Several methods of Deoxyribonucleic acid (DNA) extraction have been applied to extract bacterial DNA. The amount and the quality DNA obtained for each one those are variable. study aimed evaluate using conventional boiling method followed by alcohol precipitation. from Gram negative bacilli was extracted precipitated with further precipitation ethanol. procedure performed resulted in high yields both E. coli K. pneumoniae bacteria (199.7 285.7μg/ml, respectively) which close control (229.3...

BACKGROUND: Cross contamination of contact surfaces in slaughterhouses and meat shops (butcheries) may contain antibiotic resistance genes that pose a serious public health challenge. AIMS: The present study aimed to assess prevalence Gram-negative strains isolated from at shops. METHODS: A total 71 swab samples were collected different cultured for bacterial identification, testing, gene detection.  Polymerase chain reaction (PCR) was used detect strA-strB, sul2, aadA1, tet(B),...

The emergence of OXA-type beta-lactamases has become a significant threat to public healthcare systems and may lead prolonged hospital stays increased mortality rates among affected patients. This study aimed determine the prevalence oxacillinase resistance (OXA) genes in multidrug-resistant (MDR) Gram-negative bacteria. One hundred six clinical isolates were collected from stock identified tested for antibiotic susceptibility presence OXA using polymerase chain reaction (PCR). most common...

This study aimed to assess hand hygiene knowledge, perception, and practices of visitors the Prophet’s Mosque in Al Madinah City, Saudi Arabia. Using a self-administered electronic questionnaire, cross-sectional survey was conducted among domestic residents, who visited mosque between 31 July 3 August 2020. Participants’ demographic data, were collected. Four hundred participants aged 18–65 (median 36) years completed survey, which 215 (53.8%) female. The visitors’ mean knowledge score about...

Background: Multidrug-resistant Pseudomonas aeruginosa has become a hazard to public health, making medical treatment challenging and ineffective. Whole-genome sequencing for antibiotic susceptibility testing offers powerful replacement conventional microbiological methods. Objective: The present study evaluated the presence of resistance genes in selected clinical strains P. using whole-genome testing. Results: susceptible common antibiotics showed 4 gene types, fosA, catB7, blaPAO,...

Methicillin-resistant

Abstract To control infectious diseases, various applications of nanotechnology have been used to enhance the self-cleaning and antibacterial properties materials . This study aimed evaluate face masks coated with TiO2 nanoparticles The efficacies cloth were measured by inoculating them in bacterial suspensions (10 5 CFUs from both E. coli S. aureus ). results showed that nanoparticle solutions (at 2%) reduced starting inoculum 10 (5 log cfu/cm 2 ) 1.3 1.68 log, respectively, activities 3.7...

Since the peak of coronavirus disease 2019 (COVID-19) pandemic, concerns around multidrug-resistant (MDR) bacterial pathogens have increased. This study aimed to characterize aminoglycoside resistance genes in MDR Klebsiella pneumonia e ( K . pneumoniae ) collected during COVID-19 pandemic. A total 220 clinical isolates gram-negative bacteria were from tertiary hospitals Makkah, Saudi Arabia, between April 2020 and January 2021. The prevalence was 40.5%; 89 isolates, patterns found among 51...

Aminoglycosides are the most frequently prescribed antimicrobial agents in Saudi Arabia; they routinely used for treatment of gram-negative bacillary infections. The aim this study was to detect resistance patterns against different aminoglycoside antibiotics and prevalence genes encoding Pseudomonas aeruginosa isolates from Hera General Hospital, Makkah, KSA. All that were resistant one or more antibiotic subjected susceptibility test PCR analysis presence genes: aac (6')-Ib, aac(3)-Ia,...

Food handlers who carry enterotoxin-producing Staphylococcus aureus could become potential reservoirs of Staphylococcal food poisoning. The study is a cross-sectional one aimed to determine the prevalence methicillin-resistant S. (MRSA) and staphylococcal enterotoxins from randomly selected in Al Jazirah state, Sudan. Culture swabs were collected hands nasals (2016-2018). Identification was done on basis conventional laboratory tests. All isolates screened for MRSA enterotoxin (SE) genes by...

The acceleration of inactivating viable cells Escherichia coli (E. coli), by using new direct and indirect innovative methods, is the targeted method an atmospheric pressure plasma jet (APPJ) operated AC high-voltage power source with variable frequency up to 60 kHz voltage ranging from 2.5 25 kV. Discharges dry argon (0% O2) discharges different wet admixtures O2/Ar ratios 0.25% 1.5% were studied. combined effects discharges, exposure a mesh controller, hollow magnets studied reach complete...

Pseudomonas aeruginosa (P.aeruginosa) has been reported as the most commonly isolated organisms in hospitals.The increasing resistance rate of P. to common antimicrobial drugs worldwide.The present study aimed investigate incidence and antibiotic susceptibility pattern from inpatients two hospitals.Out 1235 patient specimens, a total 108 (8.7%) non-duplicated clinical isolates were identified, majority them males (59.3%) patients above 60 years (31.5%).The was male ward (43.5%) followed by...

Bacterial conjunctivitis has worldwide distribution, affecting persons of all ages, races, social strataand both genders. The present study aimed to isolate and identify the common bacteria causing their antibiotic susceptibility in Khartoum state, Sudan. Conjunctival swabs were collected from 200 patients attended Eye Hospital allwaldin Hospital. directly inoculated onto blood agar, MacConkey agar chocolate then a smear was prepared each swab. For C. trachomatis identification based on...

Hospitalized patients are likely to have chronic illnesses and at an increased risk of mortality due infection caused by MDR bacteria. We aimed identify carbapenem-resistant genes carrying Klebsiella pneumoniae (K. pneumoniae) isolates their factors recovered from adult with comorbidities. A cross-sectional study was carried out between April 2021 December King Abdullah Hospital (KAH) in Bisha province, Saudi Arabia. Seventy-one multi-drug resistant K. clinical samples screened for...

Three hundred and thirty two (n=332) urine specimens were collected from patients attending different hospitals in KhartoumState during the period May to November 2011. The isolates transported microbiology lab (research lab) at SudanUniversity for Sciences Technology. Identification of was done by using conventional biochemical methods microbact 2000 24E (OXOID) system. All Isolates then stored –70°C Tryptic Soy broth with 20% glycerol. isolated bacteria tested their antimicrobial...

Mass gathering events commonly encounter environmental challenges that necessitate assurance of water quality and food security. The current outbreak the coronavirus disease 2019 (COVID-19) call for maintaining safe drinking supply providing assessment tools to avoid contamination in sources or distribution networks. Arid conditions also add more stress on supplied mass events. Herein, we assess (desalinated 95% groundwater 5%) Makkah city, Saudi Arabia during a event (9.6 million people)...

Acceleration of antibacterial properties is the targeted fashion recent part our project by studying different techniques, on culture media E. coli., including non-thermal effect using atmospheric pressure plasma jet (APPJ) and effects combined with photocatalytic APPJ coupled a titanium dioxide TiO2 precursor. The electrical, non-thermal, optical characteristics laminar turbulent mode flow dry argon discharge afterglow were vital in study properties, measured as follows: frequency 25 kHz;...

The quality of drinking water is an important health issue in crowded cities, so that adequate with good must be provided, hence over hundreds coolers are distributed Makkah city to cope the increasing demand for water. present study aimed determine chemical and microbial from city. Sixty-three samples randomly selected were tested bacteriological quality. For all samples, mean value physiochemical tests pH (7.12), TDS (152.7 ppm), turbidity (2.56 NTU), free chlorine (0.312 fluoride (0.112...