Women accessing the public health system in Gauteng province, South Africa are largely unscreened for cervical cancer and have a high background prevalence of human immunodeficiency virus (HIV) infection.This cross-sectional study describes age-specific papillomavirus (HPV) infection cytological abnormalities among this urban peri-urban population.Over period March 2009 - September 2011, 1 524 women attending sector primary healthcare clinics were invited to participate screening study. All...

Objective To assess the performance of APTIMA ® HPV E6/E7 mRNA assay (AHPV) with 16 and 18/45 genotyping (AHPV-GT) cytology in detecting cervical cancer precancer HIV positive negative women South Africa. Methods A multicentre cross-sectional study was performed aged 25–64 (n = 992) AHPV AHPV-GT reflex testing. All screen-positive a random subset screen-negative were referred for colposcopy biopsy. Results On cytology, low-grade squamous intraepithelial lesion (LSIL) or higher found 9.7%...

Abstract Background Cervical cancer screening strategies should ideally be informed by population-specific data. Strategies recommended for secondary prevention, are often inadequately studied in populations with high cervical disease burdens. This report describes the test performance measured against CIN2 + /CIN3 histology HIV-positive women (HPW) and HIV-negative (HNW) aim to determine most effective identify South African at risk. Methods Primary using visual inspection, cytology HPV DNA...

Background: To evaluate the prevalence of human papilloma virus (HPV) infection and types in oral cervix mucosa treatment‐naïve HIV‐1‐positive women with CD4 counts less than 300 cells per ml no HPV‐associated lesions. Methods: Oral epithelium was harvested from buccal lateral borders tongue cervical samples were collected endocervical area 30 women, 22–64 years old. Cytobrush Plus cell collectors used for sampling both anatomical areas. Genital pathology, obstetric gynaecological history,...

Background. Human papillomavirus (HPV) infection is well known to be associated with head and neck cancers (HNCs). HPV-associated HNCs are related sexual behaviour, particularly the lifetime number of oral sex partners, but epidemiology oropharyngeal HPV in South African men has not yet been studied. Objectives. To determine strain prevalence factors a selected male population Pretoria, Africa (SA). Methods. Male factory workers were recruited. Oral rinse gargle samples tested for 37 types...

To determine the performance of molecular screening strategies for detection cervical intraepithelial neoplasia grade 3 or worse (CIN3+) in comparison with cytology women living HIV.Post-hoc analysis using data from a South African study cohort.Cytology and human papillomavirus (HPV)-based were evaluated, including single test FAM19A4/miR124-2 methylation triage strategies. Participants underwent colposcopy-directed biopsy. Valid results on cytology, HPV status, 16/18 genotyping histology...

Cervical cancer is the most common among women in Sub-Saharan Africa. treatable if detected timeously, yet only 20% of South African have ever been for a Pap smear their lifetime due to limited access screening, transport or child care responsibilities.To evaluate acceptability self-collection cervical screening. We aimed identify which device prefer and they would consider using them routine screening.HIV-positive (>18 years) from urban rural HIV clinics were interviewed following an...

<h3>Objectives</h3> Cervical cancer is the most common cause of cancer-related deaths among South African women. Viral types associated with cervical may differ not only between countries and regions, but possibly also human immunodeficiency virus (HIV)–infected noninfected <h3>Methods</h3> In a population high HIV prevalence, papillomavirus (HPV)–type infections detected DNA analyses were reported in cohort 299 women diagnosed invasive cancer. <h3>Results</h3> One hundred fifty-four tested...

To evaluate the performance of hypermethylation analysis ASCL1, LHX8 and ST6GALNAC5 in physician-taken cervical scrapes for detection cancer intraepithelial neoplasia (CIN) grade 3 women living with HIV (WLHIV) South Africa.Samples from a prospective observational cohort study were used these analyses. Two cohorts included: WLHIV who invited screening (n = 321) gynaecologic outpatient referred evaluation abnormal cytology or biopsy proven 108, 60% seropositive). Cervical collected all...

