A5045858790- Cervical Cancer and HPV Research
- COVID-19 Clinical Research Studies
- Hepatitis B Virus Studies
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Global Cancer Incidence and Screening
- T-cell and Retrovirus Studies
- Genital Health and Disease
- Reproductive tract infections research
- Reproductive System and Pregnancy
- Intensive Care Unit Cognitive Disorders
- Viral Infections and Outbreaks Research
- Respiratory Support and Mechanisms
- Immune Response and Inflammation
- Endometrial and Cervical Cancer Treatments
- HIV/AIDS Impact and Responses
- HIV/AIDS Research and Interventions
- Pancreatic and Hepatic Oncology Research
- Herpesvirus Infections and Treatments
- Cytokine Signaling Pathways and Interactions
- Neuroendocrine Tumor Research Advances
- Lymphadenopathy Diagnosis and Analysis
- Heparin-Induced Thrombocytopenia and Thrombosis
- TGF-β signaling in diseases
- Diabetes Treatment and Management
Western Isles Hospital
2019-2022
University of Edinburgh
2016-2022
Dumfries and Galloway Royal Infirmary
2015-2021
National Health Service Scotland
2017-2019
University of Zimbabwe
2001-2007
Karolinska Institutet
2003-2007
Abstract In human papillomavirus (HPV) cervical cancer screening, cytology is used as triage to counter the low specificity of HPV testing. VALID‐SCREEN a EU‐multicenter, retrospective study conducted evaluate clinical performance FAM19A4/miR124‐2 methylation‐based molecular test substitute or addition reflex testing screen positive women. methylation (QIAsure Methylation Test) was evaluated in 2384 HPV‐positive screening samples, from women 29‐76 years age, derived four EU countries....
Papillomavirus Dumfries and Galloway (PaVDaG) assessed the performance of a high-risk human papillomavirus (hrHPV) PCR-based assay to detect high-grade cervical intraepithelial neoplasia (CIN2+) in self-collected vaginal urine samples.Women attending routine screening primary care.5318 women aged 20-60 years provided random samples for hrHPV testing clinician-collected liquid-based cytology (LBC) sample testing.HrHPV testing. All were tested using cobas 4800 assay. Colposcopy was offered...
The purpose of our prospective, case-controlled study was to investigate the hypothesis that women who are genetically programmed produce high or medium levels IL-10 were more likely develop cancer uterine cervix than individuals predisposed low production. population recruited from patients attending gynecological clinics at 2 hospitals in Harare, Zimbabwe. Laboratory tests performed Departments Immunology, Chemical Pathology and Medical Microbiology, School, University Zimbabwe,...
Abstract Background: Several options for the triage of high-risk HPV screen–positive (hrHPV+) women were assessed. Methods: This study incorporated CIN2+ cases and controls, all whom tested hrHPV+ whose results liquid-based cytology (LBC), HPV16/18 genotyping, p16/Ki67 cytoimmunochemistry available. Sensitivity specificity these tests evaluated. Results: Absolute sensitivities 16/18 typing, LBC, p16/Ki-67 detection 61.7%, 68.3%, 85.0% with clinician-taken samples. Respective specificities...
Background:The failure of specific types human papillomaviruses (HPV) to raise effective immune responses may be important in the pathogenesis cervical cancer, second most common cancer South African women.Polymorphisms a number cytokine genes have been implicated inducing susceptibility or resistance cancers caused by infectious agents owing their role determining host response.Polymorphisms IL-10 and IFN-γ are believed influence expression and/or secretion levels respective cytokines....
The purpose of the study was to investigate hypothesis that genetically programmed ability produce low, medium, or high levels tumor necrosis factor-alpha (TNF-alpha), as determined by TNF-alpha promoter polymorphism at position 308, influenced development cancer uterine cervix. population recruited from patients attending gynecological clinics two teaching hospitals in Harare, Zimbabwe. Laboratory tests were performed Departments Immunology and Medical Microbiology, School, University One...
To assess the performance of a clinically validated human papillomavirus (HPV) test (the Cobas 4800 HPV test) in urine and self-taken vaginal specimens within colposcopy population to prevalence before after treatment across different biospecimens.A total 100 women attending clinic provided three biospecimens (a clinician-taken liquid-based cytology sample (LBC), sample) for testing. concordance was compared clinical relative detection cervical intraepithelial neoplasia (CIN)2+ CIN3+...
