A5074781158- Genital Health and Disease
- Cervical Cancer and HPV Research
- Thyroid Cancer Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Nutrition, Genetics, and Disease
- Urological Disorders and Treatments
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Urologic and reproductive health conditions
- BRCA gene mutations in cancer
- Cancer-related Molecular Pathways
- Prostate Cancer Treatment and Research
- Sarcoma Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
- Head and Neck Cancer Studies
- Sexual Differentiation and Disorders
- Esophageal Cancer Research and Treatment
- Forensic and Genetic Research
- Cancer Diagnosis and Treatment
- Ear and Head Tumors
- DNA Repair Mechanisms
Instituto do Câncer do Estado de São Paulo
2023-2024
Universidade de São Paulo
2010-2024
AC Camargo Hospital
2013-2022
Fundação de Amparo à Pesquisa do Estado de São Paulo
2014-2019
National Council for Scientific and Technological Development
2019
Hospital São Paulo
2010-2015
Universidade Estadual Paulista (Unesp)
2014-2015
Hospital de Câncer de Barretos
2014
Abstract Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor−node−metastasis prognosis classification. Therefore, defining biological signatures that allow assessing prognostic outcomes for OSCC patients would be great clinical significance. Using histopathology-guided discovery proteomics, we analyze neoplastic islands stroma from invasive tumor front (ITF) inner to identify differentially...
Circulating nucleic acids are found in free form body fluids and may serve as minimally invasive tools for cancer diagnosis prognosis. Only a few studies have investigated the potential application of circulating mRNAs microRNAs (miRNAs) prostate (PCa). The Cancer Genome Atlas (TCGA) database was used an silico analysis to identify mRNA miRNA markers PCa. A total 2,267 genes 49 miRNAs were differentially expressed between normal tumor samples. prediction analyses target integrative...
Papillary thyroid carcinoma (PTC) is a common endocrine neoplasm with recent increase in incidence many countries. Although PTC has been explored by gene expression and DNA methylation studies, the regulatory mechanisms of on was poorly clarified. In this study, profile (Illumina HumanMethylation 450K) 41 paired non-neoplastic adjacent tissues (NT) carried out to identify contribute elucidation role novel genic intergenic regions beyond those described promoter CpG islands (CGI). An...
The molecular mechanisms underlying penile carcinoma are still poorly understood, and the detection of genetic markers would be great benefit for these patients. In this study, we assessed genomic profile aiming at identifying potential prognostic biomarkers in carcinoma. Globally, 46 samples were considered to evaluate DNA copy-number alterations via array comparative hybridization (aCGH) combined with human papillomavirus (HPV) genotyping. Specific genes investigated by using qPCR, FISH,...
Even though the majority of well-differentiated thyroid carcinoma (WDTC) is indolent, a number cases display an aggressive behavior. Cumulative evidence suggests that deregulation DNA methylation has potential to point out molecular markers associated with worse prognosis. To identify prognostic epigenetic signature in cancer. Genome-wide assays (450k platform, Illumina) were performed cohort 50 nonneoplastic tissues (NTs), 17 benign lesions (BTLs), and 74 carcinomas (60 papillary, 8...
Despite penile carcinoma (PeCa) being a relatively rare neoplasm, it remains an important public health issue for poor and developing countries. Contrary to most tumors, limited data are available markers that capable of assisting in diagnosis, prognosis, treatment PeCa. We aimed identify molecular PeCa by evaluating their epigenomic transcriptome profiles comparing them with surrounding non-malignant tissue (SNT) normal glans (NG).
// Bruna Sugita 1 , Mandeep Gill 2 Akanskha Mahajan Anju Duttargi Saurabh Kirolikar Rodrigo Almeida Kenny Regis Olusayo L. Oluwasanmi Fabio Marchi 3 Catalin Marian 4, 8 Kepher Makambi 2, 5 Bhaskar Kallakury 6 Laura Sheahan 7 Iglenir J. Cavalli Enilze M. Ribeiro Subha Madhavan Simina Boca Yuriy Gusev Luciane R. Department of Genetics, Federal University Paraná, Curitiba, PR, Brazil Oncology, Lombardi Comprehensive Cancer Center, Georgetown Medical Washington, DC, USA International Research...
Thyroid nodules are common in adult population and papillary thyroid carcinoma (PTC) is the most frequent malignant finding. The natural history of PTC remains poorly understood current diagnostic methods limitations responsible for a significant number potentially avoidable surgeries. This study aimed to identify molecular markers improve diagnosis lesions. Gene expression profiling was performed using microarray 61 13 surrounding normal tissues (NT). A reliable gene list established...
Penile carcinoma (PeCa) is an important public health issue in poor and developing countries, has only recently been explored terms of genetic epigenetic studies. Integrative data analysis a powerful method for the identification molecular drivers involved cancer development progression. miRNA mRNA expression profiles followed by integrative were investigated 23 PeCa 12 non-neoplastic penile tissues (NPT). Expression levels eight miRNAs 10 mRNAs evaluated same set samples used microarray...
