A5024791362- Cancer Cells and Metastasis
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Sarcoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Cancer Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Gastric Cancer Management and Outcomes
- Brain Metastases and Treatment
- Colorectal Cancer Surgical Treatments
- Metastasis and carcinoma case studies
- Lung Cancer Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Inflammatory Biomarkers in Disease Prognosis
- COVID-19 and healthcare impacts
- Lung Cancer Diagnosis and Treatment
- Cancer Research and Treatments
- Cancer, Lipids, and Metabolism
- Head and Neck Cancer Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Immunotherapy and Biomarkers
- Cancer, Stress, Anesthesia, and Immune Response
- Colorectal and Anal Carcinomas
- Glioma Diagnosis and Treatment
AC Camargo Hospital
2014-2023
Institut de Virologie
2021
Royal Brisbane and Women's Hospital
2021
University of Liverpool
2021
Society of Surgical Oncology
2021
Hospital Italiano de Buenos Aires
2021
Sarcomas are rare and heterogeneous neoplasms with poor prognosis that thought to spread distant organs mainly by hematogenous dissemination. However, circulating tumor cells (CTCs) have never been visualized in sarcomas.To investigate the feasibility of using isolation size (ISET) for isolation, identification, characterization CTCs derived from patients high-grade metastatic sarcomas.We studied eleven metastatic/recurrent or locally advanced soft-tissue sarcomas (STSs), six whom had...
Circulating tumor cells are important markers of progression and can reflect behavior in metastatic colorectal cancer (mCRC). Identification proteins that confer resistance to treatment is an step predict response better selection for patients. Multidrug resistance-associated protein 1 (MRP1) 4 (MRP4) play a role irinotecan-resistance, Excision Repair Cross-Complementation group (ERCC1) expression platinum compounds. Here, we included 34 patients with mCRC most them received FOLFIRI or...
Thymidylate synthase (TYMS) is an important enzyme for 5-fluorouracil (5-FU) metabolism in metastatic colorectal cancer (mCRC) patients. The search this circulating tumor cells (CTCs) can be a powerful tool to follow-up mCRC patients were enrolled before the beginning of 5-FU-based chemotherapy. blood was filtered on Isolation by Size Epithelial Tumor Cells (ISET), and analysis TYMS expression CTCs made immunocytochemistry. Additionally, we verified staining primary tumors metastases from...
Background: Quantification of Circulating Tumor Cells (CTCs) as a prognostic marker in metastatic colorectal cancer (mCRC) has already been validated and approved for routine use. However, more than quantification, qualification or characterization CTCs is gaining importance, since the genetic may reflect, real time fashion, profile disease. Objective: To characterize KRAS mutations (codon 12 13) from patients with mCRC to compare matched primary tumor. Additionally, correlate these clinical...
Neoadjuvant chemoradiation (NCRT) followed by total mesorectal excision is the standard treatment for locally advanced rectal cancer (LARC). To justify a non-surgical approach, identification of pathologic complete response (pCR) required. Analysis circulating tumor cells (CTCs) can be used to evaluate pCR. We hypothesize that monitoring thymidylate synthase (TYMS) and repair protein, RAD23 homolog B (RAD23B), predict resistance chemotherapy/radiotherapy. Therefore, aims this study were...
Circulating tumor cells (CTCs) have been reported to be a relevant prognostic biomarker in metastatic patients. However, their clinical use and impact is still under debate. We thus comparatively kinetically assessed two CTC detection methods according the patient's follow up.CTC counting characterization were repeatedly performed during up patient with undifferentiated non-small cell lung cancer by using cytokeratin (CK)-dependent immunomagnetic separation (Miltenyi) CK-independent,...
Abstract Background Gastric adenocarcinoma (GAC) is the third deadliest malignant neoplasm worldwide, mostly because of late disease diagnosis, low chemotherapy response rates, and an overall lack tumor biology understanding. Therefore, tools for prognosis prediction treatment are needed. Quantification circulating cells (CTCs) microemboli (CTM) their expression biomarkers has potential clinical relevance. Our aim was to evaluate CTCs CTM HER2 plakoglobin in patients with nonmetastatic GAC,...
Colorectal cancer (CRC) is the third most prevalent worldwide. New prognostic markers are needed to identify patients with poorer prognosis, and circulating tumor cells (CTCs) seem be promising accomplish this.A prospective study was conducted by blood collection from metastatic CRC (mCRC), three times, every 2 months in conjunction image examinations for evaluation of therapeutic response. CTC isolation counting were performed Isolation Size Epithelial Tumor Cells (ISET).A total 54 mCRC a...
Abstract Background Circulating tumor microemboli (CTM) are clusters of circulating cells (CTCs), involved in metastasis, as also transforming growth factor‐β (TGF‐β). The purpose this study was to verify their role progression‐free survival (PFS). Methods Blood from patients with locally advanced head and neck squamous cell carcinoma (HNSCC; n = 53) analyzed 2 moments. TGF‐β receptor I (TGF‐βRI) expression evaluated by immunocytochemistry. Results Comparing CTM1 (baseline) CTM2 (first...
Soft tissue Sarcomas (STS) are rare malignances, with high mortality rates. Half of patients develop metastasis. The presence isolated Circulating Tumor Cells (CTCs) and Microemboli (CTM) in the blood may be early markers tumor invasion. Epidermal Growth Factor (EGF) family receptors can also influence this process.to quantify CTCs identify CTM as well EGF Receptor (EGFR) protein expression these cells correlate clinical outcome metastatic STS.Approximately 8mL was prospectively collected...
