A5019435246- Vector-borne infectious diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Malaria Research and Control
- HIV Research and Treatment
- Dermatological diseases and infestations
- Glycosylation and Glycoproteins Research
- Wound Healing and Treatments
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- Tendon Structure and Treatment
- Erythrocyte Function and Pathophysiology
- Lymphoma Diagnosis and Treatment
- Cellular transport and secretion
- HIV/AIDS Research and Interventions
- Insect and Pesticide Research
- Virology and Viral Diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced Proteomics Techniques and Applications
- Invertebrate Immune Response Mechanisms
- Yersinia bacterium, plague, ectoparasites research
- Reproductive tract infections research
- Angiogenesis and VEGF in Cancer
- Protist diversity and phylogeny
Technical Products Incorporation (United States)
2024
Immunetics (United States)
1995-2018
University of California, Los Angeles
2013-2014
Cambridge Information Group
2012
New York Medical College
2008
University of Wisconsin–Madison
1989-1995
Assaf Harofeh Medical Center
1994
Tufts University
1992
Harvard University
1987-1988
The cause of Lyme disease, Borrelia burgdorferi, was discovered in 1983. A 2-tiered testing protocol established for serodiagnosis 1994, involving an enzyme immunoassay (EIA) or indirect fluorescence antibody, followed (if reactive) by immunoglobulin M and G Western immunoblots. These assays were prepared from whole-cell cultured B. lacking key vivo expressed antigens expressing that can bind non-Borrelia antibodies. Additional drawbacks, particular to the immunoblot component, include low...
Fibromodulin (FMOD) is an extracellular matrix (ECM) small leucine-rich proteoglycan (SLRP) that plays important role in cell fate determination. Previous studies revealed not only FMOD critical fetal-type scarless wound healing, but it also promotes adult closure and reduces scar formation. In addition, FMOD-deficient mice exhibit significantly reduced blood vessel regeneration granulation tissues during healing. this study, we investigated the effects of on angiogenesis, which event...
ABSTRACT The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted immunoblots without reduction either sensitivity specificity. In this study, costs 4 different testing strategies were compared using median charges these tests at 6 commercial diagnostic...
Fibromodulin (FMOD) plays a critical role in the wound-healing process. Our previous studies revealed that FMOD deficiency led to marked alterations adult wound healing characterized by delayed dermal cell migration, postponed closure, and increased scar formation, all accompanied impeded angiogenesis. Therefore, aim of this study was reveal effect on angiogenesis during process.In vivo angiogenic effects were assessed chick embryo chorioallantoic membrane assay, Matrigel (BD Bioscience,...
Erythema migrans, the most common manifestation of Lyme disease, has been associated with highly variable rates seropositivity for antibodies to Borrelia burgdorferi. Differences in sensitivities serologic assays detection these antibodies, however, may not be only or even primary explanation this observation. We investigated impacts four clinical variables on seropositivity--the duration erythema presence single versus multiple skin lesions, and gender age patient. In analysis, three...
Background Transfusion‐transmitted babesiosis caused by B abesia microti has emerged as a significant risk to the US blood supply. This study estimated prevalence of . antibodies in donors using an investigational enzyme immunoassay ( EIA ). Study Design and Methods A peptide‐based that detects both immunoglobulin Ig ) G IgM was developed validated. Donor samples randomly selected from areas defined high‐risk endemic, lower‐risk nonendemic for were deidentified tested Samples repeat reactive...
BACKGROUND The tick‐borne pathogen Babesia microti has become recognized as the leading infectious risk associated with blood transfusion in United States, yet no Food and Drug Administration–licensed screening tests are currently available to mitigate this risk. aim of study was evaluate performance an investigational enzyme immunoassay (EIA) for B. a test applied endemic nonendemic donor populations. STUDY DESIGN AND METHODS aimed 20,000 donors from areas States considered 10,000 area EIA....
A potential concern with any serologic test to detect antibodies Borrelia burgdorferi is whether the epitopes incorporated in provide sufficient cross-reactivity infection all of pathogenic strains species. This a particular for C6 test, which based on reactivity single peptide.C6 testing and 2-tier were performed acute-phase serum samples obtained from >158 patients erythema migrans whom genotype borrelial isolate was defined basis an analysis 16S-23S ribosomal DNA spacer region and/or...