Abstract Despite the high prevalence of both human papillomavirus (HPV) infections and cervical cancer among Zimbabwean women, ability to test for HPV infection uterine cervix is limited by a lack an easy sample collection method that does not require gynecological examination. The presence HPVs in urine swab samples collected from 43 women who presented with invasive was investigated. detection done means degenerate primers nested polymerase chain reaction (PCR). Typing using restriction...
Background. Cervical cancer affects 1 in 2000 Zimbabwean women. We investigated the type‐specific distribution of human papillomavirus (HPV) infection women with invasive cervical cancer. Methods. conducted a descriptive study on 98 The methods used were nested polymerase chain reaction (PCR) for amplification HPV‐DNA and restriction fragment length polymorphism (RFLP) to characterize HPV types. Results. was identified 97% cases. types 16, 33, 18 31 61%, 39%, 18% 4% patients, respectively....
Abstract Background: We evaluated the longitudinal performance of three options: HPV16/18 genotyping (HPV16/18), cytology (LBC), and p16/Ki-67 dual stain (DS) for triage high-risk Human Papillomavirus–positive (Hr-HPV+) women within cervical screening program in Scotland. Methods: Data were derived from a cohort Hr-HPV+ (n = 385) who participated PaVDaG (Papillomavirus Dumfries Galloway) study. Performance strategies detecting high-grade disease was assessed at 3 (in <50 years) or 5...
Summary Transforming growth factor‐beta 1 (TGF‐β1) has a multifactorial role in the development of cervical cancer. It potently inhibits epithelial cells that harbour oncogenic human papilloma viruses (HPVs). TGF‐β1 also expression early viral transforming regions E6 and E7, which appear to be key oncoproteins. been suggested squamous cell carcinomas are devoid TGF‐β1, raising possibility elevated levels this factor could protect against is recognized production genetically predetermined...
Self-sampling provides a powerful means to engage women in cervical screening. In the original Papillomavirus Dumfries and Galloway study (PaVDaG), we demonstrated cross-sectional similarity of high-risk human papillomavirus (Hr-HPV) testing on self-taken vaginal vs clinician-taken samples for detection intraepithelial neoplasia 2 or worse (CIN2+). Few data exist longitudinal performance self-sampling; present outcomes PaVDaG. Routinely screened provided clinician-collected sample....
Abstract Objectives. To investigate the hypothesis that women who are genetically programmed to produce higher levels of transforming growth factor‐beta 1 more likely develop severe eclampsia/pre‐eclampsia. Design. Case–control study. Methods. Blood samples from whose pregnancy was complicated by eclampsia ( n = 37) or pre‐eclampsia 49) and healthy controls 86) were analyzed for presence polymorphisms at codons 10 25 gene. The thought determine whether an individual produces low, medium,...
To establish the prevalence of detectable low-risk and high-risk, oncogenic HPV types in cervical swabs women with histologically proven cancer cervix.Cross sectional study.Harare Central Parirenyatwa Hospitals.119 whom 63 had degree differentiation tumour reported.Frequency infection high human papillomaviruses.The presence DNA was demonstrated 63% (75/119) cases. Low risk HPVs were present 26% (31/119) high-risk 51% (61/119) samples tested. Co-infection both observed 14% (17/119)...
Background. Cervical cancer affects 1 in 2000 Zimbabwean women. We investigated the type-specific distribution of human papillomavirus (HPV) infection women with invasive cervical cancer. Methods. conducted a descriptive study on 98 The methods used were nested polymerase chain reaction (PCR) for amplification HPV-DNA and restriction fragment length polymorphism (RFLP) to characterize HPV types. Results. was identified 97% cases. types 16, 33, 18 31 61%, 39%, 18% 4% patients, respectively....
The purpose of the study was to investigate hypothesis that genetically programmed ability produce low, medium, or high levels tumor necrosis factor-alpha (TNF-α), as determined by TNF-α promoter polymorphism at position 308, influenced development cancer uterine cervix. population recruited from patients attending gynecological clinics two teaching hospitals in Harare, Zimbabwe. Laboratory tests were performed Departments Immunology and Medical Microbiology, School, University One hundred...
<h3>Objectives</h3> The optimal triage of screen-positive women is one the most important considerations in HPV cervical screening. Performing liquid based cytology (LBC) on HPV-positive (HPV+) and/or limited genotyping has been recommended as a strategy. Additionally p16/ki-67 dual-stained credible approach. We previously reported an evaluation strategies cross sectional study. aim this study was to evaluate cumulative incidence rate (CIR) CIN2+ within 5 years primary HPV+ women....