Cervical cancer is the leading cause of cancer-related death in women South Africa. This study evaluates DNA methylation levels cervical (pre)cancer and aims to assess value high-risk human papillomavirus (hrHPV) testing analysis, alone or combination, on physician-taken scrapes detect cancer, intraepithelial neoplasia grade 3 (CIN3) an HIV-infected African population.Prospective observational multicentre cohort study.Women from a living with HIV (n = 355) referral 109, 60% seropositive)...

The platform provided by human papillomavirus (HPV) vaccination for linked public health interventions to improve cervical cancer prevention remains incompletely explored. Vaccine And Cervical Cancer Screen (VACCS) cross-sectional observation trials aimed evaluate the efficacy of school-based HPV with maternal screening.Girls from 29 schools in two provinces South Africa were invited writing receive vaccination. Two approaches informed consent compared, namely an audiovisual presentation...

Human papillomavirus (HPV)-based primary screening guidelines are based on test performance and prevalence data generated in high-resource areas with low HIV infection rates. There is an urgent need for local disease prevalence, as well performance, among both HIV-positive HIV-negative South African (SA) women, order to inform updated guidelines. Objectives. This study describes the baseline characteristics of participants cross-sectional phase multicentric DIAgnosis Vaccine And Cervical...

Cervical cancer is preventable and caused by persistent infection with oncogenic human papilloma virus (HPV) types. HPV screening more sensitive the preferred test. data are mainly from developed settings, purpose of this study was to investigate performance in previously unscreened HIV positive negative women.In cross sectional multicenter study, liquid based cytology testing were performed on women attending different clinics. Patients tests had colposcopy biopsy or large loop excision...

Background. In Africa, data on the relationship between oncogenic human papillomavirus (HPV) types, immune status and cervical preinvasive lesions are lacking. Methods. We investigated low-risk (lrHPV) high-risk (hrHPV) HPV types in a cohort of women with intraepithelial neoplasia (CIN) II/III confirmed histological examination, an urban setting high prevalence HIV infection. Results. Of 270 CIN II/III, 45 were HIV-negative 225 HIV-positive. HIV-infected had significantly more type...

Occupational injuries in medical students are concerning, especially countries with a high prevalence of bloodborne infections. With more HIV-infected patients on antiretroviral treatment, appropriate post-exposure prophylaxis (PEP) depends knowledge source patients' infection status and treatment response. This study determined the number type exposure incidents, reporting practices, PEP use among at University Pretoria, South Africa.Data were collected from an anonymous voluntary...

Abstract Background Compared with women who are human immunodeficiency virus (HIV) negative, (WWH) have a higher papillomavirus (HPV) prevalence and increased cervical cancer risk, emphasizing the need for effective screening in this population. The present study aimed to validate methylation markers ASCL1 LHX8 primary South African cohort of WWH. Methods In post hoc analysis within DIAgnosis Vaccine And Cervical Cancer Screen (DiaVACCS) study, observational multicenter scrape samples from...

Background: Cervical cancer prevention in regions with limited access to screening and HPV vaccination necessitates innovative approaches. This study explored the potential of a test-and-treat strategy using mRNA tests impact cervical high-prevalence HIV population. Methods: A was conducted at three South African hospitals involving 710 under-screened, non-pregnant women (25 65 years) without known diseases. Cytology, testing, colposcopy, biopsies were performed concurrently....

Multipurpose vaccines (MPVs) could be formulated to prevent multiple sexually transmitted infections simultaneously. Little is known about acceptability of MPVs among vaccine health care providers (HCPs) or mothers adolescent girls. 151 and 118 girls aged 9–14 were recruited from five geographically-diverse countries: Argentina, Malaysia, South Africa, Korea, Spain. We assessed providers' preferences for single-purpose human papillomavirus (HPV) versus (including HPV+herpes simplex virus...

The identification of human papillomavirus (HPV) as the aetiological agent for cervical cancer has important implications future screening and prevention strategies. Despite availability HPV vaccines, regular adequate will remain mainstay some time to come. Molecular tests high-risk DNA E6/E7 mRNA have potential improve in developed developing countries. latest international South African private sector guidelines propose incorporation molecular testing patient management, backed by good...