Babesia microti is the foremost infectious risk to US blood supply for which a Food and Drug Administration (FDA)-licensed test unavailable donation screening. Characterization of antibody response B. correlation with parasitemia necessary guide screening donor management policies.During an FDA licensure trial, donors were prospectively screened (July-November 2013) using microti-specific enzyme immunoassay (EIA, Immunetics) in highly endemic (New York [NY]; n = 13,688), moderately...
Babesia is a tick-borne intraerythrocytic parasite that clinically and diagnostically similar to malaria parasite, conferring risk of misdiagnosis in areas where both parasites are endemic. Data on humans Africa lacking, despite evidence it present regional animal populations. Samples were collected November 2014 July 2015 Kilosa district, Tanzania, evaluated for infection. Clinical data laboratory samples (i.e., hemoglobin, rapid diagnostic testing [RDT] malaria, peripheral blood smear,...
ABSTRACT Although the current indirect immunofluorescent assay (IFA) diagnostic antibody test for human babesiosis is sensitive and specific, an immunoblot may be easier to standardize perform. Our objective, therefore, was determine efficacy of develop interpretive criteria diagnosing acute using a Babesia microti whole-cell lysate as antigen. We compared reactivity sera B. in 24 subjects experiencing symptoms expressing laboratory evidence babesiosis, 28 who experienced Lyme disease, 12...
Vaccine-induced seropositivity (VISP) or seroreactivity (VISR), defined as the reaction of antibodies elicited by HIV vaccines with antigens used in diagnostic immunoassays, can result reactive assay results for vaccinated but uninfected individuals, subsequent misclassification their infection status. The eventual licensure a vaccine will magnify this issue and calls development mitigating solutions advance. An immunoassay that discriminates between those has been developed to address...
AIMS--To evaluate the prevalence of antibodies to Chlamydia pneumoniae (TWAR) in relation other aetiological agents acute respiratory infections Israeli patients. METHOD--Serum samples from 604 patients (183 children and 421 adults) were collected over three years. Antibodies C pneumoniae, trachomatis, Legionella sp evaluated using microimmunofluorescence (MIF) assay. Mycoplasma detected Serodia Myco II test. RESULTS--Antibodies TWAR 319 (51.3%) sera. Twenty one had MIF results indicative...
BACKGROUND Babesia microti is a parasite that infects red blood cells (RBCs) in mammals. It transmitted to humans by tick bites, transfusion, organ transplantation, and congenital acquisition. Although the natural history seroprevalence donors have been well described, gaps knowledge relevant transfusion remain. STUDY DESIGN AND METHODS Mice were infected with dilutions of parasitized address minimal infectious dose kinetics parasitemia quantitative polymerase chain reaction (qPCR)...
The current Centers for Disease Control and Prevention (CDC) interpretive criteria serodiagnosis of Lyme disease (LD) involve a two-tiered approach, consisting first-tier EIA, IFA, or chemiluminescent assay, followed by confirmation positive equivocal results either immunoblot second-tier EIA. To increase overall sensitivity, single-tier alternative assays have been proposed, often utilizing antigens from multiple Borrelia burgdorferi strains genospecies in single immunoblot; including OspA...
Background Babesia, a tick-borne genus of intraerythrocytic parasites, is understudied in humans outside established high-endemic areas. There paucity data on Babesia Africa, despite evidence that it regionally present. A pilot study suggested was present rural district Tanzania. Methodology/Principal findings cross-sectional conducted July-August 2017: residents case hamlet had clustering subjects with high signal-to-cut off (S/CO) ratios for antibodies against B. microti the study, and...
ABSTRACT It has been proposed that Borrelia burgdorferi infection is present in ∼25% of children with autism spectrum disorders. In this study, antibodies against were assessed autistic ( n = 104), developmentally delayed 24), and healthy control 55) children. No seropositivity was detected the without autism. There no evidence an association between Lyme disease