Amphotericin B (AMB) is used most commonly in severe systemic life-threatening fungal infections. There currently an unmet need for efficacious formulation amenable to oral administration with better bioavailability and lower nephrotoxicity. Novel PEGylated polylactic-polyglycolic acid copolymer (PLGA-PEG) nanoparticles (NPs) formulations of AMB were therefore studied their ability kill Candida albicans (C. albicans). The antifungal activity was assessed C. albicans. Its bioavalability...

This study aims to prepare, characterize and evaluate the pharmacokinetics of liposomal donepezil HCl (LDH) dispersed into thiolated chitosan hydrogel (TCH) in rabbits. Various hydrogels including TCH were prepared, after characterization, was selected for subsequent evaluations, due promising results. then incorporated with LDH prepared by reverse phase evaporation method. The characterized using scanning electron microscope, dialysis membrane technique, ultra-performance liquid...

Amphotericin B (AmB) is an effective anti-fungal and anti-leishmanial agent. However, AmB has low oral bioavailability (0.3%) adverse effects (e.g., nephrotoxicity). The objectives of this study were to improve the by entrapping in pegylated (PEG) poly lactide co glycolide copolymer (PLGA-PEG) nanoparticles (NPs). feasibility different surfactants stabilizers on mean particle size (MPS) entrapment efficiency also investigated.NPs prepared a modified emulsification diffusion method employing...

Cyclodextrin nanosponges are solid nanoparticles, designed by cross-linking of cyclodextrin polymer; it has been used widely as a good delivery system for water insoluble drugs. The aim this study is to enhance the solubility Piroxicam (PXM) using β-Cyclodextrin based formulations. PXM nanosponge (PXM-NS) formulations were prepared β-cyclodextrin and carbonyldiimidazole cross linker, three ratios crosslinker in addition drug tested. characterized its particle size, zeta potential, physical...

Aims: This study aimed to enhance the bioavailability and therapeutic efficacy of lamotrigine (LMG), an antiepileptic drug with low solubility, by formulating it into a nasal nanoemulsion (NE) for effective epilepsy control. Methods: LMG was incorporated (LMG-NE) using 32 factorial design via spontaneous emulsification method. LMG-NEs were characterised loading (DL), entrapment efficiency (EE%), particle size, microscopic examination, rheological profile, phosphatidylcholine liposome uptake,...

Poly(ethylcyanoacrylate) (PECA) nanospheres have been employed as biodegradable polymeric carriers for oral (po) delivery of insulin. The main goal this investigation was to screen various absorption enhancers, which were used protect insulin-loaded PECA, by following their in vivo performance after administrations streptozotocin-induced diabetic rats. prepared polymerization a continuous aqueous phase at pH 2.5 and the presence Pluronic 68 (0.5%). This technique able hold 85 #45 7.5%...

Inflammatory bowel disease (IBD), consists of two primary types: Ulcerative Colitis (UC) and Crohn's Disease (CD). Infliximab (IFX) Adalimumab (ADA) are frequently utilized in the management moderate to severe cases IBD.

ABSTRACT Warfarin is routinely monitored by assessing its pharmacologic effects on the international normalized ratio. However, having a patient with INR not responding to increasing warfarin dose mandates direct measurement of concentrations (total and free) for better clinical management therapy. Therefore, new fully validated specific, precise accurate ultra‐performance liquid chromatography tandem mass spectrometry was developed determination free total in human plasma. Free measured...

Abstract A new, precise, simple and accurate HPLC method was developed for the first time to separate determine mebeverine enantiomers. Enantiomeric resolution achieved on a cellulose Tris (3,5‐dimethylphenyl carbamate) column known as Chiralcel OD, with UV detection at 263 nm. The mobile phase consisted of n ‐hexane, isopropyl alcohol triethylamine (90:9.9:0.1 v/v/v). Sample run 18 min. On using chromatographic conditions described, enantiomers were well resolved mean retention times about...

AbstractTheophylline nanoparticles were prepared by emulsifier-free emulsion polymerization technique in continuos aqueous phase. The process was carried out at a pH 3. Different concentrations of isobutylcyacoacrylate (IBCA) used to investigate the effect monomer concentration. vitro release theophylline phosphate buffer studied. An HPLC assay follow drug from nanospheres. This able hold 2349% initially dissolved medium. percentage loading is concentration dependent. Increasing above 40 μL...