Cancer is primarily a disease in which late diagnosis linked to poor prognosis, and unfortunately, detection management are still challenging. Circulating tumor cells (CTCs) potential resource address this disease. Cell fusion, an event discovered recently CTCs expressing carcinoma leukocyte markers, occurs when ≥2 become single entity (hybrid cell) after the merging of their plasma membranes. fusion poorly understood despite continuous evaluations vitro/in vivo studies. Blood samples from...
Colorectal cancer is a common and often deadly cancer. Circulating tumor cells (CTCs) have been implicated as potentially valuable prognosis factor. The detection of circulating microemboli (CTM) simple blood component parameters that reflect inflammatory status, such the platelet-to-lymphocyte ratio (PLR) neutrophil-to-lymphocyte (NLR), may provide information about progression. aim this study was to explore importance CTCs, CTM, PLR, NLR prospectively in non-metastatic colon CTCs were...
The heterogeneity of response to neoadjuvant chemoradiotherapy (NCRT) is still a challenge in locally advanced rectal cancer (LARC). evaluation thymidylate synthase (TYMS) and RAD23 homolog B (RAD23B) expression circulating tumor cells (CTCs) provides complementary clinical information. CTCs were prospectively evaluated 166 blood samples (63 patients) with LARC undergoing NCRT. primary objective was verify if the absence RAD23B/TYMS would correlate pathological complete (pCR). Secondary...
The discovery of predictive biomarkers in metastatic colorectal cancer (mCRC) is essential to improve clinical outcomes. Recent data suggest a potential role circulating tumor cells (CTCs) as prognostic indicators. We conducted follow-on analysis from prospective study consecutive patients with mCRC. CTC was at two timepoints: baseline (CTC1; before starting chemotherapy), and months after treatment (CTC2). isolation/quantification were completed by ISET® (Rarecells, France). expressions...
Metastasis is common in the diagnosis of pancreatic cancer, and presence epithelial-mesenchymal transition markers circulating tumor cells may suggest worse prognosis.To correlate number (CTCs) peripheral blood patients with a locally advanced or metastatic protein expression involved (EMT) CTCs clinical characteristics, progression-free survival (PFS) overall (OS).This was prospective study conducted using samples collected at three different times. were quantified by ISET test analyzed...
Desmoid tumor (DT) is a rare neoplasm with high local recurrence rates, composed of fibroblastic cells that are characterized by the expression key molecules, including intermediate filament vimentin, cyclooxygenase-2 (COX-2), and nuclear β-catenin, lack epithelial markers. Circulating (CTCs) isolated from peripheral blood patients sarcomas other neoplasms can be used as early biomarkers invasion dissemination. Moreover, CTCs also re-colonize their tumors origin through process "tumor...
Abstract Gastric cancer (GC) is the fifth most common type of worldwide with high incidences in Asia, Central, and South American countries. This patchy distribution means that GC studies are neglected by large research centers from developed The need for further understanding this complex disease, including local importance epidemiological factors rich ancestral admixture found Brazil, stimulated implementation GE4GAC project. aims to embrace epidemiological, clinical, molecular...
HER2 expression switching in circulating tumor cells (CTC) breast cancer is dynamic and may have prognostic predictive clinical implications. In this study, we evaluated the association between of CTC patients with brain metastases (BCBM) disease control. An exploratory analysis a prospective assessment before (CTC1) after (CTC2) stereotactic radiotherapy/radiosurgery (SRT) for BCBM 39 women was performed. Distant failure-free survival (DBFFS), primary endpoint, overall (OS) were estimated....
Circulating tumor cells (CTCs) play an important role in progression and metastasis, particularly form of cluster, which is called circulating microemboli (CTM), can be found the peripheral blood cancer patients. The aim this study was to evaluate presence CTCs CTM, its influence on lung were isolated by Isolation Size Epithelial Tumor (ISET®) Technology. samples metastatic patients collected before beginning systemic chemotherapy. CTM identified according their morphological features....
203 Background: Colorectal cancer (CRC) is one of the most common worldwide. Around 30% present metastatic disease at diagnosis and 50%–60% patients develop metastasis. New prognostic markers are needed circulating tumor cells (CTCs) a promising tool. Methods: Prospective study conducted by blood collection from 75 (pts) with CRC (mCRC), twice, 2 months interval, together image exams for therapeutic response evaluation. CTCs were detected ISET identified immunocytochemistry. Results: The...
Predicting the risk of early distant brain failure (DBF) is in demand for management decisions patients who are candidates local treatment metastases. This study aimed to analyze association between circulating tumor cells (CTCs) and disease control after stereotactic radiation therapy/radiosurgery (SRT) breast cancer metastasis (BCBM).We prospectively assessed CTCs before (CTC1) 4 5 weeks (CTC2) SRT their relationship with number new lesions (NL) suggestive BCBM SRT. CTC were quantified...
Ductal adenocarcinoma of the pancreas is fourth most common cancer-associated cause death in Western world. The presence circulating tumor cells (CTCs) can be considered a potential prognostic factor, as these represent progression, allowing monitoring therapeutic efficacy. objectives this study were to explore morphological, molecular, and phenotypic characteristics CTCs from blood patients with pancreatic carcinoma correlate findings response treatment, progression-free survival, overall...