A5031531338- Mast cells and histamine
- Proteoglycans and glycosaminoglycans research
- Advanced Breast Cancer Therapies
- Food Allergy and Anaphylaxis Research
- Ubiquitin and proteasome pathways
- Allergic Rhinitis and Sensitization
- Protein Degradation and Inhibitors
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- CRISPR and Genetic Engineering
- Chronic Lymphocytic Leukemia Research
- DNA Repair Mechanisms
- Eosinophilic Disorders and Syndromes
- Advanced Proteomics Techniques and Applications
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- Iron Metabolism and Disorders
- Dialysis and Renal Disease Management
- Enzyme Structure and Function
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Molecular Sensors and Ion Detection
- Cell death mechanisms and regulation
CeMM Research Center for Molecular Medicine
2013-2024
Austrian Academy of Sciences
2010-2024
Medical University of Vienna
2020
University of Vienna
2020
Tisch Hospital
2014
Tumor formation is a multistep process during which cells acquire genetic and epigenetic changes until they reach fully transformed state. We show that CDK6 contributes to tumor by regulating transcriptional responses in stage-specific manner. In early stages, the kinase induces complex program block p53 hematopoietic cells. Cells lacking function are required mutate TP53 (encoding p53) achieve immortalized binds promoters of genes including antagonists Prmt5, Ppm1d, Mdm4 The findings...
The complement system is a vital anti-microbial defence mechanism against circulating pathogens. Excessive activation can have deleterious outcomes for the host and consequently tightly modulated by set of membrane-associated fluid-phase regulators (RCAs). Here, we demonstrate that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hijacks cellular RCA members CD55 CD59 serum-derived Factor H (FH) to resist antibody-dependent complement-mediated lysis triggered immunised human...
In contrast to their clearly defined roles in allergic diseases, the physiologic functions of Immunoglobulin E antibodies (IgEs) and mast cells (MCs) remain enigmatic. Recent research supports toxin hypothesis, showing that MCs IgE-related type 2 immune responses can enhance host defense against certain noxious substances, including honeybee venom (BV). However, mechanisms by which interfere with BV toxicity are unknown. this study, we assessed role IgE MC products MC-mediated detoxification.
We provide a catalog for the effects of human kinome on cell survival in response to DNA-damaging agents, covering all major DNA repair pathways. By treating 313 kinase-deficient lines with ten diverse including seven commonly used chemotherapeutics, we identified examples vulnerability and resistance that are kinase specific. To investigate synthetic lethal interactions, tested carmustine 25 by establishing phenotypic fluorescence-activated sorting (FACS) assay designed validate gene-drug...
Solute carrier (SLC) transporters form a protein superfamily that enables transmembrane transport of diverse substrates including nutrients, ions and drugs. There are about 450 different SLCs, residing in variety subcellular membranes. Loss-of-function an unusually high proportion SLC is genetically associated with plethora human diseases, making SLCs rapidly emerging but challenging drug target class. Knowledge their environment may elucidate the molecular basis for functional integration...
Peritoneal dialysis (PD) is a modality of renal replacement therapy in which the high volumes available PD effluent (PDE) represents rich source biomarkers for monitoring disease and therapy. Although this information could help guide management patients, little known about potential PDE to define pathomechanism-associated molecular signatures PD.We therefore subjected high-performance multiplex proteomic analysis after depletion highly-abundant plasma proteins enrichment low-abundance...
Cyclin-dependent kinase 6 (CDK6) represents a novel therapeutic target for the treatment of certain subtypes acute myeloid leukaemia (AML). CDK4/6 inhibitors have been widely studied in many cancer types and their effects may be limited by primary secondary resistance mechanisms. degraders, which eliminate kinase-dependent kinase-independent effects, suggested as an alternative option. We show that efficacy CDK6-specific protein degrader BSJ-03-123 varies among AML depends on low expression...
In this study we show that via stable, retroviral-expression of tagged EGFR del (L747-S752 deletion mutant) in the PC9 lung cancer cell line and stable doxycycline-inducible expression Grb2 using a Flp-mediated recombination HEK293 system, SH-TAP can be downscaled to 5 12.5 mg total protein input (equivalent 0.5 - 1 × 15 cm culture plate or 4 8 106 cells). The major constituents complex (USB3B, GRB2, ERRFI, HSP7C, GRP78, HSP71) (ARHG5, SOS1, ARG35, CBL, CBLB, PTPRA, SOS2, DYN2, WIPF2, IRS4)...
Objective: Thrombus resolution is driven by leukocyte recruitment and thrombus angiogenesis. An effective inhibition of transmigration in vitro mediated naturally occurring peptide Bβ 15-42 , which a competitive inhibitor the interaction between N-terminus fibrin beta chain vascular endothelial cadherin. We investigated effect on murine stagnant flow venous thrombosis model studied levels human patients. Approach Results: 2 mouse models subtotal inferior vena cava ligation. In first model,...
OBJECTIVE: We investigated the role of glycosaminoglycan hyaluronan in experimental allergic encephalomyelitis (EAE). BACKGROUND: Hyaluronan may have proinflammatory roles context CNS autoimmunity: Hyaluronan accumulates demyelinated lesions, promotes antigen presentation and enhances T-cell activation proliferation. at vascular endothelium facilitate lymphocyte binding to vessels infiltration. Furthermore, signals through Toll-like receptor (TLR)-2 -4 stimulate inflammatory genes cells. ...
Background: Acute myeloid leukemia (AML) is a highly aggressive disease that comprises heterogeneous group of genetically distinct subtypes. AML initiated by leukemic cells, which are able to self-renew and give rise malignant blasts. A key regulator prognostic biomarker for the cell cycle Cyclin-dependent kinase 6 (CDK6) represents novel therapeutic target treatment certain subtypes AML. CDK4/6 inhibitors extensively studied in several cancer types but beneficial effects may be limited...