A5056420383- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Travel-related health issues
- Viral Infections and Vectors
- Urinary Tract Infections Management
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Vaccine Coverage and Hesitancy
- Influenza Virus Research Studies
- Plasma Applications and Diagnostics
- Legume Nitrogen Fixing Symbiosis
- Plasma Diagnostics and Applications
- COVID-19 Clinical Research Studies
- Bacterial Genetics and Biotechnology
- COVID-19 and healthcare impacts
- Plant Pathogenic Bacteria Studies
- Electrohydrodynamics and Fluid Dynamics
- Pneumonia and Respiratory Infections
- Genomic variations and chromosomal abnormalities
- Legionella and Acanthamoeba research
- Laser-Ablation Synthesis of Nanoparticles
- Polymer Nanocomposite Synthesis and Irradiation
The Wistar Institute
2025
Umm al-Qura University
2010-2024
University of Sheffield
2001-2016
Assiut University
2014
Shura Council
2005
Arabian Gulf University
1998
Family and Social Services Administration
1994
Methicillin-resistant S. aureus (MRSA) tends to be resistant multiple antibiotics. Methicillin resistance is conferred by the acquisition of mecA gene, which carried a mobile genetic element called staphylococcal cassette chromosome mec (SCCmec). There are five major types SCCmec elements (I-V). The majority hospital-acquired MRSA (HA-MRSA) strains carry I, II, or III, whereas community-acquired (CA-MRSA) IV V. In addition, Panton-Valentine Leucocidin (PVL) gene encoding powerful cytotoxin...
BACKGROUND: Cross contamination of contact surfaces in slaughterhouses and meat shops (butcheries) may contain antibiotic resistance genes that pose a serious public health challenge. AIMS: The present study aimed to assess prevalence Gram-negative strains isolated from at shops. METHODS: A total 71 swab samples were collected different cultured for bacterial identification, testing, gene detection.  Polymerase chain reaction (PCR) was used detect strA-strB, sul2, aadA1, tet(B),...
Transcription-replication conflicts frequently occur at repetitive DNA elements involved in genome maintenance functions. The KSHV terminal repeats (TR) function as the viral episome element when bound by encoded nuclear antigen LANA. Here, we show that transcription-replication or near LANA binding sites TR. We proximity ligation assay (PLA) PCNA and RNAPII colocalize with LANA-nuclear bodies (LANA-NBs). Using DNA-RNA-IP (DRIP) assays S9.6 antibody, demonstrate R-loops form find these are...
This study aims to bio-modulate Poly (vinyl alcohol) crosslinked by silver and gold nanoparticles fabricated one-step laser ablation. The optical, cell viability, antibacterial characterization of the films have been studied via different techniques. FTIR XRD were used investigate molecular structures polymer matrix Polyvinyl Alcohol incorporating (Ag-Au NPs) created data illustrate semicrystalline structure PVA, with two hump peaks at 2θ = 8.52° 20.17° that are decreased when loaded Ag-Au...
Since the peak of coronavirus disease 2019 (COVID-19) pandemic, concerns around multidrug-resistant (MDR) bacterial pathogens have increased. This study aimed to characterize aminoglycoside resistance genes in MDR Klebsiella pneumonia e ( K . pneumoniae ) collected during COVID-19 pandemic. A total 220 clinical isolates gram-negative bacteria were from tertiary hospitals Makkah, Saudi Arabia, between April 2020 and January 2021. The prevalence was 40.5%; 89 isolates, patterns found among 51...
Introduction: The World Health Organization’s persistent reporting of global outbreaks influenza A viruses, including the 2009 pandemic swine H1N1 strain (H1N1pdm09), justified targeted surveillance pilgrims during their annual congregation that pools more than two million people from around 165 nations in a confined area Makkah city Kingdom Saudi Arabia (KSA). Methodology: total 1,600 were included and Hajj (pilgrimage) season 2010. Each pilgrim responded to demographic health...
To elucidate the function of a gene in bacteria it is vital that targeted inactivation (allelic replacement) can be achieved. Allelic replacement often carried out by disruption interest insertion an antibiotic-resistance marker followed subsequent transfer mutant allele to genome host organism place wild-type gene. However, due their intrinsic resistance many antibiotics only selected markers used members genus Burkholderia, including Burkholderia cepacia complex (Bcc). Here we describe...
