All stakeholders in cultural heritage share an interest fabrication methods and material technology. Until now for analysis of organic materials, particularly proteins, have not been widely available to researchers at institutions. This paper will describe analytical method the identification collagen-based materials from soft tissue sources show examples its application diverse museum objects. The method, peptide mass fingerprinting (PMF), uses enzymatic digestion extracted proteins produce...

A new methodology, affinity capillary electrophoresis−mass spectrometry (ACE-MS), is introduced as a solution-based approach for screening combinatorial libraries drug leads. The method allows on-line, one-step selection and structural identification of candidate ligands. ACE-MS demonstrated using the binding vancomycin to all-d-tri- tetrapeptides model system. Peptide different forms Fmoc-DDXX Fmoc-EXX containing up 361 compounds were successfully employed determine interacting motifs....

Multilayer poly(p-phenylene biphenyltetracarboximide) (BPDA-PDA) polyimide films of 172 μm total thickness (11.4 per layer) were prepared from the poly(amic acid) precursor solution through repetition a spin-coat/softbake/cure process. Wide-angle x-ray diffraction results indicate that molecules in multilayer are highly ordered along chain axes as well lateral direction and furthermore oriented film plane observed single-layer 11.4 thickness. The showed same dynamic mechanical properties...

A rugged, stable liquid sheath interfacing procedure for on-line CE/MS is described. This combines optimized component sizes, tapered capillary tips, and adjustment of the with respect to tube during electrospray operation (active positioning) establish a quickly reliably. The interface especially effective at low flow rates, rate 250 nL/min has been achieved. Active positioning also allows isoelectric focusing mass spectrometry in-probe focusing. In this application, retracted into tube,...

In medical diagnostics, detection of cells exhibiting specific phenotypes constitutes a paramount challenge. Detection technology must ensure efficient isolation (often rare) targets while eliminating nontarget background cells. Technologies exist for such investigations, but many require high levels expertise, expense, and multistep protocols. Increasing automation, miniaturization, availability technologies is an aim microfluidic lab-on-a-chip strategies. To this end, we present...

Abstract We present a substantially improved design and functionality of centrifugo‐magnetophoretic platform which integrates direct immunoseparation cost‐efficient, bright‐field detection cancer cells in whole blood. All liquid handling takes place disposable cartridge with geometry akin to conventional compact disc (CD). The instrumentation required process such “lab‐on‐a‐disc” can be as simple cost‐efficient the rotor on common optical drive. In first step, target blood sample are...

N-15 (Nitrogen-15) NMR studies of the widely-used coupling agent 3-aminopropyltriethoxysilane (APS) deposited onto native-oxide alumina and silica in submonolayers from nonaqueous aqueous solutions reveal at least four different APS nitrogen environments. When is on solvents IPA (isopropanol) NMP (N-methylpyrrolidone), three environments, which are believed to be amine, 'closed form' unique form, present. However, when water, only two amine cation seen. silica, closed form all present...

The study focuses on the range of pigments and media that Italian artist Lucio Fontana (1899–1968) employed for selected works dating between 1949 1968. Samples were taken from ten groups works, all canvas support, as well painting equipment survived in artist's former two studios. Techniques analysis used pyrolysis-gas chromatography–mass spectrometry identification, laser desorption ionization time-of-flight mass Raman microspectroscopy pigment identification. Fontana's reputation a daring...

This study investigates the efficacy of a sampling technique to acquire sufficient sample for peptide mass fingerprinting analysis (PMF) with minimal alteration photograph surfaces. The technique, which is potentially useful surface coatings in wide variety situations, uses very fine polishing film (1–30 μm particles, 14,000–600 grit) abrade and remove small amounts material consistent PMF requirements. Several variations devices were evaluated using coated salt print mock-ups collection...

Identification and characterization of ancient proteins still require technical developments towards non-invasiveness, sensitivity, versatility ease use the analyses. We report that enzyme functionalized films, described in Cicatiello et al. (2018), can be used efficiently on surface different objects ranging from fixative-coated paper to canvas coating an albumen photograph, as well much harder surfaces ivory proteinaceous binders decoration a wooden Egyptian coffin. The mixture digested...

Abstract Accelerated ageing is an often-used tool in conservation research, yet difficult to apply parchment due the materials inherent heat sensitivity. The complexity of this organic material makes analysis its deterioration especially challenging. An attempt develop a non-heat protocol for described paper. work was performed within scope MA-thesis at State Academy Art and Design Stuttgart. Samples modern new limed goat pieces reconstituted made from bovine hide powder were subjected...

Mark Rothko's Harvard Murals are a series of paintings on canvas commissioned by University and created in the early 1960s. The present study investigates creative process, materials, technique with ultraviolet infrared imaging, analytical chemistry, cross-sectional analysis, comparisons literature. Infrared Raman spectroscopy imaging cross sections help chemical mapping paint stratifications. A comparison paper studies, studies canvas, final five works installed give detailed insight into...

The site of Iita in northwestern Greenland had near-continual occupation from 1000 CE to the mid-twentieth century, which presents an unparalleled opportunity examine diachronic changes use marine mammals for food and raw material resources by Pre-Inuit (Late Dorset) Inuit–Inughuit—two culturally distinct Indigenous groups. Limited wood High Arctic environments necessitated antler, bone, ivory tool making. Choices selection processing osseous reflect way these two groups used same resources....

The mass spectrometric properties of a series model ion pairs were examined. In the cases studied it was possible to vaporize pair consituents and produce spectra corresponding those unpaired materials. These findings offer convenient means for derivatizing certain ionic compounds demonstrate feasibility analyzing species by on-line liquid chromatography-mass spectrometry.

Abstract A miniaturized, integrated capillary electrophoresis‐ultraviolet detection‐electrospray ionization‐mass spectrometry (CE‐UV‐ESI‐MS) interface has been constructed and evaluated. The device incorporates a fiber optic detection cell close to the electrospray tip allow UV monitoring of separated zones just prior their admittance into mass spectrometer. This configuration provides precise information about time when UV‐active enter allows easy location analyte in ion current profile....

Abstract Esters of 2‐diazonaphthalenone sulfonic acid were examined by mass spectrometry using electron impact and chemical ionization techniques. Charactristic differences in the fragmentation patterns positional isomers have been observed. In a manner analogous to photo‐induced decompositions, diazoketo functional groups fragment elimination N 2 form an indenoketene ion. An alternative process involves apparent loss 26 units from molecular The latter is explained evoking abstraction two...

A survey of graphite drawings by the English painter John Constable revealed that in many cases artist applied a coating overtop media. This thin, clear, glossy was characterized on over thirty where it appeared to be same material although employed different ways. Several attempts were made identify with minimal success until peptide mass fingerprinting (PMF) used, and minimally invasive sampling method involving vinyl eraser cubes proved very easy effective technique collect sufficient...

Abstract This paper details the investigation of a discrete coating observed on group Egyptian panel paintings, six mummy portraits and one funerary panel, dating from first-third century CE. Six in this are encaustic, is tempera using an animal glue binder. An accretion or has been surface recesses paint layers these panels. Examination ultraviolet radiation revealed irregular visible fluorescence surface. On portraits, often extends only as far where linen wrappings would have secured...