Abstract The development of wearable energy sto rage and harvesting devices is pivotal for advancing next-generation healthcare technologies, facilitating continuous real-time health monitoring. Traditional have been constricted by bulky rigid batteries, limiting their practicality comfort. However, recent advancements in materials science enabled the creation flexible, stretchable, lightweight storage solutions. integration technologies essential developing self-sustaining systems that...

This study aims to analyze the development trend of municipal solid waste (MSW) treatment in China from 2011 2020, and provide insights into challenges opportunities sustainable MSW management. Our analysis shows that generation declined which could be attributed a combination factors, including low urban population growth rate, implementation garbage classification policy, impact COVID-19 pandemic. We also found shift landfill incineration is expected increase significantly coming years,...

Copper is an essential metal nutrient, yet copper overload toxic. Here, we report that human transporter (hCtr) 1 plays important role in the maintenance of homeostasis by demonstrating expression hCtr1 mRNA was up-regulated under copper-depleted conditions and down-regulated copper-replete conditions. Overexpression full-length transfection with a recombinant <i>hCtr1</i> cDNA clone reduced endogenous levels, whereas overexpression N terminus-deleted did not change suggesting increased...

Copper is an essential micronutrient for cell growth but toxic in excess. transporter (Ctr1) plays important role regulating adequate copper levels mammalian cells. We have shown previously that expression of the human high-affinity (hCtr1) was transcriptionally up-regulated under copper-depleted conditions and down-regulated replete conditions; moreover, elevated hCtr1 suppress expression. Specificity protein 1 (Sp1) regulates copper-stressed conditions. In this study, we made following...

Platinum-based antitumor agents are widely used in cancer chemotherapy. Drug resistance is a major obstacle to the successful use of these because once drug develops, other effective treatment options limited. Recently, we conducted clinical trial using copper-lowering agent overcome platinum ovarian patients and preliminary results encouraging. In supporting this study, three pairs cisplatin (cDDP)-resistant cell lines two derived from who had failed platinum-based chemotherapy, showed that...

Arachidonic acid (AA) is the major precursor of several classes signal molecules and alteration its metabolism involved in human carcinogenesis. For instance, 5-lipoxygenase (5-LOX) converts AA to hydroxyeicosatetraenoic acids or leukotrienes (LTs), which are able enhance proliferation, increase survival suppress apoptosis cells. To determine potential use 5-LOX inhibitors prevention esophageal cancer, we first analyzed expression tissue samples using immunohistochemistry then examined...

Previous studies have demonstrated that treating cultured cells with cisplatin (CDDP) up-regulated the expression of glutathione (GSH) and its de novo rate-limiting enzyme glutamate-cysteine ligase (GCL), which consists a catalytic (GCLC) modifier (GCLM) subunit. It has also been shown many CDDP-resistant cell lines exhibit high levels GCLC/GCLM GSH. Because GSH system is major intracellular regulator redox conditions serve as an important detoxification cytoprotector, these results taken...

Neutrophil NETosis is a unique form of cell death, characterized by the release decondensed chromatin and antimicrobial contents to extracellular space, which involved in inflammation thrombosis. However, role pathogenesis immune-mediated thrombotic thrombocytopenic purpura (iTTP) how targeted therapy affects accumulation neutrophil traps (NETs) under flow remain unknown. Flow cytometry demonstrated that percentage neutrophils undergoing whole blood from patients with iTTP on admission was...

BACKGROUND: ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 repeats, 13), primarily synthesized in hepatic stellate endothelial cells, plays a pivotal role the regulation of hemostasis by proteolytic cleavage von Willebrand factor. Severe deficiency plasma activity may result thrombotic thrombocytopenic purpura, potentially fatal blood disorder. undergoes posttranslational modifications including glycosylation, citrullination, oxidation. The present study determines...

Mutations in 5’-untranslated region (5’-UTR) of ANKRD26 (ankyrin repeat domain-containing protein 26) are associated with hereditary thrombocytopenia-2 (THC2). However, the causative role these mutations and mechanisms underlying THC2 not fully established. Here, we report for first time that zebrafish carrying a deletion two nucleotides (Δ2) 5’-UTR ankrd26 recapitulate some key laboratory features THC2. Ankrd26ku6 (homozygous Δ2 5’-UTR) fish larvae exhibited significantly increased...

The human high-affinity copper transporter (hCtr1) plays an important role in the regulation of intracellular homeostasis. hCtr1 is involved transport platinum-based antitumor agents such as cisplatin (CDDP); however, mechanisms that regulate hCtr1-mediated these have not been well elucidated. We compared and CDDP. found replacements several methionine residues are essential for conferred a dominant-negative effect on endogenous hCtr1's function, resulting reduced rates Cu(I) CDDP increased...

