A5010212928- Cervical Cancer and HPV Research
- Reproductive tract infections research
- Urinary and Genital Oncology Studies
- Genital Health and Disease
- Global Cancer Incidence and Screening
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Vector-Borne Animal Diseases
Vrije Universiteit Amsterdam
2019-2022
Cancer Center Amsterdam
2022
Amsterdam University Medical Centers
2019-2022
Abstract In human papillomavirus (HPV) cervical cancer screening, cytology is used as triage to counter the low specificity of HPV testing. VALID‐SCREEN a EU‐multicenter, retrospective study conducted evaluate clinical performance FAM19A4/miR124‐2 methylation‐based molecular test substitute or addition reflex testing screen positive women. methylation (QIAsure Methylation Test) was evaluated in 2384 HPV‐positive screening samples, from women 29‐76 years age, derived four EU countries....
Widespread adoption of primary human papillomavirus (HPV)‐based screening has encouraged the search for a triage test which retains high sensitivity detection cervical cancer and precancer, but increases specificity to avoid overtreatment. Methylation analysis FAM19A4 miR124‐2 genes shown promise high‐risk (hr) HPV‐positive women. In our study, we assessed consistency / methylation in series 519 invasive carcinomas ( n = 314 scrapes, 205 tissue specimens) from over 25 countries, using...
High-grade cervical intraepithelial neoplasia (CIN2 and CIN3) represents a heterogeneous disease with varying cancer progression risks. Biomarkers indicative for productive human papillomavirus (HPV) infection (HPV E4) transforming HPV (p16ink4a , Ki-67 host-cell DNA methylation) could provide guidance clinical management in women high-grade CIN. This study evaluates the cumulative score of immunohistochemical expression p16ink4a (Scores 0-3) 0-3), referred to as "immunoscore" (IS), 262 CIN2...
Abstract Background The introduction of primary HPV screening has doubled the number colposcopy referrals because direct referral HPV-positive women with a borderline or mild dyskaryosis (BMD) cytology (ASC-US/LSIL) triage test. Further risk-stratification is warranted to improve efficiency HPV-based screening. Methods This study evaluated discriminative power FAM19A4/miR124-2 methylation, HPV16/18 genotyping and HPV16/18/31/33/45 in BMD ( n = 294) two Dutch trials. Absolute CIN3+ risks...
Abstract Background High-grade squamous intraepithelial lesions (HSIL) or cervical neoplasia (CIN) grade 2/3 in human papillomavirus (HPV)–positive women <30 years of age have high spontaneous regression rates. To reduce overtreatment, biomarkers are needed to delineate advanced CIN that require treatment. We analyzed the FAM19A4/miR124-2 methylation test and HPV16/18 genotyping HPV-positive aged years, aiming identify CIN2/3 need Methods A European multicenter retrospective study was...
Abstract Background Compared with women who are human immunodeficiency virus (HIV) negative, (WWH) have a higher papillomavirus (HPV) prevalence and increased cervical cancer risk, emphasizing the need for effective screening in this population. The present study aimed to validate methylation markers ASCL1 LHX8 primary South African cohort of WWH. Methods In post hoc analysis within DIAgnosis Vaccine And Cervical Cancer Screen (DiaVACCS) study, observational multicenter scrape samples from...
Women with HIV (WWH) have an increased risk to develop recurrent cervical intraepithelial neoplasia grade 2/3 (rCIN2/3) after treatment compared HIV-negative women. Therefore, appropriate posttreatment monitoring of WWH is important. This study evaluates the performance ASCL1 and LHX8 methylation analysis as test in treated for CIN2/3, alternative cytology or human papillomavirus (HPV) follow-up test.Prospective observational cohort study.WWH CIN2/3 by large loop excision transformation zone...