A5023494416- Analytical chemistry methods development
- Ion-surface interactions and analysis
- Laser-induced spectroscopy and plasma
- Mass Spectrometry Techniques and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Mercury impact and mitigation studies
- Nuclear Physics and Applications
- Biosensors and Analytical Detection
- Prostate Cancer Treatment and Research
- Heavy metals in environment
- Nanocluster Synthesis and Applications
- Trace Elements in Health
- Nanoparticles: synthesis and applications
- Nanoparticle-Based Drug Delivery
- Effects and risks of endocrine disrupting chemicals
- Metabolomics and Mass Spectrometry Studies
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Conservation Techniques and Studies
- Recycling and Waste Management Techniques
- Protease and Inhibitor Mechanisms
- Scientific Measurement and Uncertainty Evaluation
- Microscopic Colitis
- Cell Adhesion Molecules Research
Federal Institute For Materials Research and Testing
2013-2025
We have applied a micro droplet generator (μDG) for sample introduction of single selenized yeast cells into sector field ICP-MS, which was operated in fast scanning mode with sampling rates up to 10 kHz, measure time resolved 100 μs integration time. Selenized been used as model system preliminary investigation. The be measured embedded droplets and it will shown that the duration cell event always is about 400 500 μs, thus comparable without cell. A fixed generation rate 50 Hz produced...
A micro-droplet generator (μDG) sample introduction system was coupled to a sector field ICP-MS instrument investigate the analytical figures of merit with respect single cell analysis. The operated for first time in fast scanning mode (E-scan) shortest resolution 100 μs measure droplet resolved and using original detector pulse counting without modification existing electronics. For reduction diameter triple applied down 23 μm has been achieved this investigation 100% transport efficiency...
The analytical potential of a nanosecond laser ablation inductively coupled plasma mass spectrometer system, equipped with an ultra-fast wash-out chamber, is critically investigated for fast and highly spatially resolved (∼μm) qualitative elemental distribution within single cells.
Stable metal isotopes have received increasing attention as medical biomarkers due to their potential detect changes in metabolism related diseases. In particular, copper stable are a powerful tool identify isotopic variation between tumors and healthy tissue, suggesting application cancer diagnosis. However, mechanisms causing isotope fractionation, such redox- or bond-forming reactions interactions of metals during transmembrane import export, less well understood. Here, we established an...
By adding a gold core to silica nanoparticles (BrightSilica), silica‐like are generated that, unlike unmodified nanoparticles, provide three types of complementary information investigate the nano‐biointeraction inside eukaryotic cells in situ. Firstly, organic molecules proximity and penetrating into shell live monitored by surface‐enhanced Raman scattering (SERS). The SERS data show interaction hybrid particles with tyrosine, cysteine phenylalanine side chains adsorbed proteins....
A comparison of single cell LA-ICP-MS imaging and spot analysis highlights the advantages both methods.
The nano-bio interaction and physicochemical properties of plasmonic non-plasmonic gold nanostructures grown inside cells vary depending on incubation conditions.
We applied high resolution laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOF-MS) with cellular spatial for bioimaging of nanoparticles uptaken by fibroblast multicellular spheroids (MCS). This was used to quantitatively investigate interactions silver (Ag NPs) and the distributions intrinsic minerals biologically relevant elements within thin sections a MCS as three-dimensional in vitro tissue model. designed matrix-matched calibration standards this...
Prostate cancer (PCa) is one of the most common cancers in men. For detection and diagnosis PCa, non-invasive methods, including magnetic resonance imaging (MRI), can reduce risk potential surgical intervention. To explore molecular characteristics tumor, we investigated applicability ferumoxytol PCa a xenograft mouse model two different tumor volumes, 500 mm3 1000 mm3. Macrophages play key role progression, they are able to internalize iron-oxide particles, such as ferumoxytol. When...
Multifunctional composite nanoprobes, Ag–Magnetite and Au–Magnetite, were manipulated in fibroblast cells characterized using SERS, LA-ICP-MS, nanotomography.
Directing nanoparticles to the nucleus by attachment of nuclear localization sequences (NLS) is an aim in many applications. Gold modified with two different NLS were studied while crossing barriers intact cells, including uptake, endosomal escape, and translocation. By imaging characterization their molecular interactions surface-enhanced Raman scattering (SERS), it shown that translocation strongly depends on particular incubation conditions. After 1 h followed a 24 chase time, 14 nm gold...
Using a murine model of multiple sclerosis, we previously showed that repeated administration gadopentetate dimeglumine led to retention gadolinium (Gd) within cerebellar structures and this process was enhanced with inflammation. This study aimed compare the kinetics profiles Gd in inflamed healthy brains after application macrocyclic Gd-based contrast agent (GBCA) gadobutrol or linear GBCA gadopentetate. Moreover, potential Gd-induced neurotoxicity investigated living hippocampal slices ex...
To test whether the silicate reference glasses BAM‐S005‐A and BAM‐S005‐B from BAM (The Federal Institute for Materials Research Testing, Germany) are suitable materials microanalysis, we investigated homogeneity of these using microanalytical techniques EPMA, LA‐ICP‐MS SIMS. Our study indicated that all major most trace elements homogeneously distributed at micrometre sampling scale in both types glass. However, some (e.g., Cs, Cl, Cr, Mo Ni) seem to be inhomogeneously distributed. We also...
Inflammatory bowel diseases (IBD) comprise mainly ulcerative colitis (UC) and Crohn´s disease (CD). Both forms present with a chronic inflammation of the (gastro) intestinal tract, which induces excessive changes in composition associated extracellular matrix (ECM). In UC, is limited to colon, whereas it can occur throughout entire gastrointestinal tract CD. Tools for early diagnosis IBD are still very highly invasive measures standardized evaluation structural scarce. To investigate an...
Abstract The incidence of abdominal aortic aneurysms (AAAs) has substantially increased during the last 20 years and their rupture remains third most common cause sudden death in cardiovascular field after myocardial infarction stroke. only established clinical parameter to assess AAAs is based on aneurysm size. Novel biomarkers are needed improve assessment risk rupture. ADAMTS4 (A Disintegrin And Metalloproteinase with ThromboSpondin motifs 4) a strongly upregulated proteoglycan cleaving...
In this work, different calibration strategies for the determination of trace elements in pure copper metal by nanosecond laser ablation ICP-MS were investigated. addition to certified reference materials (CRMs), pellets doped powder used calibration. The micro homogeneity CRMs as well solution-doped was sufficient use them samples combination with a spot size 200 μm. contrast, analytes solid form showed significant heterogeneity. For most investigated and measured mass fractions within ±...
Three methods for direct solid sampling of bulk material namely IR laser ablation, glow discharge and spark OES, were compared with respect to analytical figures merit obtained elemental analysis atomic spectrometry. Matrices investigated copper, pressed doped copper powder, magnesium alloys. For the vast majority analytes, statistical equivalence regarding precision (usually ≤5%) performance calibrations between was demonstrated.