A5028546941- Vector-borne infectious diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Insect and Pesticide Research
- Dermatological diseases and infestations
- Zoonotic diseases and public health
- Data-Driven Disease Surveillance
- Yersinia bacterium, plague, ectoparasites research
- Exercise and Physiological Responses
- Digital Imaging for Blood Diseases
- Bee Products Chemical Analysis
- Parasitic Infections and Diagnostics
- Vibrio bacteria research studies
- Parasites and Host Interactions
- Amoebic Infections and Treatments
- Suicide and Self-Harm Studies
- Biomedical Text Mining and Ontologies
Marshfield Clinic
2016-2025
University of Rochester Medical Center
2022
Marshfield Clinic
2013-2020
New York State Department of Health
2017
Medical College of Wisconsin
2017
Centers for Disease Control and Prevention
2008-2012
Metropolitan State University of Denver
2010
Vector (United States)
2009
University of California, Irvine
2009
National Center for Emerging and Zoonotic Infectious Diseases
2008-2009
ABSTRACT Anaplasmosis and ehrlichiosis are emerging tick-borne diseases with clinically similar presentations caused by closely related pathogens. Currently, laboratories rely predominantly on blood smear analysis (for the detection of intracellular morulae) serologic tests, both which have recognized limitations, for diagnostic purposes. We compared performance a published real-time PCR assay that incorporates melt curve to differentiate Anaplasma Ehrlichia species methods in an upper...
Babesiosis is an emerging tick-borne disease transmitted by the hard tick Ixodes scapularis, which also transmits Lyme disease. Better gradation of prognostic indicators are needed to determine patients may develop serious complications requiring hospitalization, and provide early guidance on appropriate therapy. In this study, we evaluated 128 with smear or real time polymerase chain reaction-confirmed Babesia microti infections over a period 16 years. Patients asplenia immunocompromising...
Abstract Background Lyme disease is the most common vector-borne in United States; however, its frequency not reliably measured through surveillance. Electronic health records (EHR) might capture and characteristics of cases more accurately. We queried EHR from 1 system to describe epidemiology Wisconsin during 2016–2019. Methods Within a cohort persons evaluated for disease, we applied case definition based on first-line antibiotics within 14 days diagnosis code or test order same day as...
Lyme disease (LD) is the most common vector-borne in United States, though traditional LD surveillance underestimates burden of disease. We validated algorithms for early localized and disseminated LD, with without LD-specific diagnosis codes, states high incidence their neighboring low incidence. identified cohorts potential incident cases administrative insurance claims data, October 2015-October 2023, 1 national regional insurer. Three were studied: a primary algorithm an code indicated...
Lyme disease, the most prevalent vector-borne disease in North America, is increasing incidence and geographic distribution as tick vector, Ixodes scapularis , spreads to new regions. We re-construct spatial-temporal invasion of human Midwestern US, a major focus transmission, from 1967 2018, analyse influence spatial factors on spread. A regression model indicates that three factors—proximity previously invaded county, forest cover adjacency river—collectively predict occurrence. Validation...
BACKGROUND The tick‐borne pathogen Babesia microti has become recognized as the leading infectious risk associated with blood transfusion in United States, yet no Food and Drug Administration–licensed screening tests are currently available to mitigate this risk. aim of study was evaluate performance an investigational enzyme immunoassay (EIA) for B. a test applied endemic nonendemic donor populations. STUDY DESIGN AND METHODS aimed 20,000 donors from areas States considered 10,000 area EIA....
Powassan virus (POWV) lineage II is an emerging tickborne flavivirus with unknown seroprevalence in humans. In a Lyme disease-endemic area, we examined the seroreactivity to POWV 2 patient cohorts and described clinical features of POWV-seroreactive patients. disease might be less neuroinvasive than previously thought.
Lyme disease (LD) is an increasing public health problem. Current laboratory testing insensitive in early infection, the stage at which appropriate treatment most effective preventing sequelae. The Disease Biobank (LDB) collects samples from individuals with symptoms consistent LD presenting or without erythema migrans (EM) annular, expanding skin lesion and uninfected areas of endemicity. Samples were collected 550 participants (298 cases 252 controls) according to institutional review...
Factors potentially contributing to the lower incidence of Lyme borreliosis (LB) in far-western than northeastern United States include tick host-seeking behavior resulting fewer human encounters, densities Borrelia burgdorferi-infected vector ticks peridomestic environments, and genetic variation among B. burgdorferi spirochetes which humans are exposed. We determined population structure over 200 infected nymphs primary bridging humans, Ixodes pacificus, collected Mendocino County, CA....
