A5061035446- Ion Transport and Channel Regulation
- Renal function and acid-base balance
- Electrolyte and hormonal disorders
- Diet and metabolism studies
- Parathyroid Disorders and Treatments
- Metabolism and Genetic Disorders
- Immune cells in cancer
- Amino Acid Enzymes and Metabolism
- Fibroblast Growth Factor Research
- LGBTQ Health, Identity, and Policy
- Renal and related cancers
- Acute Kidney Injury Research
- Respiratory viral infections research
- Macrophage Migration Inhibitory Factor
- Erythrocyte Function and Pathophysiology
- Nitric Oxide and Endothelin Effects
- Cancer, Hypoxia, and Metabolism
- Reproductive Health and Technologies
- Pneumonia and Respiratory Infections
- Lymphatic System and Diseases
- Chemotherapy-induced organ toxicity mitigation
- Chronic Kidney Disease and Diabetes
- Immunodeficiency and Autoimmune Disorders
- Hematopoietic Stem Cell Transplantation
- Biomedical Research and Pathophysiology
National Center for Emerging and Zoonotic Infectious Diseases
2024
Centers for Disease Control and Prevention
2024
University of Alabama at Birmingham
2020-2023
Pediatric Nephrology of Alabama
2021
Florida College
2013-2019
University of Florida
2013-2019
Fibroblast growth factor (FGF) 23 is a phosphate-regulating hormone that elevated in patients with chronic kidney disease and associated cardiovascular mortality. Experimental studies showed FGF23 levels induce cardiac hypertrophy by targeting myocytes via FGF receptor isoform 4 (FGFR4). A recent structural analysis revealed the complex of FGFR1, physiologic kidney, includes soluble α-klotho (klotho) heparin, which both act as co-factors for FGF23/FGFR1 signaling. Here, we investigated...
Renal ammonia metabolism has a major role in the maintenance of acid-base homeostasis. Sex differences are well recognized as an important biological variable many aspects renal function, including fluid and electrolyte metabolism. However, sex have not been previously reported. Therefore, purpose current study was to investigate We studied 4-mo-old wild-type C57BL/6 mice fed normal diet. Despite similar levels food intake, and, thus, protein which is primary determinant endogenous acid...
Cellular release of lactate dehydrogenase (LDH) is being used as an injury marker; however, the exact localization LDH within nephron remains unclear. We show that isoform A expressed proximally, whereas B distally. Both subunit expressions were significantly altered in models acute kidney and chronic disease. Our study provides new insights into basal postinjury renal metabolism.
The mechanisms regulating proximal tubule ammonia metabolism are incompletely understood. present study addressed the role of basolateral electrogenic Na + -coupled bicarbonate cotransporter (NBCe1; Slc4a4) in renal metabolism. We used mice with heterozygous and homozygous NBCe1 gene deletion compared these their wild-type littermates. Because causes 100% mortality before day 25, we studied at 8 (±1 day). Both caused a dose-related decrease serum bicarbonate. ability to lower urinary pH was...
Renal ammonia metabolism is the primary mechanism through which kidneys maintain acid-base homeostasis, but molecular mechanisms regulating renal generation are unclear. In these studies, we evaluated role of proximal tubule basolateral plasma membrane electrogenic sodium bicarbonate cotransporter 1 variant A (NBCe1-A) in this process. Deletion NBCe1-A gene caused severe spontaneous metabolic acidosis mice. Despite acidosis, normally causes a dramatic increase excretion, absolute urinary...
Glutamine synthetase (GS) catalyzes the recycling of NH4 (+) with glutamate to form glutamine. GS is highly expressed in renal proximal tubule (PT), suggesting ammonia via could decrease net ammoniagenesis and thereby limit available for acid excretion. The purpose present study was determine role PT metabolism under basal conditions during metabolic acidosis. We generated mice PT-specific deletion (PT-GS-KO) using Cre-loxP techniques. Under conditions, PT-GS-KO increased urinary excretion...
The ammonia transporter family member, Rh B Glycoprotein (RhBG/Rhbg), is essential for transport by the rodent kidney, but in human kidney mRNA not protein expression has been reported. Because fundamental acid-base homeostasis, current study addressed RhBG kidney. Two distinct sequences have reported, with different numbers of consecutive cytosines at nt1265 and thus encoding carboxy-tails. Sequencing region difference both liver showed eight sequential cytosines, seven as some reports....
Dietary protein restriction has multiple benefits in kidney disease. Because intake is a major determinant of endogenous acid production, it important that net excretion change parallel during restriction. Ammonia the primary component excretion, and inappropriate ammonia can lead to negative nitrogen balance. Accordingly, we examined response then determined molecular mechanism changes observed. Wild-type C57Bl/6 mice fed 20% diet changed 6% developed an 85% reduction within 2 days, which...
Regulated dicarboxylate transport is critical for acid-base homeostasis, prevention of calcium nephrolithiasis, regulation collecting duct sodium chloride transport, and the blood pressure. Although luminal reabsorption via NaDC1 (SLC13A2) believed to be primary mechanism regulating renal specific localization in human kidney currently unknown. This study's purpose was determine NaDC1's expression normal neoplastic kidneys. Immunoblot analysis demonstrated with an apparent molecular weight...
