A5102721367- Dialysis and Renal Disease Management
- Central Venous Catheters and Hemodialysis
- Muscle and Compartmental Disorders
- Liver Disease and Transplantation
- Chronic Kidney Disease and Diabetes
- Complement system in diseases
- Renal function and acid-base balance
- Peripheral Artery Disease Management
- Clinical Nutrition and Gastroenterology
- Electrolyte and hormonal disorders
- Parathyroid Disorders and Treatments
- Organ Transplantation Techniques and Outcomes
- Pharmacological Effects and Toxicity Studies
- Renal and Vascular Pathologies
- Potassium and Related Disorders
- Hemoglobinopathies and Related Disorders
- Acute Kidney Injury Research
- Nutrition and Health in Aging
- Lymphatic Disorders and Treatments
- Extracellular vesicles in disease
- Membrane Separation Technologies
- Magnesium in Health and Disease
- Pharmaceutical studies and practices
- MicroRNA in disease regulation
- Neurological Complications and Syndromes
Heidelberg University
2014-2023
University Hospital Heidelberg
2012-2023
German Center for Pediatric and Adolescent Rheumatology
2011-2021
Boston Children's Hospital
2013
Abstract The peritoneum plays an essential role in preventing abdominal frictions and adhesions can be utilized as a dialysis membrane. Its physiological ultrastructure, however, has not yet been studied systematically. 106 standardized peritoneal 69 omental specimens were obtained from 107 patients (0.1–60 years) undergoing surgery for disease affecting the automated quantitative histomorphometry immunohistochemistry. mesothelial cell layer morphology protein expression pattern is similar...
Background. Molecular Adsorbents Recirculating System (MARS) is an extracorporeal liver support system eliminating albumin-bound and water-soluble substances. While it increasingly applied in patients with acute failure (ALF), no comparison standard dialysis methods has yet been performed. Methods. This analysis of ten children (0.1–18 years) ALF, who underwent a total 22 MARS sessions. Standard adult sets were used seven (23.5–72 kg) Mini three (2.8–13 kg). In eight children, was alternated...
Cardiovascular disease (CVD) is the leading cause of increased mortality in patients with CKD and further aggravated by peritoneal dialysis (PD). Children are devoid preexisting CVD provide unique insight into specific uremia- PD-induced pathomechanisms CVD. We obtained specimens from children stage 5 at time PD catheter insertion (CKD5 group), established (PD age-matched nonuremic controls (n=6/group). microdissected omental arterioles tissue layers not directly exposed to fluid used...
The impact of peritoneal dialysis (PD) associated peritonitis on membrane integrity is incompletely understood. Children are particularly suited to address this question, since they largely devoid preexisting tissue damage and life-style related alterations. Within the International Peritoneal Biobank, 85 standardized parietal samples were obtained from 82 children neutral pH PD fluids with low glucose degradation product (GDP) content. 37 patients had a history 16 two or more episodes. Time...
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Consideration of specific pediatric aspects is essential to achieve adequate peritoneal dialysis (PD) treatment in children. These are first all the rapid growth, particular during infancy and puberty, which must be accompanied by a positive calcium balance, age dependent changes body composition. The high total water content ultrafiltration rates required anuric infants for nutrition predispose overshooting convective sodium losses severe hypotension. Tissue fragility increases...
Long-term clinical outcome of peritoneal dialysis (PD) depends on adequate removal small solutes and water. The endothelium represents the key barrier transport dysfunction is associated with vascular changes. Alanyl-glutamine (AlaGln) has been shown to counteract PD-induced deteriorations but effect changes not yet elucidated. Using multiplexed proteomic bioinformatic analyses we investigated molecular mechanisms pathology in-vitro (primary human umbilical vein endothelial cells, HUVEC)...
Hyperparathyroidism (HPT) is an essential contributor to bone disease and cardiovascular calcifications in children with chronic kidney (CKD). Pharmacological dietary interventions are of limited efficacy; calcimimetics not yet recommended children. Parathyroidectomy (PTX) ultimately performed if HPT becomes refractory conservative measures; the long-term results impact subsequent transplantation (NTX), however, have been evaluated.We analyzed postsurgical course 18 paediatric CKD patients...
Patients with immune-mediated kidney disease and liver failure often require plasma exchange (PE) hemodialysis (HD). Combining both methods (i.e., connecting the PE HD circuits in series [tandem dialysis]) should allow for a more efficient treatment. This work reviews authors' experience tandem blood purification.Chart review was utilized to retrospectively analyze efficacy tolerability of 92 combined PE/HD (cPE/HD) sessions 26 children comparison 113 sequential (sPE/HD) treatments performed...
Endothelial dysfunction is an early manifestation of cardiovascular disease (CVD) and consistently observed in patients with chronic kidney (CKD). We hypothesized that CKD associated systemic damage to the microcirculation, preceding macrovascular pathology. To assess degree "uremic microangiopathy", we have measured microvascular density biopsies omentum children CKD.Omental tissue was collected from 32 healthy (0-18 years) undergoing elective abdominal surgery 23 age-matched cases stage 5...
Ultrafiltration decline is a progressive issue for patients on chronic peritoneal dialysis (PD) and can be caused by angiogenesis induced PD fluids. A recent pediatric trial suggests better preservation of ultrafiltration with bicarbonate versus lactate buffered fluid; underlying molecular mechanisms are unknown.Angiogenic cytokine profile, tube formation capacity Receptor Tyrosine Kinase translocation were assessed in primary human umbilical vein endothelial cells following incubation...
Chronic kidney disease (CKD) frequently leads to hyperphosphatemia and hyperparathyroidism, mineral bone disorder (CKD-MBD), ectopic calcifications cardiovascular mortality. PTH activates the osteoanabolic Gαs/PKA Gαq/11/PKC pathways in osteoblasts, specific impact of latter CKD-MBD is unknown. We generated osteoblast Gαq/11 knockout (KO) mice established by subtotal nephrectomy dietary phosphate load. Bone morphology was assessed micro-CT, function planar scintigraphy at week 10 22...