A5074769527- Breast Cancer Treatment Studies
- Cancer Genomics and Diagnostics
- Breast Lesions and Carcinomas
- Thyroid Cancer Diagnosis and Treatment
- Gene expression and cancer classification
- Metabolomics and Mass Spectrometry Studies
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Cancer Diagnosis and Treatment
- Mass Spectrometry Techniques and Applications
- HER2/EGFR in Cancer Research
- Molecular Biology Techniques and Applications
- Bone Tumor Diagnosis and Treatments
- BRCA gene mutations in cancer
- Cancer and Skin Lesions
- Salivary Gland Tumors Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Cancer Immunotherapy and Biomarkers
- Advanced Breast Cancer Therapies
- Global Cancer Incidence and Screening
- Oral and Maxillofacial Pathology
- Microtubule and mitosis dynamics
- Advanced Proteomics Techniques and Applications
- Lymphoma Diagnosis and Treatment
- RNA modifications and cancer
Universidade Federal do Rio Grande do Sul
2025
San Antonio College
2024
Instituto de Saúde
2024
Fundação de Amparo à Pesquisa do Estado do Amazonas
2024
AC Camargo Hospital
2014-2023
Hospital São Paulo
2019-2020
Memorial Sloan Kettering Cancer Center
2010-2019
Hospital São Lucas da PUCRS
2016
Pontifícia Universidade Católica do Rio Grande do Sul
2016
Kettering University
2010-2014
Abstract There is a clinical need to predict sensitivity of metastatic hormone receptor-positive and HER2-negative (HR+/HER2−) breast cancer endocrine therapy, targeted RNA sequencing (RNAseq) offers diagnostic potential measure both transcriptional activity functional mutation. We developed the SET ER/PR index gene expression microarray probe sets that were correlated with receptors ( ESR1 PGR ) robust preanalytical analytical influences. tested in biopsies metastastic HR+/HER2− against...
Purpose The increased breast cancer risk conferred by a diagnosis of lobular carcinoma in situ (LCIS) is poorly understood. Here, we review our 29-year longitudinal experience with LCIS to evaluate factors associated risk. Patients and Methods participating surveillance after an are observed prospectively maintained database. Comparisons were made among women choosing surveillance, or without chemoprevention, those undergoing bilateral prophylactic mastectomies between 1980 2009. Results One...
Clinically meaningful molecular subtypes for classification of breast cancers have been established, however, initiation and progression these remain poorly understood. The recent development desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) facilitates the convergence analytical chemistry traditional pathology, allowing chemical profiling with minimal tissue pretreatment in frozen samples. Here, we characterized composition cancer DESI-MSI. Regions interest were...
BACKGROUND Infections with multiple human papillomavirus (HPV) types (mHPV) in Papanicolaou tests have been reported but the histologic correlation and clinical meaning remains debatable. METHODS The authors prospectively tested 37 HPV using Linear Array Genotyping Test correlated results to cytology histology findings 260 women evaluated from June 2009 October 2011 followed for up 60 months. RESULTS was detected 148 of 235 samples (63%) high‐risk 132 (56%). mHPV infection found be twice as...
Circulating tumor cells are important markers of progression and can reflect behavior in metastatic colorectal cancer (mCRC). Identification proteins that confer resistance to treatment is an step predict response better selection for patients. Multidrug resistance-associated protein 1 (MRP1) 4 (MRP4) play a role irinotecan-resistance, Excision Repair Cross-Complementation group (ERCC1) expression platinum compounds. Here, we included 34 patients with mCRC most them received FOLFIRI or...
Lobular carcinoma in situ (LCIS) is a preinvasive lesion of the breast. We sought to define its genomic landscape, whether intralesion genetic heterogeneity present LCIS, and clonal relatedness between LCIS invasive breast cancers.Experimental Design: reanalyzed whole-exome sequencing (WES) data performed targeted amplicon validation mutations identified 43 27 synchronous more clinically advanced lesions from 24 patients [9 ductal carcinomas (DCIS), 13 lobular (ILC), 5 (IDC)]. Somatic...
O projeto de extensão “UFRGS para Todos”, desenvolvido pelo Grupo PET Engenharia Civil, atua em escolas públicas ensino médio, apresentando a UFRGS e formas ingresso nas universidades (ENEM vestibular) por meio atividades integração dinâmicas lúdicas. Realizado encontros presenciais, o visa (a) aproximar estudantes da universidade, (b) fornecer informações muitas vezes inacessíveis, (c) responder dúvidas sobre (d) incentivar acesso às Universidades Federais.
