A5064952654- Biochemical and Molecular Research
- Enzyme Structure and Function
- Trypanosoma species research and implications
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- HIV/AIDS drug development and treatment
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Biochemical Acid Research Studies
- Research on Leishmaniasis Studies
- Chemical Reactions and Isotopes
- Venomous Animal Envenomation and Studies
- Metabolism and Genetic Disorders
- Toxin Mechanisms and Immunotoxins
- Metal-Catalyzed Oxygenation Mechanisms
- Chemistry and Chemical Engineering
- Bipolar Disorder and Treatment
- Microbial Metabolites in Food Biotechnology
- Galectins and Cancer Biology
- RNA modifications and cancer
- Synthesis and Biological Evaluation
- Adipose Tissue and Metabolism
- Polysaccharides and Plant Cell Walls
- Cancer Research and Treatments
Brazilian Center for Research in Energy and Materials
2017-2023
Brazilian Biosciences National Laboratory
2017-2023
Universidade de São Paulo
2008-2021
Universidade de Ribeirão Preto
2017-2021
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2020
SUPERA Park of Innovation and Technology of Ribeirão Preto
2010-2016
Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in de novo pyrimidine biosynthetic pathway and has been exploited as target for therapy against proliferative parasitic diseases. In this study, we report crystal structures of DHODH from Leishmania major, species associated with zoonotic cutaneous leishmaniasis, its apo form complex orotate fumarate molecules. Both were found to bind same active site exploit similar interactions, consistent a ping-pong mechanism described class 1A...
Abstract β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl aryl β-D-glucosides disaccharides. They rate-limiting reaction in conversion cellobiose to glucose saccharification cellulose for second-generation ethanol production, due this important role search tolerant enzymes is biochemical biotechnological importance. In study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl)...
A técnica de Termofluor constitui uma importante ferramenta na identificação moléculas protótipos a fármacos.No presente trabalho, foi desenvolvido um método alternativo para Termofluor, chamado ThermoFMN, que explora o grupo prostético flavina mononucleotídeo (FMN) como sonda fluorescente.A validação do feita através monitoramento da fluorescência FMN diferentes alvos macromoleculares presença biblioteca aleatória ligantes.Além disso, fármacos com eficácia comprovada tiveram seus perfis...
Effective use of plant biomass as an abundant and renewable feedstock for biofuel production biorefinery requires efficient enzymatic mobilization cell wall polymers. Knowledge composition architecture has been exploited to develop novel multifunctional enzymes with improved activity against lignocellulose, where a left-handed β–3-prism synthetic scaffold (BeSS) was designed insertion multiple protein domains at the prism vertices. This allowed construction series chimeras fusing variable...
Phosphate-activated glutaminases catalyze the deamidation of glutamine to glutamate and play key roles in several physiological pathological processes. In humans, GLS encodes two multidomain splicing isoforms: KGA GAC. both isoforms, canonical glutaminase domain is flanked by an N-terminal region that folded into EF-hand-like four-helix bundle. However, event replaces a well-structured three-repeat ankyrin with shorter, unordered C-terminal stretch The architecture, which contains putative...
Galectin-4 is a tandem-repeat-type galectin that expressed in the epithelium of alimentary tract from tongue to large intestine. Additionally, strong expression galectin-4 can also be induced cancers other tissues, including breast and liver. In order explore its potential as target for anticancer drug design, elucidation structural basis carbohydrate-binding specificities has been focused on. As an initial step, N-terminal carbohydrate-recognition domain human (hGal4-CRD-1) successfully...
Chlorocatechol 1,2-dioxygenase from the Gram-negative bacterium Pseudomonas putida (Pp 1,2-CCD) is considered to be an important biotechnological tool owing its ability process a broad spectrum of organic pollutants. In current work, crystallization, crystallographic characterization and phasing recombinant Pp 1,2-CCD enzyme are described. Reddish-brown crystals were obtained in presence polyethylene glycol magnesium acetate by utilizing vapour-diffusion technique sitting drops. Crystal...
Corticosteroid-binding globulin (CBG) is a blood plasma protein that transports the weakly water-soluble hormone, cortisol, throughout circulation.It member of serine protease inhibitor (SERPIN) structural family; upon cleavage by human neutrophil elastase, it undergoes canonical S-to-R transition, which results in change its binding affinity for allowing to release hormone at sites inflammation.We plan redesign CBG transport compounds other than physiological ligand and, harnessing them...
Cancer cells rely on different mechanisms to maintain their dysregulated metabolism and high proliferation. Metabolic rewiring with an increased dependency glutamine, the most abundant amino acid in blood, is a common feature of several types cancers, including triple-negative breast cancer (TNBC). The enzyme glutaminase (GLS) converts glutamine glutamate, reaction that provides α-ketoglutarate for tricarboxylic/citric cycle (TCA) chromatin-modifying enzymes, thus impacting proliferation...