Periodontitis is a widespread chronic inflammatory disease caused by interactions between periodontal bacteria and homeostasis in the host. We aimed to investigate performance reliability of machine learning models predicting severity periodontitis. Mouthwash samples from 692 subjects (144 healthy controls 548 generalized periodontitis patients) were collected, genomic DNA was isolated, copy numbers nine pathogens measured using multiplex qPCR. The are as follows: Porphyromonas gingivalis...

Abstract Background Type 2 diabetes mellitus (DM) is a complex metabolic disorder that causes various complications, including periodontitis (PD). Although bidirectional relationship has been reported between DM and PD, their immunological remains poorly understood. Therefore, this study aimed to compare the immune response in patients with PD alone those both (PDDM) expand our knowledge of complicated connection DM. Methods Peripheral blood mononuclear cells were collected from 11 healthy...

Background/Objectives: As non-thermal atmospheric pressure plasma (NTP) is known to have advantages in application the medical field, we consider its applicability periodontitis, a representative chronic inflammatory disease. The purpose of this study was evaluate effect NTP inhibiting progression periodontitis rat model when additionally used scaling and root planing (SRP). Methods: To induce experimental 20 rats, ligatures were placed maxillary second molar lipopolysaccharide from...

Background/Objectives: This study aims to investigate the relationship between serum fatty acid-binding protein 4 (FABP4) levels and severity of periodontitis in systemically healthy individuals. Additionally, examines whether non-surgical periodontal treatment can reduce FABP4 levels, establishing its potential as a biomarker linking systemic diseases. Methods: A total 89 participants with stage I, II, or III were recruited, excluding individuals Clinical parameters such clinical attachment...

This study examined the prevalence and risk factors of peri-implant disease after at least 7 years dental implant loading.A total 111 patients with 218 implants were treated. The follow-up period for all was years. patients' records collected investigated through logistic regression analysis.The overall survival rate 95.87%, because 9 failed. mucositis peri-implantitis 39.7% 16.7%, respectively. As factors, smoking prosthetic splinting showed significant associations (P<0.05).Within limits...

The objective of this study was to analyze the microbial profile individuals with peri-implantitis (PI) compared those periodontally healthy (PH) subjects and periodontitis (PT) using Illumina sequencing.Buccal, supragingival, subgingival plaque samples were collected from 109 (PH: 30, PT: 49, PI: 30). V3-V4 region 16S rRNA sequenced analyzed microbiota.Microbial community diversity in PI group higher than other groups, 3 groups showed significantly separated clusters buccal samples....

This study was undertaken to evaluate the clinical and microbiological effects of an erythritol powder air-polishing device (EPAP) as a supplement scaling root planing (SRP) therapy in patients with moderate chronic periodontitis.Clinical evaluations were performed at 21 sites treated SRP (control) SRP+EPAP (test). All examinations before treatment, 1 month after 3 months treatment.There no significant differences between test group control group. Microbiological analysis revealed that...

The aim of this study was to evaluate the ability Porphyromonas gingivalis (P. gingivalis) induce oxidation high-density lipoprotein (HDL) and determine whether oxidized HDL induced by P. exhibited altered antiatherogenic function or became proatherogenic.P. THP-1 monocytes were cultured, extent evaluated a thiobarbituric acid-reactive substances (TBARS) assay. To proatherogenic properties gingivalis-treated HDL, lipid quantified TBARS assay, tumor necrosis factor alpha (TNF-α) levels...

Abstract Type 2 diabetes mellitus (T2DM) and periodontitis (PD) have intricated connections as chronic inflammatory diseases. While the immune response is a key factor that accounts for their association, underlying mechanisms remain unclear. To gain deeper understanding of connection, we conducted research using multiomics approach. We generated whole genome methylation profiling array data from periodontium PD patients with DM (PDDM) without to confirm genetic epigenetic changes....

Purpose: Few studies have examined periodontal pathogens from saliva samples in periodontally healthy young adults.The purposes of this study were to determine the prevalence periodontopathic bacteria and quantify using real-time polymerase chain reaction (PCR) assays Korean adults under 35 years age.Methods: Nine major analyzed by PCR 94 adults.Quantification Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia,...

Cathepsin K (CTSK) is a cysteine protease that mainly produced from mature osteoclasts and contributes to the destruction of connective tissues mineralized matrix as consequence periodontal disease (PD). However, few studies have reported its regulatory role in osteoclastogenesis-supporting cells inflammatory conditions. Here, we investigated CTSK cells, focusing on modulation paracrine function. Microarray data showed was upregulated PD patients compared with healthy individuals, which...

This study aimed to profile differentially expressed (DE) exosomal RNAs in healthy subjects and periodontitis patients compare their levels before after treatment.Plasma samples from with (pre-/post-periodontal treatment) were collected for this case-control study. After isolation of exosomes the plasma, RNA was extracted small sequencing performed (3 samples, 4 pre-treatment 5 post-treatment samples). Two-way analyses conducted according treatment status group, unpaired analysis (grouping...