Thrombotic thrombocytopenic purpura (TTP), a potentially fatal blood clot disorder, is primarily caused by severe deficiency of plasma ADAMTS13 activity resulting from acquired autoantibodies. Plasma exchange the only effective initial therapy. However, high mortality rate and complications associated with therapy remain major concern. To address unmet clinical needs, therapeutic efficacies anfibatide, snake venom-derived platelet glycoprotein Ib antagonist, in murine models spontaneous...

Objective— ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 repeats-13) cleaves VWF (von Willebrand factor). This process is essential for hemostasis. Severe deficiency of plasma activity, most commonly resulting from autoantibodies against ADAMTS13, causes thrombotic thrombocytopenic purpura. Therapeutic exchange the standard care to date, which removes replenishes ADAMTS13. However, such a therapy often ineffective raise in-hospital mortality rate remains as high 20%....

Thrombotic thrombocytopenic purpura (TTP) is caused by severe deficiency of ADAMTS13 (A13), a plasma metalloprotease that cleaves endothelium-derived von Willebrand factor (VWF). However, A13 alone often not sufficient to cause an acute TTP; additional factors may be required trigger the disease. Using CRISPR/Cas9, we created and characterized several novel zebrafish lines carrying null mutation in a13-/- , vwf, both. We further used these test hypothesis inflammation results neutrophil...

Abstract The expression of retinoic acid receptor β2 (RAR-β2) is frequently lost in various cancers and their premalignant lesions. However, the restoration RAR-β2 inhibits tumor cell growth suppresses cancer development. To understand molecular mechanisms responsible for this RAR-β2-mediated antitumor activity, we did restriction fragment differential display-PCR cloned a novel retinoid receptor–induced gene 1 (RRIG1), which differentially expressed RAR-β2-positive RAR-β2-negative cells....

Objective: Interactions between the renin-angiotensin system (RAS) and transforming growth factor-beta 1 (TGF-β1) have been well documented. The aim was to explore effect of irbesartan combining with emodin on myocardial remodeling in goldblatt (2K-1C) hypertensive rats. Methods: All 60 Sprague-Dawley rats were randomly divided into five groups: sham-clipped without any drugs; 2K-1C Irbesartan (50mg/kg/day); Emodin (80mg/Kg/day); together for last eight weeks a 12-week period study. outcome...

Abstract Esophageal cancer is a substantial health problem because of its usually late stage at diagnosis and poor prognosis. Tobacco smoking alcohol use are the most important risk factors in development esophageal squamous cell carcinoma (SCC). Our previous study demonstrated binding benzo[a]pyrene diol epoxide (BPDE), carcinogen present tobacco smoke environmental pollution, to ataxia‐telangiectasia mutated (ATM) gene. To understand how this affects alteration ATM expression identify...

Abstract We previously showed that induction of retinoid receptor–induced gene-1 (RRIG1) expression inhibited RhoA activation and tumor cell colony formation, invasion, proliferation, these effects are associated with the suppression extracellular signal-regulated protein kinases 1 2 phosphorylation cyclooxygenase-2 expression. To further elucidate its role in growth, gene expression, tumorigenesis, we determined RRIG1 breast esophageal tissue specimens then stably transfected into a TE-8...

Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is a potentially fatal blood disorder, resulting from autoantibodies against ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13). However, the mechanism underlying anti-ADAMTS13 autoantibody formation not known, nor it known how genetic aberrations contribute to pathogenesis of iTTP. Here we performed whole exome sequencing (WES) DNA samples 40 adult patients iTTP 15 local healthy subjects no...

Rationale: Moxibustion, an important therapeutic measure of TCM, can stimulate acupoints to unblock the meridians and collaterals, regulate function qi blood, support health, expel pathogens. So it could be effective safe for treatment constipation improvement quality life in poststroke patients with constipation. Patient concerns: He has a history constipation, defecation hard, bound stool every 2 3 days help glycerin enema. Diagnoses: Constipation >6 months; Cerebral infarction 9 Type...

Prenatal ethanol exposure can cause a broad range of abnormalities in newborns known as Fetal Alcohol Spectrum Disorder (FASD). Despite significant progress understanding the disease mechanisms FASD, there remains strong global need for effective therapies. To evaluate therapeutic potential sulforaphane (SFN), an active compound extracted from cruciferous vegetables, preventing ethanol-exposed zebrafish embryos were pretreated, co-treated, or post-treated with various concentrations SFN. The...