A5020378021- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- Retinal Diseases and Treatments
- Hormonal Regulation and Hypertension
- Genomics, phytochemicals, and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Chemokine receptors and signaling
- Stress Responses and Cortisol
- Herpesvirus Infections and Treatments
- Ocular Oncology and Treatments
- Bone Tissue Engineering Materials
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Developmental Biology and Gene Regulation
- Fibroblast Growth Factor Research
- CAR-T cell therapy research
- Vitamin C and Antioxidants Research
- Photoacoustic and Ultrasonic Imaging
- Mitochondrial Function and Pathology
- Vascular Tumors and Angiosarcomas
- Alzheimer's disease research and treatments
- Global Financial Crisis and Policies
- Adrenal Hormones and Disorders
- CRISPR and Genetic Engineering
University of Washington
2008-2024
VA Puget Sound Health Care System
2024
University of North Carolina at Chapel Hill
2012-2017
Institut des Sciences Cognitives Marc Jeannerod
2016
National Institutes of Health
2008-2013
National Eye Institute
2008-2013
Office of the Director
2009
Purpose Microglia represent the primary resident immune cells in CNS, and have been implicated pathology of neurodegenerative diseases. Under basal or "resting" conditions, microglia possess ramified morphologies exhibit dynamic surveying movements their processes. Despite prominence this phenomenon, function regulation microglial morphology behavior are incompletely understood. We investigate here whether how neurotransmission regulates behavior. Methods employed an ex vivo mouse retinal...
purpose. Retinal microglia have been implicated in the pathogenesis of various retinal diseases, but their basic function and cellular phenotype remain incompletely understood. Here, authors used a novel ex vivo imaging preparation to examine behavioral living intact tissue response injury. methods. Fluorescence-labeled explants from CX3CR1+/GFP transgenic mice were observed using time-lapse confocal imaging. High spatial temporal resolution parameters follow dynamic microglial behavior real...
Microglia in the central nervous system display a marked structural dynamism their processes resting state. This dynamic behavior, which may play constitutive surveying role uninjured neural parenchyma, is also highly responsive to tissue injury. The of CX3CR1, chemokine receptor expressed microglia, regulating microglia morphology and behavior state after laser-induced focal injury was examined.Time-lapse confocal imaging retinal explants used evaluate labeled with green fluorescent protein...
Many adeno-associated virus (AAV) serotypes efficiently transduce the retina when delivered to subretinal space but show limited success vitreous due inner limiting membrane (ILM). Subretinal delivery of AAV serotype 2 (AAV2) and its heparan sulfate (HS)-binding-deficient capsid led similar expression, indicating transduction outer occurred by HS-independent mechanisms. However, intravitreal HS-ablated recombinant AAV2 (rAAV2) a 300-fold decrease in compared AAV2. Fluorescence situ...
Abstract The Notch signaling pathway is a promising target for controlling cell fate choices at the biomaterial‐tissue interface. Building on our previous work in developing Notch‐signaling biomaterials, we evaluated various immobilization schemes ligands, and their effect human foreskin keratinocytes. A peptide sequence derived from Jagged‐1 DSL‐region immobilized to poly(2‐hydroxyethyl methacrylate) (polyHEMA) showed no bioactivity relation Notch‐CSL pathway. full‐length protein directly...
NRF2 is a transcription factor that drives antioxidant gene expression in multiple organ systems. We hypothesized Nrf2 overexpression could be therapeutically applied toward diseases which redox homeostasis disrupted. In this study, adeno-associated virus (AAV)-Nrf2 was tested mouse model of acute acetaminophen-induced liver toxicity and successfully conferred protection from hepatotoxicity, validating the vector design early onset NRF2-mediated protection. Furthermore, therapeutic potential...
Objective Perivascular mural cells of the choroid have been implicated in physiological functioning as well retinal disease pathogenesis. However details regarding their form and function are not understood. We aim to characterize choroidal adult mouse terms distribution morphology, correlate these contractile behavior. Methods Sclerochoroidal flat-mounted explants were prepared from albino transgenic mice which α-smooth muscle actin (α-SMA) promoter drives expression green fluorescent...
Abstract Context Many studies have moved toward saliva and peripheral blood sampling for studying cortisol, even in relation to disorders of the brain. However, degree which cortisol reflects central levels has yet be comprehensively described. Data describing effect that biological characteristics such as age sex on across compartments is also limited. Objective To assess relationships cerebrospinal fluid (CSF), saliva, plasma (total free) describe effects these relationships. Design...