Abstract A simple, specific and accurate high performance liquid chromatographic (HPLC) method for determination of tramadol in pharmaceutical dosage forms has been developed. Reversed phase chromatography was conducted using (.μ-Bondapak C18 column (3.9 × 150 nm) with an isocratic mobile consisting 0.005 M triethylamine 0.01 sodium phosphate buffer (pH 5.5) containing 17% acetonitrile. The effluent monitored on a UV detector at 230 nm. Each analysis required no longer than 8 minutes....

The efficacy and pharmacokinetics of antimony were explored in 12 young male patients with cutaneous leishmaniasis following intramuscular administration sodium stibogluconate equivalent to 600 mg (Sb). Patients' cure rate was evaluated up 6 weeks after treatment. Blood samples collected at different time periods on the first last days a 3-week Twenty-four-hour urine also both occasions for estimation renal clearance (CL(r)). blood concentrations Sb profile best described by 2-compartment...

Amphotericin B (AmB) is the first-line agent for treatment of life-threatening invasive fungal infections. The aim this study was to monitor AmB in critically ill Saudi patients ICU after i.v. administration 0.68 ± 0.1 mg/kg/day Fungizone®. A selective, sensitive and precise UPLC MS/MS method developed measure concentrations these patients. Seven with creatinine clearance (ClCr) >40 mL/min were included. levels analyzed using a Waters Aquity system, BEH Shield RP18 column detection via...

Abstract A simple, sensitive, and reproducible HPLC method has been developed for the determination of metoclopramide employing reversed phase high performance liquid chromatography with UV detection at 270 nm. The separation was performed on a Novapak C18, 4 μm (3.9 × 150 mm) column. Acetonitrile (18%) in 0.02 M ammonium acetate containing 0.1% triethylamine used as mobile run time 7 min. Tramadol internal standard. mean retention times tramadol were 3.4 4.6 min, respectively. Linear...

Polyisobutylcyanoacrylate (PIBCA) nanospheres were employed as biodegradable polymeric carriers for oral (p.o.) and subcutaneous (s.c.) delivery of insulin. The polymerization technique used was able to hold 65%-95% insulin added 30 min after initiation polymerization. percentage drug loading monomer concentration dependent. Insulin adsorption the measured by radioimmunoassay. Although Pluronic F68 (0.5%) did not significantly alter in vitro degradation half-life T50%, sodium cholate...

Objectives: The efficacy of ketorolac tromethamine (KT) floating alginate beads as a drug delivery system for better control KT release was investigated. formulation with the highest loading, entrapment efficiency, swelling, buoyancy, and in vitro would be selected further vivo analgesic effect mice pharmacokinetics study rats compared to tablet dosage form.Methods: were prepared by extrusion congealing technique. plasma samples analyzed using UPLC MS/MS assay.Results: percentage yield,...

The purpose of this study was to develop and assess the in vitro characteristics carbamazepine-loaded microspheres. A solvent evaporation method used incorporate carbamazepine (CBZ) into poly (D,L-lactide-co-glycolide) (PLGA) with different molecular weights. optimum conditions for CBZ-PLGA microspheres preparation were considered release CBZ PLGA followed up 24 hr USP dissolution medium. effect using ratios microspheres, prepared weights, optimizing also investigated. encapsulation...

Flurbiprofen (FL) is a chiral 2-arylpropionate used clinically as the racemate (rac-FL). This study was undertaken to investigate influence of sustained release formulation on pharmacokinetics flurbiprofen enantiomers (-) -R-FL and (+)-S-FL. Therefore, stereoselective high-performance liquid chromatographic (HPLC) method developed validated for rapid, quantitative determination (-)-R-FL (+)-S-FL in rat plasma. Flurbiprofen-loaded poly(D,L-lactide-co-glycolide) nanoparticles (rac-FL-PLGA)...

Abstract A 1, 4‐dihydropyridine ⇌ pyridinium salt type redox system is described as a general and flexible method for site‐specific sustained delivery of drugs to the brain. This concept was used in present investigation model deliver an alkylating antitumor agent into bis‐(chloroethyl)amine drug hooked chemical (CDS) through amide linkage. Five newtarget compounds ( 23—27 ) CDS were synthesized reduction five new quaternary intermediates 18—22 ). The 4‐dihydropyridines subjected various...