A5030904312- Oral microbiology and periodontitis research
- Oral Health Pathology and Treatment
- Streptococcal Infections and Treatments
- Salivary Gland Disorders and Functions
- Alzheimer's disease research and treatments
- Infective Endocarditis Diagnosis and Management
- S100 Proteins and Annexins
- Immune Response and Inflammation
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Aortic aneurysm repair treatments
- Protease and Inhibitor Mechanisms
- Head and Neck Cancer Studies
- Aortic Disease and Treatment Approaches
- Connexins and lens biology
- Proteoglycans and glycosaminoglycans research
- Bone and Dental Protein Studies
- Natural Antidiabetic Agents Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Oral health in cancer treatment
- Dental materials and restorations
- Bone Metabolism and Diseases
- SARS-CoV-2 detection and testing
- Infectious Aortic and Vascular Conditions
- Hippo pathway signaling and YAP/TAZ
Texas A&M University
2022-2024
University of Florida
2013-2021
Florida College
2018-2020
Periodontal disease is a polymicrobial inflammatory that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute the development Alzheimer's disease. ApoE-/- mice were orally infected (n = 12) with Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium nucleatum as mono- infections. sacrificed following 12 24 weeks infection. Bacterial genomic DNA was isolated from all brain tissues except for F....
Atherosclerotic vascular disease is a leading cause of myocardial infarction and cerebrovascular accident, independent associations with periodontal (PD) are reported. PD caused by polymicrobial infections aggressive immune responses. Genomic DNA Porphyromonas gingivalis, the best-studied bacterial pathogen associated severe PD, detected within atherosclerotic plaque. We examined causal relationships between chronic P. gingivalis oral infection, atherosclerosis in hyperlipidemic ApoEnull...
Background: Evidence suggest periodontal bacterial infection can contribute to oral cancer initiation and progression. Aim: To investigate the effects of bacteria on cell behavior using a cell-based system mouse carcinogenesis model. Methods: Oral lines were polyinfected with four bacteria. Cytokine levels relative changes in oncogene mRNA expression determined post-infection. tumours mice induced by 4-nitroquinoline-1-oxide (4NQO) compared without administrating Results: Polyinfected cells...
Periodontal disease (PD) and atherosclerosis are both polymicrobial multifactorial although observational studies supported the association, causative relationship between these two diseases is not yet established. Polymicrobial infection-induced periodontal postulated to accelerate atherosclerotic plaque growth by enhancing risk factors of orally infected Apolipoprotein E deficient (ApoEnull) mice. At 16 weeks infection, samples blood, mandible, maxilla, aorta, heart, spleen, liver were...
Periodontal disease (PD) develops from a synergy of complex subgingival oral microbiome, and is linked to systemic inflammatory atherosclerotic vascular (ASVD). To investigate how polybacterial microbiome infection influences plaque progression, we infected the cavity ApoEnull mice with consortium 4 well-characterized periodontal pathogens, Porphyromonas gingivalis, Treponema denticola, Tannerealla forsythia Fusobacterium nucleatum, that have been identified in human by DNA screening. We...
Tannerella forsythia is a Gram-negative anaerobic organism that inhabits the subgingival cavity and initiates connective tissue destruction alveolar bone resorption in periodontal disease (PD). PD chronic immunoinflammatory has been linked to several systemic diseases including atherosclerosis. This study evaluated effects of oral infection with T. ATCC 43037 on induction PD, inflammatory markers atherosclerosis risk factors hyperlipidemic ApoE(null) mice. Mice were orally infected for 12 24...
Treponema denticola is a predominantly subgingival oral spirochete closely associated with periodontal disease and has been detected in atherosclerosis. This study was designed to evaluate causative links between induced by chronic T. infection atherosclerosis hyperlipidemic ApoE(-/-) mice. mice (n = 24) were orally infected ATCC 35404 euthanized after 12 24 weeks. genomic DNA plaque samples, indicating colonization of the cavity. Infection elicited significantly (P 0.0172) higher IgG...
ABSTRACT The American Heart Association supports an association between periodontal diseases and atherosclerosis but not a causal association. This study explores the use of integrin β6 −/− mouse model to causality. We investigated ability polymicrobial consortium Porphyromonas gingivalis , Treponema denticola Tannerella forsythia Fusobacterium nucleatum colonize periodontium induce local systemic inflammatory responses. Polymicrobially infected Itgβ6 mice demonstrate greater susceptibility...
This study explored the origin of age-related granules in apolipoprotein E gene knockout (ApoE-/-) B6 background mice brains following chronic gingival infection with Porphyromonas gingivalis for 24 weeks. Intracerebral localization P. was detected by fluorescence situ hybridization (FISH) and its protease immunohistochemistry. The were observed periodic acid-Schiff (PAS), silver impregnation, immunostaining. FISH showed intracerebral dissemination cells (p = 0.001). PAS impregnation...
