A5013041465- Dialysis and Renal Disease Management
- Iron Metabolism and Disorders
- Nutrition and Health in Aging
- Chronic Kidney Disease and Diabetes
- Occupational and environmental lung diseases
- Metabolism and Genetic Disorders
- Spaceflight effects on biology
- Peripheral Artery Disease Management
- Erythropoietin and Anemia Treatment
- Tissue Engineering and Regenerative Medicine
- Fungal and yeast genetics research
- Bioinformatics and Genomic Networks
- Polysaccharides and Plant Cell Walls
- Bone Metabolism and Diseases
- Advanced Proteomics Techniques and Applications
- Cystic Fibrosis Research Advances
- Amoebic Infections and Treatments
- Ergonomics and Musculoskeletal Disorders
- Parathyroid Disorders and Treatments
- Prostate Cancer Treatment and Research
- Folate and B Vitamins Research
- Periodontal Regeneration and Treatments
- Effects of Vibration on Health
- Skin and Cellular Biology Research
- Advanced Glycation End Products research
Medical University of Vienna
2016-2025
Christian Doppler Laboratory for Thermoelectricity
2016-2024
Ludwig-Maximilians-Universität München
2021
Comprehensive Cancer Center Vienna
2020
Basys (Germany)
2017
The microaerophilic/anaerobic protist Giardia lamblia is a world-wide occurring parasite of the human small intestine. It causes giardiasis which manifests as diarrhoea accompanied by other sequelae. Giardiasis most commonly treated with either 5-nitroimidazole metronidazole or benzimidazole albendazole. Unfortunately, number refractory cases increasing, probably caused, at least in part, drug resistance. However, attempts to isolate metronidazole-resistant G. strains from patients have...
Abstract Duchenne muscular dystrophy (DMD), a severe muscle disease caused by mutations in the gene encoding for intracellular protein dystrophin, is associated with impaired cardiac function and arrhythmias. A causative factor complications dystrophic heart abnormal calcium (Ca) handling ventricular cardiomyocytes, restoration of normal Ca homeostasis has emerged as therapeutic strategy. Here, we used rodent model DMD, dystrophin-deficient DMD mdx rat, to test following hypothesis: chronic...
Lithium added to peritoneal dialysis fluid protects mesothelial cells and attenuates fibrosis angiogenesis by reducing αB-crystallin.
Plectin, a highly versatile and multifunctional cytolinker, has been implicated in several multisystemic disorders. Most sequence variations the human plectin gene (PLEC) cause epidermolysis bullosa simplex with muscular dystrophy (EBS-MD), an autosomal recessive skin-blistering disorder associated progressive muscle weakness. In this study, we performed comprehensive cell biological analysis of dermal fibroblasts from three different patients EBS-MD, where PLEC expression analyses revealed...
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Long-term clinical outcome of peritoneal dialysis (PD) depends on adequate removal small solutes and water. The endothelium represents the key barrier transport dysfunction is associated with vascular changes. Alanyl-glutamine (AlaGln) has been shown to counteract PD-induced deteriorations but effect changes not yet elucidated. Using multiplexed proteomic bioinformatic analyses we investigated molecular mechanisms pathology in-vitro (primary human umbilical vein endothelial cells, HUVEC)...
Readily available peritoneal dialysis (PD) effluents from PD patients in the course of renal replacement therapy are a potentially rich source for molecular markers predicting clinical outcome, monitoring and therapeutic interventions. The complex phenotype might be reflected effluent metabolome. Metabolomic analysis allow quantitative detection assessment candidate biomarkers prognostication monitoring. We therefore subjected equilibration test 20 stable patients, obtained randomized...
Peritoneal dialysis (PD) is a modality of renal replacement therapy in which the high volumes available PD effluent (PDE) represents rich source biomarkers for monitoring disease and therapy. Although this information could help guide management patients, little known about potential PDE to define pathomechanism-associated molecular signatures PD.We therefore subjected high-performance multiplex proteomic analysis after depletion highly-abundant plasma proteins enrichment low-abundance...
To replace kidney function, peritoneal dialysis (PD) utilizes hyperosmotic PD fluids with specific physico-chemical properties. Their composition induces progressive damage of the peritoneum, leading to vasculopathies, decline membrane and technique failure. Clinically used differ in their but still remain bioincompatible. We mapped molecular pathomechanisms human endothelial cells induced by different characteristics widely proteomics. Of 7894 identified proteins, 3871 were regulated at...