Citrate is critical for acid-base homeostasis and to prevent calcium nephrolithiasis. Both metabolic acidosis hypokalemia decrease citrate excretion increase expression of Na+-dicarboxylate cotransporter 1 (NaDC1; SLC13A2), the primary protein involved in reabsorption. However, mechanisms transducing extracellular signals mediating these responses are incompletely understood. The purpose present study was determine role Na+-coupled electrogenic bicarbonate (NBCe1) A variant (NBCe1-A)...
Expansion of renal lymphatic networks, or lymphangiogenesis (LA), is well recognized during development and now being implicated in kidney diseases. Although LA associated with multiple pathological conditions, very little known about its role acute injury. The purpose this study was to evaluate the a model cisplatin-induced nephrotoxicity. predominately regulated by vascular endothelial growth factor (VEGF)-C VEGF-D, ligands that exert their function through cognate receptor VEGF 3...
The bicarbonate transporter, NBCe1 (SLC4A4), is necessary for at least two components of the proximal tubule contribution to acid-base homeostasis, filtered reabsorption, and ammonia metabolism. This study's purpose was determine NBCe1's role in a third component organic anion metabolism, by studying mice with deletion. Because deletion causes metabolic acidosis, we also examined acid-loaded wild-type adult if effects were specific or non-specific effect associated acidosis. Both KO...
Dietary protein restriction has multiple benefits in kidney disease. Because intake is a major determinant of endogenous acid production, it important that net excretion changes parallel during dietary intake. decreases production and urinary ammonia excretion, component excretion. Glutamine synthetase (GS) catalyzes the reaction [Formula: see text] glutamate, which regenerates essential amino glutamine generation. renal proximal tubule GS expression increases restriction, this could...
Abstract Background The Centers for Disease Control and Prevention’s Active Bacterial Core surveillance (ABCs) identified increased serotype 4 invasive pneumococcal disease (IPD), particularly among adults experiencing homelessness (AEH). Methods We quantified IPD cases during 2016-2022. Employing genomic-based characterization of isolates, we serotype-switch variants. Recombinational analyses were used to identify the genetic donor recipient strains that generated a progeny strain....
In acute kidney injury, macrophages play a major role in regulating inflammation. Classically activated (M1) undergo drastic metabolic reprogramming during their differentiation and upregulate the aerobic glycolysis pathway to fulfill pro-inflammatory functions. NAD+ regeneration is crucial for maintenance of most direct by which this occurs via fermentation pyruvate lactate, catalyzed lactate dehydrogenase A (LDHA). Our previous study determined that LDHA predominantly expressed proximal...
The mechanisms through which the kidneys increase ammonia generation in response to increased endogenous acid loads have been extensively studied; underlying decreased metabolism are less well understood. Glutamine synthetase is expressed kidney, and mediates reaction of NH 4 + with glutamate, generating glutamine decreasing net generation. Our previous studies show that dietary protein restriction, decreases production, increases proximal tubule expression. purpose current was determine...
Metabolic acidosis typically increases ammonia excretion, but people with proximal renal tubular (pRTA), despite acidosis, do not have increased excretion. Genetic forms of human pRTA involve the tubule bicarbonate transporter, NBCe1. This study's purpose was to determine NBCe1's role in metabolism. We used previously reported mice NBCe1 deletion. Because -/- 100% mortality between d10-21, we studied at d8±1. Serum HCO3 26.4±1.0 wild type (WT), 19.8±1.9 heterozygous (het), and 10.3±0.6 mM...
The proximal tubule electrogenic, sodium‐coupled bicarbonate transporter, NBCe1, has a critical role in two acid‐base functions, reabsorption and ammonia metabolism. A third component of homeostasis involves organic anion purpose the current study was to determine NBCe1's metabolism by comparing mice with NBCe1 deletion wild‐type (WT) littermates. Because KO causes early post‐natal mortality, we studied at day 8±1. Also, because metabolic acidosis, compared effects that experimental acidosis...
Lu, Yan; Zmijewska, Anna A.; Cheung, Matthew D.; Jiang, Yanlin; Traylor, Amie; Osis, Gunars; Agarwal, Anupam Author Information
The A‐splice variant of Na + ‐dependent, electrogenic bicarbonate transporter‐1 (SLC4A4, NBCe1‐A) is generally believed to be the only SLC4A4 isoform expressed in mammalian proximal tubule, and have critical roles filtered reabsorption, tubule ammonia metabolism organic anion metabolism. purpose current study was examine expression other splice variants mouse kidney. We used recently generated mice with TALEN‐generated deletion A D NBCe1 splice‐variants, but intact B, C E isoforms. RT‐PCR...
Osis, Gunars; Traylor, Amie; Eckenrode, Hannah; Black, Laurence M.; Cochrun, Steven L.; Agarwal, Anupam Author Information
Kentrup, Dominik; Yanucil, Christopher; Osis, Gunars; Campos, Isaac D.; Czaya, Brian A.; Heitman, Kylie; Faul, Christian Author Information
Eckenrode, Hannah; Gutierrez, Orlando M.; Osis, Gunars; Agarwal, Anupam; Curtis, Lisa M. Author Information