A5062691728- Phytoestrogen effects and research
- Cholesterol and Lipid Metabolism
- Atherosclerosis and Cardiovascular Diseases
- Botanical Research and Chemistry
- MicroRNA in disease regulation
- Fatty Acid Research and Health
- Protein Hydrolysis and Bioactive Peptides
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- HER2/EGFR in Cancer Research
- Plant and fungal interactions
- Drug Solubulity and Delivery Systems
- Biochemical and Structural Characterization
- Pluripotent Stem Cells Research
- Circular RNAs in diseases
- Galectins and Cancer Biology
- Protein Interaction Studies and Fluorescence Analysis
- Nuclear Receptors and Signaling
- Systemic Lupus Erythematosus Research
- Lipid metabolism and biosynthesis
- Soybean genetics and cultivation
- GDF15 and Related Biomarkers
University of Milan
2011-2023
Pharmac
2008
The aim of the present study was to evaluate effect a chronic oral γ-conglutin treatment in male Sprague–Dawley rats which hyperglycaemia had been induced by supplying 10 % d -glucose drinking-water. A dosage 28 mg/kg body weight daily administered animals for 21 d. Plasma glucose, insulin and glucose overloading were monitored. Chronic administration resulted statistically significant ( P < 0·05) increase fasting blood (2·5-fold) (2·7-fold) v. values recorded control rats. Simultaneous...
Cigarette smoke (CS) is a risk factor for inflammatory diseases, such as atherosclerosis. CS condensate (CSC) contains lipophilic components that may represent systemic cardiac factor. To better understand CSC effects, we incubated mouse and human aortic smooth muscle cells (SMCs) with CSC. We evaluated specific markers contractile [i.e., actin, (ACTA2), calponin-1 (CNN1), the Kruppel-like 4 (KLF4), myocardin (MYOCD) genes] IL-1β, IL-6, IL-8, galectin-3 (LGALS-3) phenotypes. increased...
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin growth family, well known for its role in homeostasis cardiovascular system. Recently, human BDNF Val66Met single nucleotide polymorphism has been associated with increased propensity arterial thrombosis related to acute myocardial infarction (AMI). Using cardiac magnetic resonance imaging and immunohistochemistry analyses, we showed that homozygous mice carrying (BDNFMet/Met) undergoing left anterior descending (LAD)...
A promising approach for the development of high-affinity tumor targeting ADCs is use engineered protein drugs, such as affibody molecules, which represent a valuable alternative to monoclonal antibodies (mAbs) in cancer-targeted therapy. We developed method more efficient purification ZHER2:2891DCS conjugated with cytotoxic antimitotic agent auristatin E (MMAE), and its efficacy was tested vitro on cell viability, proliferation, migration, apoptosis. The effects ZHER2:2891DCS-MMAE were...
7S soy globulin and its alpha’ subunit have been shown to positively modulate LDL receptor activity decrease triglyceride synthesis both in vitro vivo . The aim of the study was evaluate effect a short chain (S‐alpha’) from globulin, on cell cholesterol homeostasis. HepG2 cells were incubated presence/absence (3.5.10 −6 M) or S‐alpha’ at concentration 10 −5 M M. A dose‐dependent increase up‐regulation LDL‐receptors has detected exposed vs that found controls. Moreover, 125 I‐LDL uptake (+...
The data presented in this article is related to the research entitled "ABCA1 and HDL3 are Required Modulate Smooth Muscle Cells Phenotypic Switch after Cholesterol Loading" (Castiglioni et al., 2017) [1]. This describes characterization of phenotypic changes induced by cholesterol loading smooth muscle cells (SMCs) isolated from aortae C57BL/6 mice. Upon loading, there a significant concentration-dependent decrease expression Acta2 parallel increase Mac-2, ATP binding cassette (ABC)...
Drug-eluting nanoparticles (NPs) administered by an eluting balloon represent a novel tool to prevent restenosis after angioplasty, even if the selection of suitable drug and biodegradable material is still matter debate. Herein, we provide proof concept use obtained combining grafting caffeic acid or resveratrol on poly(lactide-co-glycolide) backbone (g-CA-PLGA g-RV-PLGA) pleiotropic effects fluvastatin chosen because its low lipophilic profile which challenging for encapsulation in NPs...
Structural properties, and especially the differential stability, of complexes between carbonic anhydrase (CA) three sulfonamide inhibitors, acetazolamide, dorzolamide methazolamide, were investigated by spectroscopic electrophoretic techniques. These included denaturant gradient gel electrophoresis either across a urea or steady-state transverse sodium dodecyl sulfate (SDS) gradient. Acetazolamide, smallest most hydrophilic sulfonamides, forms stable complex in presence urea, whereas...
The aim of the present investigation was to evaluate effect a synthetic peptide, corresponding sequence which differs between alfa and alfa’ subunits from 7S soy globulin, on cell cholesterol homeostasis both in HepG2 cells rats fed casein-cholesterol diet. Hep G2 were incubated for 24 hr minimum essential medium (MEM) + 5% LPDS presence/absence whole globulin (0.5 mg/mL) or its subunit (0.125 peptide (10-5 M 10-6 M). Cell preincubation with caused an increase mature forms SREBP-1, membranes...
Cigarette smoke induces endothelial cell (EC) dysfunction. Circulating monocytes (HM) adhere to a damaged endothelium and migrate into the intima, differentiate macrophages contribute atherosclerotic plaque growth. We demonstrated that HM exposed cigarette condensate (CSC) increase transmigration of through EC. Thus, we hypothesized CSC may affect HM/EC interaction. analyzed effects on EC (30 μg/ml) alone and/or medium conditioned by incubated without (CM) or with (CM/CSC). CSC, CM CM/CSC...
Cells of the artery wall may accumulate free cholesterol and cholesteryl esters becoming foam cells. Up to 50% cells in human lesions originates from smooth muscle (SMCs). Arterial SMCs express ATP binding cassette (ABC) transporter ABCA1 and, upon loading, macrophage markers a phagocytic activity. To characterize role HDL3 this transdifferentiation process, we evaluated phenotypic changes isolated wild type (WT) knock out (KO) mice how affects these changes. Cholesterol loading causes...
Aim of the study was to evaluate effect a chronic oral administration conglutin γ, lupin seed protein, in male Sprague Dawley rats which hyperglicemia induced by drinking water containing 10% d‐glucose. A dosage 100 mg/kg b.w. γ administered daily for 21 days. Conglutin statistically significant reduction plasma glucose (‐25%) and triglyceride (‐20%) levels as well glycemia after loading, comparable that metformin. In order assess mechanism underlining this biological effect, Hep G2 cells,...
β-cyclodextrin (β-CD), an enzymatically modified starch, displays lipid lowering activity in rodents. In particular, the hydroxypropyl derivative (HP-β-CD) seems to affect cholesterol biosynthesis. order evaluate changes plasma and liver lipids as well lipoprotein distribution, both compounds were tested rats fed on a standard or hypercholesterolemic (HC) diet. β-CD (10 25 mg/kg b.w.) HP-β-CD (100 450 daily administered by gavage for 14 days. On diet, lowered total triglycerides (TG) (−25...
Aim: Smooth muscle cells (SMCs) phenotypic switching results in less-differentiated forms that lack SMC markers but show an increased inflammatory profile thus directly promoting atherosclerosis. We have recently demonstrated HDL3 may prevent cholesterol-induced switch ABCA1-dependent manner. Cigarette smoke is a risk factor for atherosclerosis and modulates also plasticity. To characterize the role of ABCA1 HDL 3 this process, we evaluated effects on cigarette condensate (CSC)-induced...