Molecular data generation and their combination in penile carcinomas (PeCa), a significant public health problem poor underdeveloped countries, remain virtually unexplored. An integrativemethodology combin ing genome-wide copy number alteration, DNA methylation, miRNA mRNA expression analysis was performed set of 20 usual PeCa. The well-ranked 16 driver candidates harboring genomic alterations regulated by miRNAs, including hsa-miR-31, hsa-miR-34a hsa-miR-130b, were significantly associated...
No AccessJournal of UrologyInvestigative Urology1 Jul 2015Down-Regulation SLC8A1 as a Putative Apoptosis Evasion Mechanism by Modulation Calcium Levels in Penile Carcinoma Juan J. Muñoz, Sandra A. Drigo, Mateus C. Barros-Filho, Fábio Marchi, Cristovam Scapulatempo-Neto, Gustavo S. Pessoa, Guimarães, José Carlos Trindade Filho, Ademar Lopes, Marco A.Z. Arruda, and Silvia R. Rogatto MuñozJuan Muñoz , DrigoSandra Drigo Barros-FilhoMateus Barros-Filho MarchiFábio Marchi...
Background Undifferentiated Pleomorphic Sarcoma (UPS) and high-grade Leiomyosarcoma (LMS) are soft tissue tumors with an aggressive clinical behavior, frequently developing local recurrence distant metastases. Despite several gene expression studies involving sarcomas, the potential to identify molecular markers has been limited, mostly due small sample size, in-group heterogeneity absence of detailed data. Materials Methods Gene profiling was performed for 22 LMS UPS obtained from untreated...
DNA methylation in miRNA genes has been reported as a mechanism that may cause dysregulation of mature miRNAs and consequently impact the gene expression. This is largely unstudied papillary thyroid carcinomas (PTC). To identify differentially methylated miRNA-encoding genes, we performed global analysis (Illumina 450 K), integrative (TCGA database), data confirmation (pyrosequencing RT-qPCR), functional assays. Methylation revealed 27 genes. The analyses pointed out miR-21 miR-146b...
The current treatment of laryngeal squamous cell carcinoma (LSCC) is based on radical surgery and radiotherapy resulting in high morbidity. Chemoradiotherapy has been used as alternative to organ sparing; however, several advanced cases presented resistance treatment, which contributes a risk recurrence mortality. Coding RNAs miRNAs have potential be biomarkers or targets for cancer therapy.In this study, 36 LSCC 5 non-neoplastic control samples were investigated using miRNA mRNA large-scale...
Cystic endometrial hyperplasia (CEH), mucometra, and pyometra are common uterine diseases in intact dogs, with being a life threatening disease. This study aimed to determine the gene expression profile of these lesions potential biomarkers for closed-cervix pyometra, most severe condition. Total RNA was extracted from 69 fresh endometrium samples collected 21 healthy female dogs during diestrus, 16 CEH, 15 mucometra 17 (eight open nine closed-cervixes). Global detected using Affymetrix...
Breast carcinoma (BC) corresponds to 23 % of all cancers in women, with 1.38 million new cases and 460,000 deaths worldwide annually. Despite the significant advances identification molecular markers different modalities treatment for primary BC, ability predict its metastatic behavior is still limited. The purpose this study was identify novel associated distinct clinical outcomes a Brazilian cohort BC patients. We generated global gene expression profiles using tumor samples from 24...
Objectives To integrate mRNA and miRNA expression profiles of mucoepidermoid carcinomas (MECs) normal salivary gland (NSGs) tissue samples identify potential drivers. Material Methods Gene arrays were performed in 35 MECs six NSGs. Results We found 46 differentially expressed (DE) miRNAs 3,162 DE mRNAs. Supervised hierarchical clustering analysis the transcripts revealed two clusters both profiles, which distinguished MEC from NSG samples. The integrative miRNA-mRNA a network comprising 696...
Undifferentiated high-grade pleomorphic sarcomas (UPSs) display aggressive clinical behavior and frequently develop local recurrence distant metastasis. Because these often share similar morphological patterns with other tumors, particularly leiomyosarcomas (LMSs), classification by exclusion is used. In this study, array-based comparative genomic hybridization (array CGH) was used to analyze 20 UPS 17 LMS samples from untreated patients. The presented a lower frequency of alterations...
// Sabrina Daniela da Silva 1, 2, 3 , Fabio Albuquerque Marchi 4, 5 Bin Xu 2 Krikor Bijian Faisal Alobaid 1 Alex Mlynarek Silvia Regina Rogatto 4 Michael Hier Luiz Paulo Kowalski Moulay A. Alaoui-Jamali Department of Otolaryngology Head and Neck Surgery, Sir Mortimer B. Davis-Jewish General Hospital, Canada Segal Cancer Centre Lady Davis Institute for Medical Research, Departments Medicine, Oncology, Pharmacology Therapeutics, Faculty McGill University, Surgery Otorhinolaryngology, AC...
Abstract TRAF2, a RING finger adaptor protein, plays an important function in tumor necrosis factor (TNF)- and TNF-like weak inducer of apoptosis (TWEAK)-dependent signaling, particular during inflammatory immune responses. We identified functional interaction TRAF2 with focal adhesion (FA) signaling involving the kinase (FAK) regulation cell susceptibility to anoikis. Comparison TRAF2-proficient (TRAF2+/+) versus TRAF2-deficient (TRAF2−/−), FAK-proficient (FAK+/+) FAK-deficient (FAK−/−)...