- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Agronomic Practices and Intercropping Systems
- Plant tissue culture and regeneration
- Plant responses to elevated CO2
- Plant and animal studies
- Legume Nitrogen Fixing Symbiosis
- Potato Plant Research
- Plant Diversity and Evolution
- Plant Pathogens and Fungal Diseases
- Banana Cultivation and Research
- Plant Pathogens and Resistance
- Coastal wetland ecosystem dynamics
- Cassava research and cyanide
Virginia Tech
2017-2024
Universidad de Los Andes
2018-2020
Universidad Nacional de Colombia
2018
Plant microbiota play essential roles in plant health and crop productivity. Comparisons of community composition have suggested seed, soil, the atmosphere as reservoirs phyllosphere microbiota. After finding that leaves tomato (Solanum lycopersicum) plants exposed to rain carried a higher microbial population size than not rain, we experimentally tested hypothesis is thus-far-neglected reservoir Therefore, were compared with either treated concentrated microbiota, filter-sterilized or...
Cuscuta campestris is an obligate parasitic plant that requires a host to complete its life cycle. Parasite-host connections occur via haustorium, unique organ acts as bridge for the uptake of water, nutrients, and macromolecules. Research on often complicated by influences, but comparable systems growing parasite in absence do not exist. We developed axenic method grow C. artificial system (AHS). evaluated effects nutrients phytohormones haustoria development growth. Haustorium morphology...
Poor survival on plants can limit the efficacy of Biological Control Agents (BCAs) in field. Yet bacteria survive atmosphere, despite their exposure to high solar radiation and extreme temperatures. If conditions atmosphere are similar to, or more than, environmental plant surface, then precipitation may serve as a reservoir robust BCAs. To test this hypothesis, two hundred fifty-four rain-borne isolates were screened for vitro inhibition Erwinia amylovora, causal agent fire blight, well...
Summary Cuscuta campestris , a stem parasitic plant, has served as valuable model plant for the exploration of plant–plant interactions and molecular trafficking. However, major barrier to C. research is that method generate stable transgenic plants not yet been developed. Here, we describe development transformation protocol using various reporter genes (GFP, GUS, or RUBY) morphogenic ( CcWUS2 CcGRF / GIF ), leading robust Agrobacterium ‐mediated transformation. The stably transformed...
Summary Cuscuta campestris , a stem parasitic plant, has served as valuable model plant for the exploration of plant-plant interactions and molecular trafficking. However, major barrier to C. research is that method generate stable transgenic plants not yet been developed. Here, we describe development transformation protocol using various reporter genes (GFP, GUS, or RUBY) morphogenic ( CcWUS2 CcGRF/GIF ), ultimately leading robust Agrobacterium -mediated transformation. The stably...
Abstract The International Potato Center (CIP) genebank conserves and facilitates access to highly diverse germplasm of potato, sweetpotato, Andean roots tubers as a global public good for food security. While it is generally understood that material from the CIP has played an important role in release many CIP-related varieties grown by smallholder farmers lower-income countries, contribution not been evaluated quantitative terms. By applying relative provenance based on pedigree data, we...
Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, most important disease in this crop. There a paucity knowledge about metabolism and its relevance pathogenic process, with exception elucidation Xanthan biosynthesis route. Here, we report reconstruction genome-scale model Xpm insights it provides into plant-pathogen interactions. The model, iXpm1556, displayed 1556 reactions, 1527 compounds, 890 genes. Metabolic maps central amino acid carbohydrate...
A Correction to this paper has been published: https://doi.org/10.1007/s12571-020-01136-6
ABSTRACT Plant microbiota play essential roles in plant health and crop productivity. Comparisons of community composition have suggested seeds, soil, the atmosphere as reservoirs phyllosphere microbiota. After finding that leaves tomato ( Solanum lycopersicum ) plants exposed to rain carried a higher microbial population size than not rain, we experimentally tested hypothesis is so far neglected reservoir Rain were thus compared with either treated concentrated microbiota, filter-sterilized...
First paragraph Dodders ( Cuscuta spp.) are obligate parasitic plants that obtain water and nutrients from the stems of host via specialized feeding structures called haustoria. Dodder haustoria facilitate bi-directional movement viruses, proteins, mRNAs between parasite 1 , but functional effects these movements not clear. Here we show C. campestris accumulate high levels many novel microRNAs (miRNAs) while parasitizing Arabidopsis thaliana hosts. Many miRNAs 22 nts long, a usually rare...
Abstract Cuscuta campestris is an obligate parasitic plant that requires a host to complete its lifecycle. Parasite-host connections occur via haustorium, unique organ acts as bridge for the uptake of water, nutrients and macromolecules. Research on often complicated by influences, but comparable systems growing parasite in absence do not exist. We developed axenic method grow C. Artificial Host System (AHS). evaluated effects phytohormones haustoria development growth. Haustorium morphology...