A5089565189- Cancer Genomics and Diagnostics
- Oral microbiology and periodontitis research
- Dental Implant Techniques and Outcomes
- Periodontal Regeneration and Treatments
- Dental Radiography and Imaging
- Genetic factors in colorectal cancer
- Oral Health Pathology and Treatment
- Magnesium Alloys: Properties and Applications
- RNA Research and Splicing
- Aluminum Alloys Composites Properties
- Breast Cancer Treatment Studies
- BRCA gene mutations in cancer
- Salivary Gland Disorders and Functions
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Dental Health and Care Utilization
- Dental materials and restorations
- Oral and gingival health research
- Endodontics and Root Canal Treatments
- RNA and protein synthesis mechanisms
- HIV/AIDS oral health manifestations
- Immune Response and Inflammation
- Streptococcal Infections and Treatments
- Lung Cancer Treatments and Mutations
- Oral and Maxillofacial Pathology
Pusan National University
2016-2025
Pusan National University Dental Hospital
2016-2025
Chung-Ang University
2025
Kolon Life Science (South Korea)
2023
Life Science Institute
2022
Kyungpook National University
2015-2020
Memorial Sloan Kettering Cancer Center
2018-2020
University of Florida
2013-2014
Yonsei University
2006-2012
Rutgers New Jersey Medical School
2005-2012
The 3' untranslated regions (3' UTRs) of mRNAs contain cis-acting elements for posttranscriptional regulation gene expression. Here, we report that mouse genes tend to express with longer UTRs as embryonic development progresses. This global is controlled by alternative polyadenylation and coordinates initiation organogenesis aspects development, including morphogenesis, differentiation, proliferation. Using myogenesis C2C12 myoblast cells a model, recapitulated this process in vitro found...
Abstract Background Alternative polyadenylation is one of the mechanisms in human cells that give rise to a variety transcripts from single gene. More than half genes have multiple sites (poly(A) sites), leading variable mRNA and protein products. Previous studies individual indicated alternative could occur tissue-specific manner. Results We set out systematically investigate occurrence mechanism different tissues using bioinformatic approaches. Using expressed sequence tag (EST) data, we...
mRNA polyadenylation and pre-mRNA splicing are two essential steps for the maturation of most human mRNAs. Studies have shown that some genes generate variants involving both alternative splicing. Polyadenylation in introns can lead to conversion an internal exon a 3′ terminal exon, which is termed composite or usage otherwise skipped, skipped exon. Using cDNA/EST genome sequences, we identified sites all currently known genes. We found ∼20% at least one intronic event potentially variants,...
Atherosclerotic vascular disease is a leading cause of myocardial infarction and cerebrovascular accident, independent associations with periodontal (PD) are reported. PD caused by polymicrobial infections aggressive immune responses. Genomic DNA Porphyromonas gingivalis, the best-studied bacterial pathogen associated severe PD, detected within atherosclerotic plaque. We examined causal relationships between chronic P. gingivalis oral infection, atherosclerosis in hyperlipidemic ApoEnull...
Upon mating, females of many animal species undergo dramatic changes in their behavior. In Drosophila melanogaster , postmating behaviors are triggered by sex peptide (SP), which is produced the male seminal fluid and transferred to female during copulation. SP modulates via receptor (SPR) located a small subset internal sensory neurons that innervate uterus project CNS. Although required for responses only these neurons, SPR expressed broadly CNS both sexes. Moreover, also encoded genomes...
Polyadenylation of nascent transcripts is one the key mRNA processing events in eukaryotic cells. A large number human and mouse genes have alternative polyadenylation sites, or poly(A) leading to variants with different protein products and/or 3'-untranslated regions (3'-UTRs). PolyA_DB 2 contains sites identified for several vertebrate species, including human, mouse, rat, chicken zebrafish, using alignments between cDNA/ESTs genome sequences. Several new features been added database since...
Background Dramatic changes in gene expression occur response to extracellular stimuli and during differentiation. Although transcriptional effects are important, alterations mRNA decay also play a major role achieving rapid massive abundance. Moreover, just as transcription factor activity varies between different cell types, the factors influencing cell-type specific. Principal Findings We have established rates of for over 7000 transcripts expressed mouse C2C12 myoblasts. found that...
Although many reports have revealed the importance of defective microglia-mediated amyloid β phagocytosis in Alzheimer's disease (AD), underlying mechanism remains to be explored. Here we demonstrate that neurons brains patients with AD and mice show reduction sphingosine kinase1 (SphK1), leading microglial dysfunction inflammation resolution due decreased secretion specialized proresolving mediators (SPMs). Elevation SphK1 increased SPMs secretion, especially 15-R-Lipoxin A4, by promoting...
mRNA polyadenylation is an essential step for the maturation of almost all eukaryotic mRNAs, and tightly coupled with termination transcription in defining 3′-end genes. Large numbers human mouse genes harbor alternative sites [poly(A) sites] that lead to variants containing different 3′-untranslated regions (UTRs) and/or encoding distinct protein sequences. Here, we examined conservation divergence types poly(A) across human, mouse, rat chicken. We found 3′-most tend be more conserved than...
Abstract: Although a range of biological and pharmacological activities melatonin have been reported, little is known about its potential anti‐inflammatory efficacy in periodontal disease. In this study, we investigated the effects on production inflammatory mediators by murine macrophages stimulated with lipopolysaccharide (LPS) from Prevotella intermedia , major cause reactions periodontium, sought to determine underlying mechanisms action. Melatonin suppressed nitric oxide (NO)...
