A5100429127- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Biomedical Research and Pathophysiology
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Renal and related cancers
- Genetic Syndromes and Imprinting
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Trace Elements in Health
- Mitochondrial Function and Pathology
- Sulfur Compounds in Biology
- Bacterial biofilms and quorum sensing
- CRISPR and Genetic Engineering
- Aquaculture Nutrition and Growth
- Craniofacial Disorders and Treatments
- Eicosanoids and Hypertension Pharmacology
- Wheat and Barley Genetics and Pathology
- Cleft Lip and Palate Research
- Connective tissue disorders research
- MicroRNA in disease regulation
- Antimicrobial Resistance in Staphylococcus
- Fibroblast Growth Factor Research
- Circular RNAs in diseases
- Chemotherapy-induced organ toxicity mitigation
Botswana Geoscience Institute
2025
BGI Research
2025
South China Agricultural University
2024-2025
WinnMed
2020-2024
Mayo Clinic in Arizona
2021-2024
Mayo Clinic
2019-2024
Hebei University
2023
North Sichuan Medical University
2020-2021
Sun Yat-sen University
2020
Third Affiliated Hospital of Sun Yat-sen University
2020
Although large numbers of long noncoding RNAs (lncRNAs) expressed in the mammalian nervous system have been detected, their functions and mechanisms regulation remain to be fully clarified. It has reported that lncRNA antisense transcript for β‑secretase‑1 (BACE1‑AS) is elevated Alzheimer's disease (AD) drives rapid feed‑forward β‑secretase, suggesting it critical AD development. In present study, senile plaque (SP) SH‑SY5Y cell model was established using synthetic amyloid β‑protein (Aβ)...
Point mutations with unclear molecular mechanisms are often associated vancomycin resistance in Staphylococcus aureus. Here, we observed that the walK (G223D) mutation caused decreased expression of genes cell wall metabolism, autolytic activity, thickened walls, and reduced susceptibility. A phosphorylation assay showed WalK exhibited autophosphorylation, which led to WalR. An electrophoretic mobility shift indicated (G223D)-phosphorylated WalR had a capacity bind atlA promoter.
Wheat inflorescence architecture, particularly spikelet and floret development, is critical for grain yield. To decode the cellular transcriptional regulatory network (cTRN) underlying wheat we integrated multiple single-cell omics technologies to construct a spatiotemporal atlas of chromatin accessibility dynamics. This comprehensive analysis identified 20 cell types, 7,211 type-specific genes 152,333 accessible regions (csACRs) in inflorescence. Trajectory two sub-clusters proliferating...
<title>Abstract</title> In wheat, inflorescence architecture critically determines yield potential, yet its structural complexity and asynchronous development have hindered cellular-resolution studies of spikelet floret formation. Here, we integrate spatial transcriptomics, high-sensitivity multiplexed error-robust fluorescence <italic>in situ</italic> hybridization (MERFISH), snRNA-seq across six developmental stages to generate a spatiotemporal atlas the wheat inflorescence. We identified...
Preadipocytes dynamically produce sensory cilia. However, the role of primary cilia in preadipocyte differentiation and adipose homeostasis remains poorly understood. We previously identified transition fiber component FBF1 as an essential player controlling selective import. Here, we establish Fbf1tm1a/tm1a mice discover that develop severe obesity, but surprisingly, are not predisposed to adverse metabolic complications. Obese possess unexpectedly healthy white fat tissue characterized by...
Numerous studies have indicated that cells and tissues means of blocking their response to continuous stress signals protect themselves from damage. Overexpression angiotensin II (Ang II) in the renin-angiotensin system can cause vascular endothelial damage, but mechanism adjustment dynamic equilibrium remains unclear. In this study, we investigated whether microRNA-155 (miR-155) suppress Ang would otherwise We isolated cultured human umbilical vein (HUVECs) transfected one group these with...
Abstract Cellular senescence contributes to tissue homeostasis and age-related pathologies. However, how is initiated in stressed cells remains vague. Here, we discover that exposure irradiation, oxidative or inflammatory stressors induces transient biogenesis of primary cilia, which are then used by communicate with the promyelocytic leukemia nuclear bodies (PML-NBs) initiate responses human cells. Mechanistically, a ciliary ARL13B-ARL3 GTPase cascade negatively regulates association...
