A5020233523- Drug Transport and Resistance Mechanisms
- Pharmacological Effects of Natural Compounds
- Ginseng Biological Effects and Applications
- Pharmacological Effects and Toxicity Studies
- Pharmacogenetics and Drug Metabolism
- Drug-Induced Hepatotoxicity and Protection
- Metabolism and Genetic Disorders
- Natural product bioactivities and synthesis
- Diabetes Treatment and Management
- Berberine and alkaloids research
- Drug Solubulity and Delivery Systems
- Antibiotics Pharmacokinetics and Efficacy
- Trace Elements in Health
- Metabolism, Diabetes, and Cancer
- Nanoparticle-Based Drug Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Sphingolipid Metabolism and Signaling
- Peptidase Inhibition and Analysis
- Silymarin and Mushroom Poisoning
- Food Quality and Safety Studies
- Pancreatic function and diabetes
- HIV/AIDS drug development and treatment
- Cancer therapeutics and mechanisms
- Advanced Drug Delivery Systems
- Phytochemistry and biological activity of medicinal plants
Kyungpook National University
2016-2025
Dankook University
2018
Inje University
2007-2015
Inje University Busan Paik Hospital
2012
Seoul National University
1999-2008
The University of Texas MD Anderson Cancer Center
2004-2008
National Cheng Kung University
2008
University Medical Center Utrecht
2008
San Francisco VA Medical Center
1992
Macropinocytosis, an important nutrient-scavenging pathway in certain cancer cells, allows cells to compensate for intracellular amino acid deficiency under nutrient-poor conditions. Ferroptosis caused by cysteine depletion plays a pivotal role sorafenib responses during hepatocellular carcinoma (HCC) therapy. However, it is not known whether macropinocytosis functions as alternative acquire sorafenib-treated HCC, and subsequently mitigates sorafenib-induced ferroptosis. This study aimed...
Abstract Recent studies have shown that the mammalian high-affinity copper transporter encoded by Ctr1 is involved in uptake of cisplatin. However, roles hCtr1 cisplatin-sensitive and cisplatin-resistant cells not been investigated. Here, we show that, five cell lines, only one (SR2) exhibited substantial reduction expression as compared with its sensitive line small lung cancers (SCLC), whereas efflux transporters ATP7A ATP7B were significantly altered. SR2 cross-resistance to carboplatin...
Obesity has been associated with reflux oesophagitis. However, the relationship between metabolic syndrome characterised by visceral obesity and oesophagitis is unclear.To investigate whether or a risk factor for oesophagitis.A cross-sectional study of 7078 subjects undergoing upper endoscopy during health check-ups was conducted (3539 patients vs age- sex-matched controls). We further analysed according to categories adipose tissue subcutaneous area 750 cases age-, sex- waist...
Although many reports have revealed the importance of defective microglia-mediated amyloid β phagocytosis in Alzheimer's disease (AD), underlying mechanism remains to be explored. Here we demonstrate that neurons brains patients with AD and mice show reduction sphingosine kinase1 (SphK1), leading microglial dysfunction inflammation resolution due decreased secretion specialized proresolving mediators (SPMs). Elevation SphK1 increased SPMs secretion, especially 15-R-Lipoxin A4, by promoting...
To develop and validate an in vivo cocktail method for high-throughput phenotyping of CYP1A2, CYP2C9, CYP2C19, CYP2D6, CYP3A, 12 healthy subjects received five probe drugs alone or simultaneously. The index the ratio 8 h urine concentration losartan to its metabolite after a single administration losartan, was not significantly different from that obtained using five-drug cocktail. Similarly, ratios [omeprazole]/[5-hydroxyomeprazole] (CYP2C19) [paraxanthine]/[caffeine] (CYP1A2) 4 plasma...
Objective This study was addressed to understand the underlying mechanism of substrate-dependent effect genetic variation in SLCO1B1, which encodes OATP1B1 (organic anion transporting polypeptide) transporter, on disposition two substrates, pravastatin and pitavastatin, relation their transport activities. Methods The uptake pravastatin, fluvastatin measured oocytes overexpressing SLCO1B1*1a SLCO1B1*15 compare alterations in-vitro activity. After 40-mg or 4-mg pitavastatin administered 11...
Genetic variants of three human organic cation transporter genes (<i>hOCT</i>s) were extensively explored in a Korean population. The functional changes <i>hOCT2</i> evaluated vitro, and those genetic polymorphisms <i>hOCT</i>s compared among different ethnic populations. From direct DNA sequencing, 7 13 coding nonsynonymous single-nucleotide (SNPs), including four from <i>hOCT1</i> (F160L, P283L, P341L, M408V) (T199I, T201M, A270S), whereas 6 synonymous SNPs. linkage disequilibrium analysis...
Copper is an essential metal nutrient, yet copper overload toxic. Here, we report that human transporter (hCtr) 1 plays important role in the maintenance of homeostasis by demonstrating expression hCtr1 mRNA was up-regulated under copper-depleted conditions and down-regulated copper-replete conditions. Overexpression full-length transfection with a recombinant <i>hCtr1</i> cDNA clone reduced endogenous levels, whereas overexpression N terminus-deleted did not change suggesting increased...
