A5075847873- Protease and Inhibitor Mechanisms
- Liver physiology and pathology
- Parathyroid Disorders and Treatments
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Liver Disease Diagnosis and Treatment
- Phytochemistry and Bioactive Compounds
- Macrophage Migration Inhibitory Factor
- Plant-derived Lignans Synthesis and Bioactivity
- Connective tissue disorders research
- Extracellular vesicles in disease
- Dementia and Cognitive Impairment Research
- Aortic aneurysm repair treatments
- Organ Transplantation Techniques and Outcomes
- Cancer, Hypoxia, and Metabolism
- Inflammatory Bowel Disease
- PI3K/AKT/mTOR signaling in cancer
- Wnt/β-catenin signaling in development and cancer
- Galectins and Cancer Biology
- Bone and Dental Protein Studies
- Traditional and Medicinal Uses of Annonaceae
- Blood properties and coagulation
- Caveolin-1 and cellular processes
- Ginger and Zingiberaceae research
- Iron Metabolism and Disorders
Charité - Universitätsmedizin Berlin
2015-2025
Humboldt-Universität zu Berlin
2017-2025
Freie Universität Berlin
2017-2025
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020
Franklin University
2005-2011
University of Bern
2011
University of Padua
2006
Royal London Hospital
2006
Harvard University
2006
Hammersmith Hospital
2006
The matrix metalloproteinases (MMP) MMP-2 and MMP-9 are physiological regulators of vascular remodelling. Their dysregulation could contribute to calcification. We examined the role in uraemic calcification vivo vitro.The impact pharmacological MMP inhibition on development media calcifications was explored an aggressive animal model In addition, selective effects addition inhibition, respectively, calcium-/phosphate-induced were studied a murine cell line smooth muscle cells...
Chronic kidney disease (CKD) is associated with excessive mortality from cardiovascular (CVD). Endothelial dysfunction, an early manifestation of CVD, consistently observed in CKD patients and might be linked to structural defects the microcirculation including microvascular rarefaction. However, patterns rarefaction their relation functional deficits perfusion oxygen delivery are currently unknown. In this in-vivo microscopy study cremaster muscle BALB/c mice moderate severe uremia, we show...
Abstract Background The field of application organic or inorganic nanoparticles (1-100 nm size) is extensive and involves their use in medicine. This includes using human beings animals (life-stock) which holds advantages but also bears risks. Therefore, used diagnostics therapy should be inert not affecting tissues immune cells. Additionally, aggregate to form clusters exceeding the size 100 accumulate sensitive structures like bone marrow brain for long-term deposition. We developed...
Gut-derived uremic toxins (UTs) contribute to cardiovascular disorders like atherosclerosis and cardiomyopathy in patients with chronic kidney disease (CKD), causing increased morbidity mortality. The intermediate steps between higher concentrations of gut-derived UTs organ damage caused by are still insufficiently understood. Glycosaminoglycans (GAGs) as components the extracellular matrix known interact various ligands such growth factors or receptors, thereby influencing...
Vascular calcifications such as arteriosclerosis, which is characterized by a calcificiation of the tunica media, represent major comorbidities e.g. in patients with chronic kidney disease (CKD). An essential step during development arteriosclerosis transdifferentiation/calcification vascular smooth muscle cells (VSMC) resembling osteogenesis. The matrix metalloproteinases (MMP)-2 and −9 were shown to promote these VSMC their inhibition therapeutic value prevent preclinical studies. Aiming...
Fibrolytic and profibrotic activities of the matrix metalloproteinases (MMPs)-2 -9 play a central role in liver fibrosis. Since binding to extracellular influences activity both gelatinases, here fibrillar collagens as most abundant components fibrotic tissue was investigated.In situ zymography immunohistology showed association enzymatically inactive prodomain-containing proMMP-2 proMMP-9 but not their activated forms collagen structures, which are substrates these gelatinases. In...
In liver fibrosis, activated hepatic stellate cells (HSC) secrete excess extracellular matrix, thus, represent key targets for antifibrotic treatment strategies. Intermediate-conductance Ca(2) (+) -activated K(+) -channels (KCa3.1) are expressed in non-excitable tissues affecting proliferation, migration and vascular resistance rendering KCa3.1 potential fibrosis. So far, no information about expression their role HSC exists. Aim was to quantify the depending on activation investigation of...