While acute tissue injury potently induces endogenous danger signal expression, the role of these molecules in chronic wound healing and lymphedema is undefined. The purpose this study was to determine spatial temporal expression patterns signals high-mobility group box 1 (HMGB1) heat shock protein (HSP)70 during lymphatic fluid stasis. In a surgical mouse tail model lymphedema, HMGB1 HSP70 occurred along gradient relative site injury, with peak at greater than twofold reduced within 5 mm (P...
The PI3K/PTEN pathway plays a major role in carcinogenesis. Dysregulation of this occurs frequently breast cancer, and loss PTEN expression is emerging as potentially important mechanism resistance to the widely used anti-HER2 therapy, trastuzumab. However, assays for have suffered from lack consistency. Here, we describe an automated reliable protocol protein by immunohistochemistry formalin-fixed paraffin-embedded tissue that can be easily incorporated into clinical trials.
Purpose: Lobular carcinoma in situ (LCIS) has been proposed as a non‐obligate precursor of invasive lobular (ILC). Here we sought to define the repertoire somatic genetic alterations pure LCIS and synchronous ILC using targeted massively parallel sequencing. Methods: DNA samples extracted from microdissected LCIS, matched normal breast tissue or peripheral blood 30 patients were subjected sequencing targeting all exons 273 genes, including genes most frequently mutated cancer repair‐related...
Abstract Purpose: To assess the prevalence of phosphoinositide 3-kinase (PI3K) pathway alterations in pure high-grade ductal carcinoma situ (DCIS) and DCIS associated with invasive breast cancer (IBC), to determine whether adjacent IBCs harbor distinct PI3K aberrations. Experimental Design: Eighty-nine cases 119 IBC were characterized according estrogen receptor (ER) HER2 status, subjected immunohistochemical analysis PTEN, INPP4B, phosphorylated (p)AKT pS6 expression, microdissection...
Triple-negative mammary carcinoma (TNBC) is an aggressive breast cancer subtype associated with dismal prognosis. The interaction between the immune system and cells plays a crucial role in tumor development progression. However, it still unclear how each diverse cell of contributes to prognosis patients cancer. In this study, we investigated composition infiltrated modifies survival resected TNBC.Retrospectively, collected data from 76 diagnosed non-metastatic TNBC available tissue blocks...
Abstract Purpose: Fine-needle aspiration (FNA) cytology, a standard method for thyroid nodule diagnosis, cannot distinguish between benign follicular adenoma (FTA) and malignant carcinoma (FTC). Previously, using expression profiling, we found that combination of transcript levels from DDIT3, ARG2, C1orf24, ITM1 distinguished FTA FTC. The goal this study was to determine if antibody markers used alone or in could accurately wider variety lesions fixed sections FNA samples. Experimental...
Abstract Introduction Lobular carcinoma in situ (LCIS) has been accepted as a marker of risk for the development invasive breast cancer, yet modern models carcinogenesis include LCIS precursor low-grade carcinomas. We provide evidence favoring clonal origin and synchronous estrogen receptor-positive malignant lesions ductal lobular phenotype. Methods Patients with prior undergoing mastectomy were identified preoperatively from 2003 to 2008. Specimens widely sampled, frozen blocks screened...
Recent evidence suggests that lobular carcinoma in situ (LCIS) can be a clonal precursor of invasive breast cancers both the ductal and phenotypes. We sought to confirm these findings with an extensive study fresh frozen specimens from women undergoing mastectomy. Patients history LCIS presenting for therapeutic mastectomy were identified prospectively. Frozen tissue blocks collected, screened lesions interest, subjected microdissection DNA extraction. Copy number profiling, whole-exome...
Breast cancer stromal compartment, may influence responsiveness to chemotherapy. Our aim was detect a cell signature (using direct approach of microdissected cells) associated with response neoadjuvant chemotherapy (neoCT) in locally advanced breast (LABC). The tumor samples were collected from 44 patients LABC (29 estrogen receptor (ER) positive and 15 ER negative) before the start any treatment. Neoadjuvant consisted doxorubicin cyclophosphamide, followed by paclitaxel. Response defined as...
Annexin A1 (AnxA1) is a pleiotropic protein that exerts essential roles in breast cancer (BC) growth and aggressiveness. In our previous work, we described the autocrine signaling of AnxA1 through formyl peptide receptor 1 (FPR1) triple-negative (TN) BC cell line, MDA-MB-231. Here, aimed to describe interaction between AnxA1/FPR1 Interleukin-6 (IL-6) pathways their role tumor microenvironment (TME). First, demonstrated IL-6 expression levels are correlated tissue samples. three TNBC lines,...