Objective To investigate the effect of extracorporeal shock wave therapy (ESWT) on myofascial pain syndrome (MPS). Method Thirty patients with MPS in trapezius muscle were randomly divided into two groups, ESWT group (n=15), and trigger point injections (TPI)+transcutaneous electrical nerve stimulation (TENS) (n=15). For a total 3 weeks, was undertaken 1,500 pulse each time at one week interval totaling 4,500 pulse, TPI for once three times TENS five weeks. Results The changes threshold...
Periodontal disease (PD) and atherosclerosis are both polymicrobial multifactorial although observational studies supported the association, causative relationship between these two diseases is not yet established. Polymicrobial infection-induced periodontal postulated to accelerate atherosclerotic plaque growth by enhancing risk factors of orally infected Apolipoprotein E deficient (ApoEnull) mice. At 16 weeks infection, samples blood, mandible, maxilla, aorta, heart, spleen, liver were...
Mucinous carcinoma of the breast (MCB) is a rare histologic form estrogen receptor (ER)-positive/HER2-negative cancer (BC) characterized by tumor cells floating in lakes mucin. We assessed genomic landscape 32 MCBs whole-exome sequencing and/or RNA-sequencing. GATA3 (23.8%), KMT2C (19.0%), and MAP3K1 (14.3%) were most frequently mutated genes pure MCBs. In addition, two recurrent but not pathognomonic fusion genes, OAZ1-CSNK1G2 RFC4-LPP, detected 3/31 (9.7%) 2/31 (6.5%) samples,...
Treponema denticola is a predominantly subgingival oral spirochete closely associated with periodontal disease and has been detected in atherosclerosis. This study was designed to evaluate causative links between induced by chronic T. infection atherosclerosis hyperlipidemic ApoE(-/-) mice. mice (n = 24) were orally infected ATCC 35404 euthanized after 12 24 weeks. genomic DNA plaque samples, indicating colonization of the cavity. Infection elicited significantly (P 0.0172) higher IgG...
Abstract Although the repertoire of somatic genetic alterations primary breast cancers has been extensively catalogued, differences between and metastatic tumors have less studied. In this study, we compared mutations gene copy number their matched metastases from patients with estrogen receptor (ER)–negative disease. DNA samples obtained formalin-fixed paraffin-embedded ER-negative/HER2-positive (n = 9) ER-, progesterone (PR-), HER2-negative 8) paired brain or skin normal tissue were...
Sphingolipids are ubiquitous building blocks of eukaryotic cell membranes that function as signaling molecules for regulating a diverse range cellular processes, including proliferation, growth, survival, immune-cell trafficking, vascular and epithelial integrity, inflammation. Recently, several studies have highlighted the pivotal role sphingolipids in neuroinflammatory regulation. multiple functions, induction expression various inflammatory mediators regulation neuroinflammation by...
Ductal carcinoma in situ (DCIS) is a nonobligate precursor of invasive breast cancer. Here, we sought to investigate the level intralesion genetic heterogeneity DCIS and patterns clonal architecture changes progression from disease.Synchronous (n = 27) ductal carcinomas no special type (IDC-NSTs; n 26) 25 patients, pure 7) 7 patients were microdissected separately subjected high-depth whole-exome 56) or massively parallel sequencing targeting ≥410 key cancer-related genes 4). Somatic...
Periodontitis is caused by dysbiosis of oral microbes and associated with increased cognitive decline in Alzheimer's disease (AD), recently, a potential functional link was proposed between AD. We compared the microbiomes patients or without AD to evaluate association periodontitis.
Lobular carcinoma in situ (LCIS) is a preinvasive lesion of the breast. We sought to define its genomic landscape, whether intralesion genetic heterogeneity present LCIS, and clonal relatedness between LCIS invasive breast cancers.Experimental Design: reanalyzed whole-exome sequencing (WES) data performed targeted amplicon validation mutations identified 43 27 synchronous more clinically advanced lesions from 24 patients [9 ductal carcinomas (DCIS), 13 lobular (ILC), 5 (IDC)]. Somatic...
Mono-allelic germline pathogenic variants in the Partner And Localizer of BRCA2 (PALB2) gene predispose to a high-risk breast cancer development, consistent with role PALB2 homologous recombination (HR) DNA repair. Here, we sought define repertoire somatic genetic alterations PALB2-associated cancers (BCs), and whether BCs display bi-allelic inactivation and/or genomic features HR-deficiency (HRD). Twenty-four patients mutations were analyzed by whole-exome sequencing (WES, n = 16) or...
Periodontitis is a chronic and multifactorial inflammatory disease that can lead to tooth loss. At present, the diagnosis for periodontitis primarily based on clinical examination radiographic parameters. Detecting periodontal pathogens at subgingival plaque requires skilled professionals collect samples. Periodontal are also detected various mucous membranes in patients with periodontitis. In this study, we characterized oral microbiome profiles from buccal mucosa supragingival space total...
Abstract Sphingosine kinase1 (SphK1) is an acetyl-CoA dependent acetyltransferase which acts on cyclooxygenase2 (COX2) in neurons a model of Alzheimer’s disease (AD). However, the mechanism underlying this activity was unexplored. Here we show that N-acetyl sphingosine (N-AS) first generated by and through SphK1. N-AS then acetylates serine 565 (S565) COX2, N-AS-acetylated COX2 induces production specialized pro-resolving mediators (SPMs). In mouse AD, microglia reduction generation, leading...
Periodontitis is a widespread chronic inflammatory disease caused by interactions between periodontal bacteria and homeostasis in the host. We aimed to investigate performance reliability of machine learning models predicting severity periodontitis. Mouthwash samples from 692 subjects (144 healthy controls 548 generalized periodontitis patients) were collected, genomic DNA was isolated, copy numbers nine pathogens measured using multiplex qPCR. The are as follows: Porphyromonas gingivalis...