It has been previously hypothesized that oral microbes may be an etiological link between rheumatoid arthritis (RA) and periodontal disease. However, the mechanistic basis of this association is incompletely understood. Here, we investigated role bacteria in induction joint inflammation collagen-induced (CIA) B10.RIII mice. CIA-prone mice were infected orally with a polybacterial mixture Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia for 24 weeks before CIA. The ability...
Background: Periodontitis is a chronic, polymicrobial inflammatory disease that degrades connective tissue and alveolar bone results in tooth loss. Oxidative stress has been linked to the onset of periodontal breakdown systemic inflammation, success antiresorptive treatments will rely on how effectively they can ameliorate disease–induced oxidative during oral infection. Methods: Rats were infected with polybacterial inoculum consisting Porphyromonas gingivalis , Treponema denticola...
The American Heart Association supports an association between periodontal disease (PD) and atherosclerotic vascular (ASVD) but does not as of yet support a causal relationship. Recently, we have shown that major pathogens Porphyromonas gingivalis Treponema denticola are causally associated with acceleration aortic atherosclerosis in ApoEnull hyperlipidemic mice. aim this study was to determine if oral infection another significant pathogen Fusobacterium nucleatum can accelerate inflammation...
Toll-like-receptors (TLRs) play a significant role in the generation of specific innate immune response against invading pathogens. TLR2 and TLR4 signaling contributes to infection-induced inflammation periodontal disease (PD) atherosclerosis. Observational studies point towards relationship between PD atherosclerosis, but recognition multiple oral pathogens their modulation host leading atherosclerosis are not clear. We evaluated induction both TLR2-/- TLR4-/- mice polymicrobial infection...
Peri-implantitis is a complex polymicrobial biofilm-induced inflammatory osteolytic gingival infection that results in orofacial implant failures. To the best knowledge of authors, there are no preclinical vivo studies dentistry have investigated response to known microbial biofilms observed humans. The aim this study develop novel peri-implant rat model using an established periodontitis.Wistar rats were used for experimental peri-implantitis. One month after extraction maxillary first...
Altered microRNA expression is implicated in cardiovascular diseases. Our objective was to determine signatures thoracic aortic aneurysms (TAAs) and abdominal (AAAs) compared with control non-aneurysmal specimens. We evaluated the of fifteen selected human TAA AAA operative specimens controls. observed significant upregulation miR-221 downregulation miR-1 -133 In contrast, miR-146a miR-145 -331-3p were found only for Upregulation miR-126 -486-5p miR-30c-2*, -155, -204 TAAs AAAs. The data...
The polymicrobial dysbiotic subgingival biofilm microbes associated with periodontal disease appear to contribute developing pathologies in distal body sites, including the brain. This study examined oxidative stress, form of increased protein carbonylation and damage, tumor necrosis factor-α (TNF-α) transgenic mouse that models inflammatory TNF-α excess during bacterial infection; apolipoprotein knockout (ApoE-/-) brains, following Porphyromonas gingivalis gingival monoinfection. Following...
Periodontal disease (PD) and atherosclerotic vascular (ASVD) are both chronic inflammatory diseases with a polymicrobial etiology have been epidemiologically associated. The purpose is to examine whether periodontal bacteria that infect the periodontium can also tissues enhance pre-existing early aortic lesions in LDLRnull mice. Mice were orally infected intermediate bacterial colonizer Fusobacterium nucleatum for first 12 weeks followed by late colonizers (Porphyromonas gingivalis,...
Background: Cleavage of the amyloid-β protein precursor (AβPP) mediated by host secretase enzymes, releases several fragments including (Aβ 40 and Aβ 42 ). Objective: To determine if Porphyromonas gingivalis conditioned medium cleaved AβPP to release . Methods: The SH-SY5Y cell line was challenged, in vitro, with P. (Pg381) presence/absence cytokines. cells their supernatants were assessed for cleavage immunoblotting transmission electron microscopy. Results: Western blotting lysates...
Plasma membrane-associated Toll-like receptor (TLR2 and TLR4) signaling contributes to oral microbe infection-induced periodontitis atherosclerosis. We recently reported that either TLR2 or TLR4 deficiency alters recognition of a consortium pathogens, modifying host responses in evaluated the effects combined TLR2−/−TLR4−/− double knockout mice on innate immune induction atherosclerosis after polybacterial infection with Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia...
Periodontal disease is a highly prevalent chronic inflammatory and associated with complex microbial infection in the subgingival cavity. Recently, American Heart Association supported century old association between periodontal atherosclerotic vascular disease. We have recently shown that polybacterial led to aortic atherosclerosis modulation of lipid profiles; however underlying mechanism(s) has not been yet demonstrated. Altered nitric oxide (NO) synthesis tetrahydrobiopterin (BH4),...
Extracellular matrix (ECM) stiffness influences cancer cell fate by altering gene expression. Previous studies suggest that stiffness-induced nuclear deformation may regulate expression through YAP localization. We investigated the role of lamina in this process. show exhibits mechanical threshold behavior: once unwrinkled, is inextensible. A computational model predicts unwrinkled under tension, which confirmed using a lamin tension sensor. Laminar unwrinkling caused flattening during...