Genetic variants of Na+-taurocholate co-transporting polypeptide (NTCP; SLC10A1) and ileal apical sodium-dependent bile acid transporter (ASBT; SLC10A2), which greatly contribute to homeostasis, were extensively explored in the Korean population functional NTCP compared among Asian populations.From direct DNA sequencing, six SNPs identified SLC10A1 gene 14 SLC10A2 gene. Three seven coding non-synonymous SNPs: two from (A64T, S267F) one (A171S). No linkage was analysed because low frequencies...
To overcome the low oral bioavailability of morin, a mixed micelle formulation with pharmaceutical excipients that facilitate solubilization and modulate P-glycoprotein (P-gp) was developed evaluated in vitro vivo rats. Morin-loaded morin-PluronicF127-Tween80 ratio 1 : 10 0.02 (w/w/w) prepared by thin-film hydration method. The solubility, size distribution, drug encapsulation efficiency, percent loading were characterized. Subsequently, pharmacokinetic parameters morin loaded PluronicF127...
We aimed to develop a sensitive method for detecting 13 ginsenosides using liquid chromatography-tandem mass spectrometry and apply this pharmacokinetic studies in human following repeated oral administration of red ginseng extract. The chromatograms Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3, Rh2, F1, compound K (CK), protopanaxadiol (PPD), protopanaxatriol (PPT) plasma were well separated. calibration curve range was 0.5-200 ng/mL the lower limit quantitation 0.5 all ginsenosides. inter- intra-day...
We aimed to develop a berberine formulation enhance the intestinal absorption and plasma concentrations of through inhibition P-glycoprotein (P-gp)-mediated efflux metabolism in rats. used pluronic P85 (P85) tween 80, which have potential inhibit P-gp cytochrome P450s (i.e., CYP1A2, 2C9, 2C19, 2D6, 3A4). A berberine-loaded mixed micelle with ratios berberine: P85: 80 1:5:0.5 (w/w/w) was developed. This had mean size 12 nm increased cellular accumulation digoxin via inhibition. It also...
The purpose of this study was to investigate the effect genetic variations in organic anion-transporting polypeptide 1B1 (OATP1B1) and Na+/taurocholate co-transporting (NTCP) on uptake various statins having different affinities for these transporters.The functional activities simultaneous expression NTCP OATP1B1 were confirmed by taurocholate estrone-3-sulphate as representative substrates OATP1B1, respectively, an immunofluorescence analysis. substrate specificities effects rosuvastatin,...
Previous studies have demonstrated that treating cultured cells with cisplatin (CDDP) up-regulated the expression of glutathione (GSH) and its de novo rate-limiting enzyme glutamate-cysteine ligase (GCL), which consists a catalytic (GCLC) modifier (GCLM) subunit. It has also been shown many CDDP-resistant cell lines exhibit high levels GCLC/GCLM GSH. Because GSH system is major intracellular regulator redox conditions serve as an important detoxification cytoprotector, these results taken...
Fucoidan is an l-fucose-enriched sulfated polysaccharide isolated from brown algae and marine invertebrates. In this study, we investigated the protective effect of fucoidan Fucus vesiculosus on alcohol-induced murine liver damage. Liver injury was induced by oral administration 25% alcohol with or without (30 mg/kg 60 mg/kg) for seven days. Alcohol increased serum aspartate aminotransferase alanine levels, but these increases were suppressed treatment fucoidan. Transforming growth factor...
The aim of this study was to prepare a solid dispersion formulation curcumin enhance its solubility, dissolution rate, and oral bioavailability. prepared with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) mannitol using solvent evaporation freeze-drying methods, which yielded composed curcumin, TPGS, at ratio 1:10:15 (w/w/w). solubility rate the markedly improved compared those powder physical mixture mannitol. About 90% released from within 10 min. After administering orally...
As a central feature of neuroinflammation, microglial dysfunction has been increasingly considered causative factor neurodegeneration implicating an intertwined pathology with amyloidogenic proteins. Herein, we report the smallest synthetic molecule ( N , ′-diacetyl- p -phenylenediamine [DAPPD]), simply composed benzene ring 2 acetamide groups at para position, known to date as chemical reagent that is able promote phagocytic aptitude microglia and subsequently ameliorate cognitive defects....
Abstract Sphingosine kinase1 (SphK1) is an acetyl-CoA dependent acetyltransferase which acts on cyclooxygenase2 (COX2) in neurons a model of Alzheimer’s disease (AD). However, the mechanism underlying this activity was unexplored. Here we show that N-acetyl sphingosine (N-AS) first generated by and through SphK1. N-AS then acetylates serine 565 (S565) COX2, N-AS-acetylated COX2 induces production specialized pro-resolving mediators (SPMs). In mouse AD, microglia reduction generation, leading...
Genetic polymorphisms of the organic cation transporter 2 (OCT2), encoded by SLC22A2, have been investigated in association with metformin disposition. A functional decrease transport function has shown to be associated OCT2 variants. Using metabolomics, our study aims at a comprehensive monitoring primary metabolite changes order understand biochemical alteration and discovery potential endogenous metabolites related genetic variation OCT2. GC-TOF MS based profiling, clear clustering...