A strong correlation between chronic periodontitis and systemic diseases (e.g., cardiovascular disease, metabolic disorders) has been suggested for several decades. However, the evidence supporting this is restricted primarily to epidemiologic studies, with only a few experimental outcomes confirming such providing information about underlying molecular mechanisms. To reveal as well relevant pathway, we investigated effects of Porphyromonas gingivalis Fusobacterium nucleatum, which play...

Abstract Background Periodontitis is a major inflammatory disease of the oral mucosa that not limited to cavity but also has systemic consequences. Although importance chronic periodontitis been emphasized, immune response induced by and its therapeutic effects remain elusive. Here, we report transcriptomes peripheral blood mononuclear cells (PBMCs) from patients with periodontitis. Methods Using single-cell RNA sequencing, profiled PBMCs healthy controls paired pre- post-treatment We...

Periodontitis is an infectious disease that associated with microorganisms colonize the tooth surface. Clinically, periodontal condition stability reflects dynamic equilibrium between bacterial challenge and host response. Therefore, pathogen assessment can assist in early detection of periodontitis. Here we developed a grading system called index (PPI) by analyzing copy numbers multiple pathogens both healthy chronic periodontitis patients. We collected 170 mouthwash samples (64...

This study aimed to apply fluoride formulations enamel with cold atmospheric plasma (CAP) and analyze the uptake, retention, acid resistance quantitatively. Human specimens were divided randomly into 2 groups: group APF1, 1.23% acidulated phosphate (APF) gel; APF2, APF gel CAP. Fluoride CAP applied samples 4 times at 1-wk intervals. The also stored in artificial saliva for wk evaluate retention of fluoride. content on fluoride-treated was measured by an electron probe microanalyzer. To...

Although periodontal pathogens show a strong association with development of atherosclerosis, little is known about how microorganism contributes to disease onset and progression. Oxidation low-density lipoprotein (LDL) major risk factor atherogenesis. The principal objective this study evaluate the ability peptide 19 (Pep19) Porphyromonas gingivalis (Pg) heat shock protein (HSP) as potent inducer LDL oxidation, secondary compare that Pep19 from different bacteria.HSP60, Pep14, Pg THP-1...

Foam cells are one of the major cellular components atherosclerotic plaques, within which trace periodontal pathogens has also been identified in recent studies. In line with these findings, correlation between periodontitis and cardiovascular incidences repetitively supported by evidence from a number experimental However, direct role altered signaling underlying such events not clearly defined. To determine pathogenesis atherosclerosis, especially evolution macrophages into foam cells, we...

Purpose:The purpose of the present study was to perform a pattern analysis in patients with temporomandibular disorder (TMD) resulting from unilateral mastication due chronic periodontitis.Methods: Thirty participants signs or symptoms TMD who engaged periodontitis-related discomfort (test group) were selected.Another 30 subjects exhibiting not periodontitis (control also recruited.An interview-based questionnaire administered, and an examination joint (TMJ) determination periodontal status...

The aim of this study was to investigate the relationship between serum uric acid (SUA) levels and risk periodontitis in Korean adults using data from National Health Nutrition Examination Survey (KNHANES).This cross-sectional used KNHANES 2016-2018 analysed 12,735 aged ≥19 years who underwent oral examinations. Hypouricemia defined as SUA <3 mg/dL men <2 women, hyperuricemia ≥7 ≥6 women.The weighted prevalence hypouricemia 0.6% 12.9%, respectively. overall rate 30.5%. frequency subjects...

The present study investigated the outcomes of a newly-developed, simple, and practical nonsurgical treatment modality suitable for most forms intrabony defects around failing dental implants using intrasulcular delivery chlorhexidine solution minocycline hydrochloride (HCl).Forty-five in 20 patients diagnosed with peri-implantitis were included. At baseline endpoint, probing pocket depth (PPD), clinical attachment level (CAL), presence bleeding on (BOP) at 6 sites each implant recorded....

The keys to successful esthetic implant therapy in the anterior region are natural-looking restorations and an adequate alveolar ridge volume. Destruction of periodontal tissue resulting from and/or endodontic inflammation causes problems. Forced eruption is a valuable adjunct augmenting soft hard where insufficient for esthetics. In this article, forced was adopted site development prior placement augmented vertically using technique. This technique simplified surgical procedure without...

Periodontitis is an inflammatory disease caused by Porphyromonas gingivalis (P. gingivalis) in the oral cavity. This periodontal causes damage to ligament and alveolar bone can cause tooth loss, but there no definite treatment yet. In this study, we investigated possibility of using no-ozone cold plasma safely treat periodontitis First, human gingival fibroblasts (HGFs) were treated with P. gingivalis-derived lipopolysaccharide (PG-LPS) induce response, then anti-inflammatory effect NCP was...

Previous studies have reported on changes in masticatory ability and patient satisfaction after restoration of missing second molars. However, there has been no evaluation the oral health-related quality life (OHRQoL) patients who did not replace their molar. The purpose present study was to analyze effects loss a molar OHRQoL. Twenty-two subjects had lost (the group) another 22 with complete dentition control were enrolled. In ability, an objective test by measurement mixing index (MAI)...