Abstract Extracellular vesicles (EV) function as messengers between endothelial cells (EC) and vascular smooth muscle (VSMC). Since chronic kidney disease (CKD) increases the risk for calcifications, we investigated whether EV derived from uraemic milieu‐stimulated EC rats impact osteogenic transdifferentiation/calcification of VSMC. For that purpose, human were treated with urea indoxyl sulphate or left untreated. Experimental uraemia in was induced by adenine feeding. ‘Uraemic’ control (EV...
Abstract Background In traditional Chinese and Korean medicine, an aqueous extract derived from wood bark of the Japanese spice bush Lindera obtusiloba ( L.obtusiloba ) is applied to treat inflammations chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic on human carcinoma (HCC) cell lines signaling pathways involved. Methods Four HCC representing diverse...
Abstract Aim Activation of vascular smooth muscle cells ( VSMC ), a key event in the pathogenesis atherosclerosis, is triggered by inflammatory stimuli such as tumour necrosis factor‐alpha TNF ‐ α ) causing mitogenic response. The polyphenol (+)‐episesamin ES was shown to counteract ‐induced effects, for example macrophages. Aiming novel therapeutic options, we here investigated whether protects from growth and migration, which both contribute onset progression atherosclerosis. Methods Human...
Collagen XIV (CXIV) is a fibril-associated collagen that mainly expressed in well differentiated tissues and late embryonic development. Because CXIV almost absent proliferating and/or dedifferentiated tissues, functional role maintaining cell differentiation suspected. We demonstrate antiproliferative, quiescence- differentiation-inducing effects of human its recombinant fragments on mesenchymal cells. In primary fibroblasts, mouse 3T3 fibroblasts 3T3-L1 preadipocytes, reduced de novo DNA...
Inflammatory bowel diseases (IBD) comprise mainly ulcerative colitis (UC) and Crohn´s disease (CD). Both forms present with a chronic inflammation of the (gastro) intestinal tract, which induces excessive changes in composition associated extracellular matrix (ECM). In UC, is limited to colon, whereas it can occur throughout entire gastrointestinal tract CD. Tools for early diagnosis IBD are still very highly invasive measures standardized evaluation structural scarce. To investigate an...
Abstract Background Agitation is common in geriatric patients with cognitive impairment, e.g. persons dementia (PWD), who are admitted to an emergency department (ED). It might be a first sign of upcoming delirium and associated higher risk unfavorable clinical course. Hence, monitoring vital signs enhanced movement as indicators agitation essential these during their stay the ED. Since PWD rarely tolerate fixed devices, novel developed non-contact system (NCMSys) represent appropriate...
Endothelial dysfunction is an early manifestation of cardiovascular disease (CVD) and consistently observed in patients with chronic kidney (CKD). We hypothesized that CKD associated systemic damage to the microcirculation, preceding macrovascular pathology. To assess degree "uremic microangiopathy", we have measured microvascular density biopsies omentum children CKD.Omental tissue was collected from 32 healthy (0-18 years) undergoing elective abdominal surgery 23 age-matched cases stage 5...
Abstract Chronic kidney disease (CKD) greatly increases the risk for cardiovascular (CVD). However, molecular mechanisms underlying CKD-induced arterial remodeling are largely unknown. We performed a systematic analysis of biopsies from children with stage 5 predialysis CKD participating in Cardiovascular Comorbidity Children Kidney Disease (4 C) study. For comparison, we studied without CKD, coronary bypass vessels adults atherosclerotic heart and aortic sections subtotally nephrectomized...
Uremic toxins exert pathophysiological effects on cells and tissues, such as the generation of a pro-calcifying subtype exosome-like extracellular vesicles (EVs) in vascular cells. Little is known about surface structure EVs. Thus, we studied uremic abundance sulfated glycosaminoglycans (GAGs) EVs, implications for binding ligands very small superparamagnetic iron oxide particles (VSOPs) which could be relevance radiological EV-imaging. Vascular were treated with NaH2PO4 mixture urea indoxyl...