Abstract The canonical non-homologous end joining (c-NHEJ) repair pathway, generally viewed as stochastic, has recently been shown to produce predictable outcomes in CRISPR-Cas9 mutagenesis. This predictability, mainly 1-bp insertions and small deletions, led the development of in-silico prediction programs for various animal species. However, predictability CRISPR-induced mutation profiles across species remained elusive. Comparing between human plant reveals significant differences...
LncRNA FEZF1-AS1 has been reported to be an oncogene in many types of cancer, while its role glioblastoma (GBM) is unknown. This study aimed investigate the potential involvement GBM. expression paired GBM and non-tumor tissues from patients was determined by RT-qPCR. A 2-year follow-up performed analyze prognostic value for Cell transfections were interactions between FEZF1-AS1, miR-34a Notch-1. Transwell assay Notch-1 regulating cell invasion migration. In this study, analysis TCGA dataset...
Abstract Transition fibers (TFs) regulate cilia gating and make the primary cilium a distinct functional entity. However, molecular insights into biogenesis of gate remain elusive. In forward genetic screen in Caenorhabditis elegans , we uncover that TALP-3, homolog Joubert syndrome protein TALPID3, is TF-associated component. Genetic analysis reveals TALP-3 coordinates with ANKR-26, ANKRD26, to orchestrate proper gating. Mechanistically, ANKR-26 form complex key component DYF-19, FBF1....
Protein misfolding, aggregation, and accumulation cause neurodegenerative disorders. One such disorder, Huntington's disease, is caused by an increased number of glutamine-encoding trinucleotide repeats CAG in the first exon huntingtin (HTT) gene. Mutant proteins Htt 1 with polyglutamine expansion are prone to aggregation form pathological inclusion bodies neurons. Extensive studies have shown that misfolded cleared ubiquitin-proteasome system or autophagy alleviate their cytotoxicity....
<title>Abstract</title> The canonical non-homologous end joining (c-NHEJ) repair pathway has generally been considered a highly efficient and stochastic process. Yet recent studies indicated that c-NHEJ-mediated CRISPR-Cas9 mutagenesis, primarily leading to 1-bp insertions small deletions, may be predictable. This observation spurred the development of in-silico programs with remarkable accuracy in various animal species. Nevertheless, predictability CRISPR-induced mutation profiles across...
Abstract Serving as the cell's sensory antennae, primary cilia are linked to numerous human genetic diseases when they malfunction. DZIP1L, identified one of causes autosomal recessive polycystic kidney disease (ARPKD), is an evolutionarily conserved ciliary basal body protein. Although it has been reported that DZIP1L involved in entry PKD proteins, underlying mechanism remains elusive. Here, uncharacterized role modulating architecture and function transition fibers (TFs), striking base...
Despite the importance of cellular senescence in human health, how damaged cells undergo remains elusive. We have previously shown that promyelocytic leukemia nuclear body (PML-NBs) translocation ciliary FBF1 is essential for induction stressed cells. Here we discover an early event occurring transient assembly stress-induced nucleus-to-cilium microtubule arrays (sinc-MTs). The sinc-MTs are distinguished by unusual polyglutamylation and unique polarity, with minus-ends nucleating near...
Tubulin polyglutamylation is a posttranslational modification that occurs primarily along the axoneme of cilia. Defective impairs cilia function and has been correlated with ciliopathies, including Joubert Syndrome (JBTS). However, precise mechanisms regulating proper remain vague. Here, we show cyclin-dependent kinase 6 (CDK6), but not its paralog CDK4, localizes to base suppresses by phosphorylating RAB11 family interacting protein 5 (FIP5) at site S641, critical regulator import...
We have isolated a full-length cDNA clone (thymic stromal origin (TSO)-1C12) from SCID thymus library using probe PCR-based subtractive enriched for sequences fetal thymic cells. TSO-1C12 mRNA is expressed mainly in the cortex and highly thymus. Expression per cell highest during development decreases after day 16. Antipeptide Abs immunoprecipitated hydrophobic, plasma membrane glycoprotein cotransporter, TSCOT) whose translated sequence has weak homology to bacterial antiporters mammalian...
Staphylococcus aureus is an important Gram-positive pathogen responsible for numerous diseases ranging from localized skin infections to life-threatening systemic infections. The virulence of S. essentially determined by a wide spectrum factors, including cell wall-associated proteins and secreted toxins that are precisely controlled in response environmental changes. GGDEF domain protein (GdpS) the only conserved staphylococcal involved not c-di-GMP synthesis but regulation NCTC8325. Our...
HYLS1 regulates the ciliary phosphoinositide and hedgehog pathways, implicating a novel pathogenic mechanism for HLS syndrome.