To evaluate the seasonal prevalence of human intestinal parasites in western states Colorado, Utah, New Mexico, and Montana, fecal samples were examined as part routine diagnostic testing from patients experiencing gastrointestinal discomfort August (summer) 2006, January (winter), April (spring) 2007. Parasite identification positive was confirmed using light microscopy after wet mount trichrome staining techniques. Seventy-eight 1,083 surveyed (7.2%) tested for at least 1 species parasite....
Human anaplasmosis (HA) is an emerging tick-borne disease that may present as a mild flu-like illness or life threatening, sepsis-like condition. Although severity hypothesized to relate immunopathology and immune dysfunction in humans, studies directly measure responses infected humans have been very limited. We quantified cytokines 80 confirmed HA patients using multiplex chemiluminescence immunoassay system compared similarly measured 1000 control subjects. Pro-inflammatory were...
The role of deer mice and other species Peromyscus as enzootic reservoirs for plague remains controversial. In this study, we evaluated early-phase vector efficiency Aetheca wagneri Baker, a common flea infesting mice, to determine the likelihood that Y. pestis could be spread mouse by species. We showed A. transmit bacteria laboratory early 3 d postinfection (p.i.), but transmission was quite low (1.03%; 95% CI: 0.19–3.34%) 1–4 p.i. compared with established Oropsylla montana Baker (10.63%;...
Approximately 100 cases of POWV disease were reported in the United States over past 10 years. Most have occurred Northeast (52) and Great Lakes (45) regions ( https://www.cdc.gov/powassan/statistics.html ). The prevalence ticks mammals is increasing, poses an increasing threat a greater geographical range. In areas Midwest where Lyme endemic, testing recommended for patients with recent tick bite, who been treated antibiotics, or exposure tested negative other tick-borne illnesses...
Adults with the tick-borne disease human granulocytic anaplasmosis (HGA) have a spectrum of acute febrile illnesses that, if untreated, might be severe. Clinical presentation and outcomes children HGA been poorly described.A retrospective analysis was conducted to determine frequency, presentation, pediatric patients between 1994 2015 in region Wisconsin which is highly endemic. Patients related International Classification Diseases Ninth Tenth Revision (ICD-9 ICD-10, respectively) codes or...
Abstract Background People with occupations that require them to spend time working outdoors in suitable tick habitats are predicted be at an increased risk for tick-borne diseases (TBDs). However, few studies have assessed the risks of outdoor employees United States. Methods We conducted a cross-sectional survey collect data on exposure ticks and TBD infections among U.S. Forest Service high incidence region northern Wisconsin, examine employee knowledge, attitudes, practices (KAPs)...
Background and objective Diagnosis of early Lyme disease (LD) often relies on clinical recognition the skin lesion, erythema migrans (EM), a diagnostic sign when laboratory testing is insensitive. Because EM can present in morphologically distinct forms, its by clinicians be challenging. This study aimed to characterize spectrum lesions patients presenting with suspected LD an ambulatory care setting identify features that might help better prepared recognize lesions. Methods Images from 69...
ABSTRACT In early Lyme disease (LD), serologic testing is insensitive and seroreactivity may reflect active or past infection. this study, we evaluated a novel assay for the direct detection of three species Borrelia spirochetes in whole blood. The T2 magnetic resonance (T2MR) platform was used to amplify DNA released from intact detect amplicon. Analytical sensitivity determined blood spiked with known concentrations spirochetes, assay's limit found be single-cell-per-milliliter range: 5...
Lyme disease is a well-recognized public health problem in the USA, however, other tick-borne diseases also have major impacts. Yet, limited research has evaluated changes spatial and temporal patterns of non-Lyme within endemic regions. Using laboratory data from large healthcare system north-central Wisconsin 2000–2016, we applied Kulldorf’s scan statistic to analyze spatial, seasonal clusters laboratory-positive cases human granulocytic anaplasmosis (HGA), babesiosis, ehrlichiosis at...
Socioeconomic indicators associated with temporal changes in the distribution of human plague cases New Mexico were investigated for 1976-2007. In 1980s, more likely census block groups poor housing conditions, but by 2000s, affluent areas concentrated Santa Fe-Albuquerque region.
Background.Powassan virus (POWV) is reported to cause a rare, life-threatening tick-borne encephalitis in North America.The transmitted by Ixodes scapularis, the same tick vector that transmits Lyme disease, anaplasmosis, and babesiosis.Given relatively high incidence of these other disease (TBD) infections some regions United States, it suspected with POWV may be underreported less severe or even asymptomatic infections.We evaluated evidence for exposure infection patients residing TBD...
Abstract Background: People with occupations that require them to spend time working outdoors in suitable tick habitats are predicted be at an increased risk for tick-borne diseases (TBDs). However, few studies have assessed the risks of outdoor employees. Methods: We conducted a cross-sectional survey evaluate exposure ticks and disease infections among U.S. Forest Service employees high TBD incidence region northwestern Wisconsin, examine their knowledge, attitudes, practices (KAPs)...