The opportunistic pathogen Burkholderia cenocepacia produces the siderophores ornibactin and pyochelin under iron-restricted conditions. Biosynthesis of both requires involvement non-ribosomal peptide synthetases (NRPSs). Using a transposon containing lacZ reporter gene, two B. mutants were isolated which deficient in siderophore production. Mutant IW10 was shown to produce normal amounts but only trace pyochelin, whereas synthesis abolished AHA27. Growth AHA27, not IW10, inhibited In...
Gram-positive bacteria are important nosocomial pathogens. The objective of this study was to estimate the frequencies and resistance rates gram-positive pathogens isolated from hospitals in Makkah, Saudi Arabia.Prospective at three Makkah May 2008 April 2009.Clinical isolates were collected demographic laboratory data recorded. Standard microbiological methods used identify organisms test for antimicrobial susceptibility. results interpreted according Clinical Laboratory Standards Institute...
This study describes a seroepidemiological survey on Rift Valley fever (RVF) among small ruminants and their close human contacts in Makkah, Saudi Arabia. A total of 500 (126 local, 374 imported) were randomly selected from the sacrifice livestock yards Al-Kaakiah slaughterhouse, holy city during pilgrimage season 1432 H (4–9 November 2011). In addition, blood samples collected 100 local workers contact with animals at slaughterhouse. An RVF competition multi-species enzyme-linked...
Aminoglycosides are the most frequently prescribed antimicrobial agents in Saudi Arabia; they routinely used for treatment of gram-negative bacillary infections. The aim this study was to detect resistance patterns against different aminoglycoside antibiotics and prevalence genes encoding Pseudomonas aeruginosa isolates from Hera General Hospital, Makkah, KSA. All that were resistant one or more antibiotic subjected susceptibility test PCR analysis presence genes: aac (6')-Ib, aac(3)-Ia,...
The uptake of the pneumococcal vaccine is suboptimal in Australia and remains unknown among Australian Hajj pilgrims, many whom are eligible because age or underlying disease at particular risk travel activities Hajj. Pneumococcal vaccination was examined over three consecutive years (2011 to 2013) through anonymous self-administered cross sectional surveys pilgrims who assembled Mina valley, Mecca, Saudi Arabia. Respectively, 158, 513 219 were recruited 2011, 2012 2013; their mean ages 43.8...
The acceleration of inactivating viable cells Escherichia coli (E. coli), by using new direct and indirect innovative methods, is the targeted method an atmospheric pressure plasma jet (APPJ) operated AC high-voltage power source with variable frequency up to 60 kHz voltage ranging from 2.5 25 kV. Discharges dry argon (0% O2) discharges different wet admixtures O2/Ar ratios 0.25% 1.5% were studied. combined effects discharges, exposure a mesh controller, hollow magnets studied reach complete...
<i>Objective:</i> To determine the prevalence of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) strains among clinical isolates collected from 4 tertiary hospitals in Makkah, Saudi Arabia, and to test antimicrobial susceptibility patterns <i>S. against 9 agents. <i>Materials Methods:</i> A total 512 were during a period 1 year starting April 2003 Al-Noor, King Abdul-Aziz, Hera Faisal Hospitals, Arabia. The sensitivity these determined...
The ferric uptake regulator (Fur) functions as a transcription repressor of many genes in bacteria response to iron, but the presence functional equivalent this protein has not been demonstrated Burkholderia cepacia. A segment pseudomallei fur gene was amplified using degenerate primers and used identify chromosomal restriction fragments containing entire B. cepacia pseudomallei. These were cloned sequenced, revealing Fur both species be polypeptide 142 amino acids possessing high degree...
Dry argon (Ar) discharge and wet oxygen/argon (O2/Ar) admixture for alternating current atmospheric pressure plasma jets (APPJs) were studied Ar discharges with flow rates ranging from 0.2 to 4 slm O2/Ar different O2 ratios 2.5 15 mslm. The voltage–current waveform signals of APPJ discharge, gas rate, photo-imaging the jet length width, power, axial temperature distribution, optical emission spectra, irradiance investigated. Different behavior varying oxygen content in was observed....
Three hundred and thirty two (n=332) urine specimens were collected from patients attending different hospitals in KhartoumState during the period May to November 2011. The isolates transported microbiology lab (research lab) at SudanUniversity for Sciences Technology. Identification of was done by using conventional biochemical methods microbact 2000 24E (OXOID) system. All Isolates then stored –70°C Tryptic Soy broth with 20% glycerol. isolated bacteria tested their antimicrobial...