To address the growing concern over antibiotic-resistant microbial infections in aquatic animals, we tested several promising alternative agents that have emerged as new drug candidates. Specifically, tilapia piscidins are a group of peptides possess antimicrobial, wound-healing, and antitumor functions. In this study, focused on piscidin 3 (TP3) TP4, which derived from Oreochromis niloticus, investigated their inhibition acute bacterial by infecting hybrid (Oreochromis spp.) with Vibrio...

Antimicrobial peptide tilapia piscidin 4 (TP4) from Oreochromisniloticus exhibits potent bactericidal and anti-tumorigenic effects. In a variety of cancers, the mutation status p53 is decisive factor for therapeutic sensitivity. Therefore, we investigated impact on TP4-induced cytotoxicity in glioblastoma cell lines molecular mechanisms that govern cytotoxic Both U87MG (wild-type/WT p53) U251 (mutant were sensitive to cytotoxicity. The necrosis inhibitors Necrostatin-1 GSK'872 attenuated...

Objective: Gastric cancer with peritoneal metastasis is considered to be final stage gastric cancer. One current treatment approach for this condition combined cytoreductive surgery hyperthermic intraperitoneal chemotherapy (HIPEC). However, the therapeutic mechanisms of HIPEC remain largely undescribed. Method: In order assess cellular effects in vitro, we treated AGS human adenocarcinoma cells or without 5-fluorouracil (5-Fu) at 37 °C 43 (hyperthermic temperature) 1 h followed by...

Gardnerella vaginalis is associated with bacterial vaginosis (BV). The virulence factors produced by G. are known to stimulate vaginal mucosal immune response, which largely driven activated macrophages. While Tilapia piscidin 4 (TP4), an antimicrobial peptide isolated from Nile tilapia, display a broad range of antibacterial functions, it unclear whether TP4 can affect macrophage polarization in the context BV. In this study, we used culture supernatants differentiation THP-1 and RAW264.7...

Synovial sarcoma is a rare but aggressive soft-tissue associated with translocation t(X;18). Metastasis occurs in approximately 50% of all patients, and curative outcomes are difficult to achieve this group. Since the efficacies current therapeutic approaches for metastatic synovial remain limited, new agents urgently needed. Tilapia piscidin 4 (TP4), marine antimicrobial peptide, known exhibit multiple biological functions, including anti-bacterial, wound-healing, immunomodulatory,...

Non-small cell lung cancer (NSCLC) is one of the most common and deadly cancers worldwide. Among NSCLC patients, almost half have wild-type epidermal growth factor receptor (EGFR WT). The primary therapeutic option for these EGFR WT patients chemotherapy, while with mutations more diverse options, including tyrosine kinase inhibitors. Moreover, worse chemotherapy response than mutant patients. Thus, an urgent need exists novel strategies to improve in Hedgehog signaling known be highly...

The cationic antimicrobial peptide epinecidin-1 was identified from Epinephelus coioides and possesses multiple biological functions, including antibacterial, antifungal, anti-tumor, immunomodulatory effects. In addition, suppresses lipopolysaccharide (LPS)-induced inflammation by neutralizing LPS ameliorating LPS/Toll-like receptor (TLR)-4 internalization. However, it is unclear whether the actions of depend on regulation TLR adaptor protein MyD88 or endogenous signaling antagonists, which...

Tilapia piscidin (TP) 4 is an antimicrobial peptide derived from Nile tilapia (Oreochromis niloticus), which shows broad-spectrum antibacterial activity and excellent cancer-killing ability in vitro vivo. Like many other peptides, TP4 treatment causes mitochondrial toxicity cancer cells. However, the molecular mechanisms underlying targeting of mitochondria remain unclear. In this study, we used a pull-down assay on A549 cell lysates combined with LC-MS/MS to discover that targets adenine...

Synovial sarcoma is a rare but highly malignant and metastatic disease. Despite its relative sensitivity to chemotherapies, the high recurrence low 5-year survival rate for this disease suggest that new effective therapeutic agents are urgently needed. Marine antimicrobial peptide epinecidin-1 (epi-1), which was identified from orange-spotted grouper (Epinephelus coioides), exhibits multiple biological effects, including bactericidal, immunomodulatory, anticancer activities. However,...

Non-small cell lung cancer (NSCLC) is among the deadliest cancers worldwide. Despite recent introduction of several new therapeutic approaches for disease, improvements in overall survival and progression-free have been minimal. Conventional treatments NSCLC include surgery, chemotherapy radiotherapy. Except these can impair a patient's immune system, leaving them susceptible to bacterial infections. As such, Staphylococcus aureus infections are commonly seen patients receiving chemotherapy,...

Aims Osteosarcoma is the most common primary bone malignancy among children and adolescents. We investigated whether benzamil, an amiloride analogue sodium-calcium exchange blocker, may exhibit therapeutic potential for osteosarcoma in vitro. Methods MG63 U2OS cells were treated with benzamil 24 hours. Cell viability was evaluated MTS/PMS assay, colony formation flow cytometry (forward/side scatter). Chromosome condensation, terminal deoxynucleotidyl transferase dUTP nick end labelling...

Marine antimicrobial peptide tilapia piscidin (TP) 4 possesses antibacterial and immune regulatory activities, making it a potentially valuable feed supplement in aquaculture. In this study, we expressed the TP4 peptide, originally identified from Oreochromis niloticus, yeast (Pichia pastoris) under control of an alcohol oxidase (Aox) 1 promoter. Recombinant (r)TP4 expression was induced by methanol. Transformation confirmed PCR, colony hybridization. For scalable production, transformed...

Comprehensive treatment comprising neoadjuvant laparoscopic HIPEC (L-HIPEC) and bidirectional intraperitoneal systemic induction chemotherapy (BISIC) followed by cytoreductive surgery (CRS) for gastric cancer with peritoneal carcinomatosis (PC) has been developed. However, its benefits patient selection criteria have not thoroughly investigated. We retrospectively reviewed 113 patients, 25 having received comprehensive (L-HIPEC, BISIC, then CRS-HIPEC; the BISIC group) 88 direct CRS-HIPEC...

Dysregulated hepatic steatosis may lead to chronic liver inflammation and nonalcoholic steatohepatitis (NASH). Recent studies have suggested that exendin-4, a glucagon-like peptide-1 agonist, be promising therapeutic for NASH. However, the molecular mechanisms underlying antihepatic actions of exendin-4 are not fully clear. Here, we demonstrate autophagy is activated by either palmitic acid (PA) or oleic (OA) in HepG2 cells, further enhances autophagy-lysosomal pathway. We show inhibition...

Methicillin resistant Staphylococcus aureus (MRSA) may be found on the skin, nose, and throats of long-term hospitalized patients. While MRSA infections are usually minor, serious death occur in immunocompromised or diabetic patients, after exposure to blood. This report demonstrates that antimicrobial peptide (AMP) epinecidin-1 (Epi-1) efficiently protects against infection a pyemia pig model. We first Epi-1 exhibits bactericidal activity MRSA. Next, pharmacokinetic analysis revealed was...

Esophageal squamous cell carcinoma (ESCC) is a common and fatal malignancy with an increasing incidence worldwide. Over the past decade, concurrent chemoradiotherapy (CCRT) or without surgery emerging therapeutic approach for locally advanced ESCC. Unfortunately, many patients exhibit poor response develop acquired resistance to CCRT. Once occurs, overall survival rate drops down rapidly proper further treatment options, poses critical clinical challenge ESCC therapy. Here, we utilized...