Peritoneal dialysis (PD) fluids are cytotoxic to the peritoneum. Recent studies have shown that alanyl-glutamine (AlaGln) modulates cellular stress response, improves mesothelial cell survival, reduces submesothelial thickening in experimental models of PD, and clinical PD effluent immune responses. However, mechanisms AlaGln-mediated membrane protection not yet fully understood. Here, we explore those through application a novel proteomics approach clinically relevant vivo model rats....
Western blotting is widely used for protein identification and quantification in research applications, but different species, resulting from alternative splicing post-translational modifications, can often only be detected individually by two-dimensional gel electrophoresis immunodetection (2D-WB). The additional separation isoelectric focusing enables the detection of species with same specific antibody. Reliable assignment signals antibody-based to total spot pattern original image a...
Mass cytometry (MC) is a powerful method for mapping complex cellular systems at single-cell levels, based on the detection of proteins. Numerous studies have been performed using human blood, but there lack protocols describing processing and labeling bronchoalveolar lavage fluid (BALF) nasal polyps (NP) acquisition by MC. These specimens are essential in investigation immune cell characteristics airway diseases such as asthma chronic rhinosinusitis with NP (CRSwNP). Here we optimized...
Bone allografts are widely used as osteoconductive support to guide bone regrowth. more than a scaffold for the immigrating cells they maintain some bioactivity of original matrix. Yet, it remains unclear how respond allografts. To this end, we have evaluated response mesenchymal exposed acid lysates (ALBA). RNAseq revealed that ALBA has strong impact on genetic signature gingival fibroblasts, indicated by increased expression IL11, AREG, C11orf96, STC1, and GK—as confirmed RT-PCR, IL11 STC1...
<title>Abstract</title> Transforming growth factor β (TGF-β) is implicated in both mesothelial-to-mesenchymal transition (MMT) and cellular senescence of human peritoneal mesothelial cells (HPMCs). We previously showed that senescent HPMCs could spontaneously acquire some phenotypic features MMT, which young were induced by TGF-β. Here, we used electron microscopy, as well global gene protein profiling to assess detail how exposure TGF-β impacts on vitro. found structural changes consistent...
Abstract Background and Aims Transforming growth factor β1 (TGF-β1) is a recognized driver of both mesothelial-to-mesenchymal transition (MMT) cellular senescence observed in vitro human peritoneal mesothelial cells (HPMCs), have significant role adverse remodelling patients treated with dialysis (PD). We previously shown that senescent HPMCs spontaneously acquire some phenotypic features MMT which young are induced by TGF-β1. At the same time, appeared to be less responsive TGF-β. It not...
Abstract Transforming growth factor β (TGF-β) is implicated in both mesothelial-to-mesenchymal transition (MMT) and cellular senescence of human peritoneal mesothelial cells (HPMCs). We previously showed that senescent HPMCs could spontaneously acquire some phenotypic features MMT, which young were induced by TGF-β. Here, we used electron microscopy, as well global gene protein profiling to assess detail how exposure TGF-β impacts on vitro. found structural changes consistent with MMT young,...
Used hemodialysis membranes (HD-M) are a valuable reservoir of biological information. Proteins bind to HD-M, but whether this process depends on the type membrane or patient factors selectively affects specific protein classes has not been adequately elucidated. State-of-the-art proteomics techniques capable identifying and quantifying therapy-specific subproteome enable analysis disease- membrane-induced pathophysiologies. We demonstrate feasibility deep proteomic characterization...
Objective Subcutaneous adipose tissue (SAT) thickness above the anterior superior iliac spine (ASIS) influences belt fit of a vehicle occupant. To improve finite element (FE) human body models and their application assessing future seating positions in cars, there is need for more detailed data.Methods Anthropometric input data were used to statistically model lower limit SAT area around ASIS (at or groin) extracted from 102 postmortem computed tomography (pmCT) sets (56 males 46 females)....
is a soluble iron salt approved to maintain balance and hemoglobin in patients receiving chronic hemodialysis.FPC donates transferrin, bypassing the reticuloendothelial block metabolism.FPC added peritoneal dialysis fluid (PDF) may replace and/or stores.METHODS: Thirty (30) PD were enrolled an open-label, randomized, twoperiod, single ascending dose study of FPC administered PDF.All received either Dianeal V R (1.5% dextrose) 2 L or Extraneal (Icodextrin 7.5%), 1.5 for 12 hour dwell.FPC was...
Abstract Background and Aims PD-fluids lead to generation of reactive oxygen species (ROS) in the peritoneal cavity. The caused oxidative stress, defined as a cellular oxidant-antioxidant imbalance impairs not only cell viability but also contributes progression local systemic PD-related pathomechanisms. We aim analyze impact specific targets ROS during PD anti-oxidative mechanism supplementation PD-fluid with alanyl-glutamine (AlaGln) on global proteome-wide level. Method To establish...