41.25 °N, 123.75–131.25 °E) from late June July. In strong years, upper level...

The Mpk1 mitogen-activated protein kinase (MAPK) of the cell wall integrity signaling pathway uses a noncatalytic mechanism to activate SBF (Swi4/Swi6) transcription factor. Active forms complex with Swi4, DNA-binding subunit SBF, conferring ability bind DNA. Because activation is independent catalytic activity but requires be in an active conformation, we sought understand how interacts Swi4. Mutational analysis revealed that binding and Swi4 by intact D-motif-binding site, docking surface...

Even though Candida albicans commonly colonizes on most mucosal surfaces including the vaginal and gastrointestinal tract, it can cause candidiasis as an opportunistic infectious fungus. The emergence of resistant strains toxicity anti-fungal agents have encouraged development new classes potential agents. Sclareol, a labdane-type diterpene, showed anti-Candida activity with minimum inhibitory concentration 50 μg/mL in 24 h based microdilution susceptibility test. Cell membrane permeability...

This study investigated the effects of fucoidan (extract from Hizikia fusiforme) on symptoms and inflammatory cytokine activation in rats with monosodium iodoacetate (MIA)-induced osteoarthritis (OA). Forty male SD were divided into five groups, including normal, negative control (MIA), positive (Lyprinol), two experimental groups treated 50 or 100 mg/kg fucoidan. Weight-bearing assessments done after MIA injection right knee to induce OA. After 14 days treatment, microcomputed tomographic...

Two Gram-stain-negative, yellow, aerobic and rod-shaped bacterial isolates, designated THG-DN3.18(T) THG-DN3.19(T), were isolated from an ancient tree trunk Daejeon, South Korea. 16S rRNA gene sequence similarity showed that both strains belong to the genus Pedobacter within family Sphingobacteriaceae . Strain exhibited maximum with boryungensis KCTC 23344(T) (98.5%) while strain THG-DN3.19(T) nyackensis LMG 24260(T) (97.3%). In DNA-DNA hybridization tests, two less than 35% relatedness...

Salt sensitivity of blood pressure is characterized by inappropriate sympathoexcitation and renal Na+ reabsorption during high salt intake. In salt-resistant animal models, exogenous norepinephrine (NE) infusion promotes salt-sensitive hypertension prevents dietary Na+-evoked suppression the Na+-Cl- cotransporter (NCC). Studies adrenergic signaling pathways that modulate NCC activity NE have yielded conflicting results implicating α1- and/or β-adrenoceptors a downstream kinase network...

Keratinocyte proliferation is important for skin wound healing.The healing process includes blood clotting around the wound, removal of dead cells and pathogens through inflammation, then re-epithelialization maturation.Proliferation assay was performed on acid natural compounds to identify candidates natural-derived components injury treatment.We found that gentisic promoted high cell activity compared with other compounds.Gentisic improved HaCaT by over 20% in MTT assay.Gentisic also had...

Injection inoculation protocols for fruit body formation of Cordyceps militaris (C. militaris) were investigated to improve the incidence infection in silkworm species Bombyx mori (B. mori). Injection, with suspensions C. hyphal bodies into living pupae, was used test production. Use Daeseungjam rather than Baegokjam or Keumokjam varieties B. is thought be suitable by militaris. From mounting, nine-day-old 11-day-old pupae showed best a 100 µL injection volume. Silkworm injected suspension...

Sakuranetin is flavonoid phytoalexin that serves as a plant antibiotic and exists in Prunus several other species. Recently, we identified the anti-inflammatory effect of yedoensis found there were few studies on potential activity sakuranetin, one main constituents yedoensis. Here, isolated peritoneal macrophages from thioglycollate-injected mice examined whether sakuranetin affected response to lipopolysaccharide (LPS) plus interferon- (IFN-) γ or LPS only. suppressed synthesis iNOS COX2...

The proliferation of keratinocytes is one the important steps in wound-healing process, which regulated by various signals. Prior studies have shown that Trichosanthes kirilowii extract has ability to promote angiogenesis. Therefore, this study, we tested efficacy with respect promoting keratinocyte proliferation. A total 100 μg/mL treatment improved 145.38% compared DMSO-treated control an MTT assay and increased 238.2% wound closure re-epithelialization vitro assay. promoted ERK1/2...

Carotid artery intima-media thickness (CIMT) has recently been recommended as a non-invasive tool for primary prevention of cardiovascular events; the association between CIMT and adverse events is well-known. We sought to evaluate prevalence significance carotid plaque, especially in patients with coronary atherosclerosis.The study population consisted 1,705 consecutive {933 males (54.7%); mean age, 59.7+/-10.9 years} who underwent angiography scanning using high-resolution ultrasonography....

The Mpk1 MAP kinase of the cell wall integrity (CWI) signalling pathway induces transcription FKS2 gene in response to stress through a non-catalytic mechanism that involves stable association with Swi4 factor. This dimeric complex binds recognition site promoter. Swi6 factor is also required bind this ternary for initiation ensue. In context, Mlp1 pseudokinase serves redundant function Mpk1. We have identified three additional genes, CHA1, YLR042c and YKR013w, are induced by same mechanism....