A5008045175- Complement system in diseases
- Receptor Mechanisms and Signaling
- Advanced Drug Delivery Systems
- Adenosine and Purinergic Signaling
- Neuropeptides and Animal Physiology
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Nanoparticle-Based Drug Delivery
- Bioinformatics and Genomic Networks
- Immune Response and Inflammation
- Reproductive System and Pregnancy
- Gene expression and cancer classification
- Protein Hydrolysis and Bioactive Peptides
- Lipid Membrane Structure and Behavior
- Neuroscience and Neuropharmacology Research
- Amyotrophic Lateral Sclerosis Research
- Metabolomics and Mass Spectrometry Studies
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Pregnancy and Medication Impact
- Neurogenetic and Muscular Disorders Research
- biodegradable polymer synthesis and properties
- Immune Cell Function and Interaction
The University of Queensland
2017-2025
G-protein-coupled receptors (GPCRs), also known as seven transmembrane (7TMRs), typically interact with two distinct signal-transducers, i.e., G proteins and β-arrestins (βarrs). Interestingly, there are some non-canonical 7TMRs that lack protein coupling but βarrs, although an understanding of their transducer preference, downstream signaling, structural mechanism remains elusive. Here, we characterize such 7TMRs, namely, the decoy D6 receptor (D6R) complement C5a subtype 2 (C5aR2), in...
Impaired glucose regulation is increasingly recognised in amyotrophic lateral sclerosis (ALS), yet the precise mechanisms remain unclear. Here, we investigated energy balance and control TAR DNA-binding protein 43 (TDP-43)Q331K mice, a model of ALS, at both early late symptomatic stages disease. Mutant TDP-43Q331K mice non-transgenic controls underwent indirect calorimetry, as well intraperitoneal glucose, insulin, glucagon tolerance testing. We also examined plasma hormone levels quantified...
BACKGROUND As a substitute for traditional drug therapy, digital cognitive-behavioral therapy positively impacts the regulation of brain function, which can improve insomnia. However, there is currently paucity studies on cognitive behavioral as treatment AIM To assess insomnia regarding its positive impact function. METHODS Participants were randomly assigned to either go/no-go group or dot-probe group. The primary outcome was quality sleep assessed by actigraphy monitoring bracelet,...
The anaphylatoxin C5a is a complement peptide associated with immune-related disorders. binds equal potency to two GPCRs, C5aR1 and C5aR2. Multiple agonists have been developed interrogate the receptor function but none show selectivity for C5aR1. To address these limitations, we potent stable that display no C5aR2 activity over 1000-fold C3aR. This includes BM213, which induces C5aR1-mediated calcium mobilization pERK1/2 signaling not β-arrestin recruitment, BM221, exhibits bias. Both...
The molecular links between sterile inflammation and induction of adaptive immunity have not been fully identified. Here, we examine how damage-associated patterns (DAMPs), as opposed to pathogen-associated molecules (PAMPs), regulate the immune response non-self-antigens presented at site a physical injury. Heat applied briefly skin invokes inflammation, characterized by local cell death caspase-1 activation without demonstrably disrupting integrity. Co-delivery ovalbumin (OVA) with heat...
Abstract Substantial preclinical data have validated cyclic hexapeptide complement C5a receptor 1 antagonists (C5aRAs) that target immune cells, as novel therapies for a range of inflammatory diseases currently limited effective treatment options. However, like most small‐molecule peptides, their poor oral bioavailability and short circulation half‐life are major hurdles clinical translation. Here, single emulsion technique is employed to produce poly(lactic‐ co ‐glycolic) acid nanoparticles...
Peptides hold promise as therapeutics, they have high bioactivity and specificity, good aqueous solubility, low toxicity. However, typically suffer from short circulation half-lives in the body. To address this issue, here, we developed a method for encapsulation of an innate-immune targeted hexapeptide into nanoparticles using safe non-toxic FDA-approved materials. Peptide-loaded were formulated two-stage microfluidic chip. Microfluidic-related factors (i.e., flow rate, organic solvent,...
The complement C5a receptor 1 (C5aR1) has been studied as a potential therapeutic target for autoimmune and inflammatory diseases, with several drug candidates identified. Understanding the pharmacokinetics pharmacodynamics of candidate is crucial preclinical step that allows greater understanding compound's in vivo biodistribution engagement to assist clinical dose selection dosing frequency. However, few pharmacodynamic methods have described inhibitors. In this study, we, therefore,...
Abstract The ability to spatially measure multi-modal data provides an unprecedented opportunity comprehensively explore molecular regulation at transcriptional, translational and metabolic levels acquire insights on cellular activities underpinning health disease. However, there is currently a lack of analytical tools integrate complementary information across different spatial-omics modalities, particularly with respect spatial metabolomics data, which becoming increasingly invaluable. We...
The complement activation peptide C5a is a key mediator of inflammation that associated with numerous immune disorders. binds and activates two seven-transmembrane receptors, C5aR1 C5aR2. Experimentally, utilized to investigate receptor biology screen for potential C5aR1/C5aR2 therapeutics. Currently, laboratory sources stem from either isolation endogenous human serum or most predominantly via recombinant expression. An alternative approach production chemical synthesis, which has several...
Abstract G protein-coupled receptors (GPCRs) are typically characterized by their seven transmembrane (7TM) architecture, and interaction with two universal signal-transducers namely, the heterotrimeric G-proteins β-arrestins (βarrs). Synthetic ligands receptor mutants have been designed to elicit transducer-coupling preferences distinct downstream signaling outcomes for many GPCRs. This raises question if some naturally-occurring 7TMRs may selectively engage one of these signal-transducers,...
The poor oral bioavailability and short circulation half-life of peptides are major hurdles that limit their clinical translation. Here, a single emulsion technique is employed to produce peptide PMX205 loaded poly(lactic-co-glycolic) acid nanoparticles. In article number 2200109, Trent M. Woodruff, Felicity Y. Han, co-workers demonstrate in mice the reformulated increases its therapeutic potential.