A5000149039- Cardiac Ischemia and Reperfusion
- Nitric Oxide and Endothelin Effects
- Molecular Junctions and Nanostructures
- Quantum-Dot Cellular Automata
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Nanopore and Nanochannel Transport Studies
- Proteoglycans and glycosaminoglycans research
- Eicosanoids and Hypertension Pharmacology
- Nanocluster Synthesis and Applications
- RNA and protein synthesis mechanisms
- Cardiac Imaging and Diagnostics
- Pharmacological Receptor Mechanisms and Effects
- Surface Chemistry and Catalysis
- Animal testing and alternatives
- Advanced biosensing and bioanalysis techniques
- Neuropeptides and Animal Physiology
- Protein Degradation and Inhibitors
- Immune Cell Function and Interaction
- Protease and Inhibitor Mechanisms
- Neural dynamics and brain function
- Cardiac Arrest and Resuscitation
- Genetically Modified Organisms Research
- Photoreceptor and optogenetics research
Yale University
2025
University of Southampton
2024
Ingénierie Moléculaire et Physiopathologie Articulaire
2017-2021
Guangdong Ocean University
2020
International Islamic University, Islamabad
2020
University of Colorado Boulder
2017-2018
Energy Institute
2018
University of Colorado System
2018
Ohio University
2014
W.E. Upjohn Institute for Employment Research
1991-1998
A comparative analysis was carried out of the sensitivities in vitro vasorelaxations by nitroglycerine (NTG), acetylcholine (ACh) and nitric oxide (NO) to blockade glyburide, a blocker ATP-sensitive K+ channels (KATP), as well charybdotoxin (ChTX) iberiotoxin (lbTX), potent blockers calcium-activated (KCa). In isolated rabbit mesenteric artery (RMA) precontracted with 5 microM norepinephrine (NE), ACh (0.01-1 microM), NTG (0.01-5 microM) NO (0.075-2.7 produced dose-dependent vasodilation....
Synchronous neuronal activity is a hallmark of the developing mouse primary somatosensory cortex. While patterns synchronous in cortical layer 2/3 have been well described, source robust still unknown. Using novel microprism preparation and vivo 2-photon imaging neonatal mice, we show that organized barrel columns across layers. Monosynaptic rabies tracing slice electrophysiology experiments reveal pyramidal neurons receive significant 5 inputs during first postnatal week, silencing synaptic...
This study in isolated rabbit superior artery (RMA) investigated the interactions between glyburide, a known blocker of vascular ATP-sensitive K+ channels (KATP), and several chemically diverse potassium channel openers (PCOs): minoxidil sulfate (MNXS; 5 microM), pinacidil (1 cromakalim (0.5 microM) RP-49356 microM; PCO from Rhône Poulenc). Relaxation time courses for these PCOs were obtained norepinephrine (NE; microM)-precontracted RMA, concentrations found to be equipotent each other...
Abstract TMEM165 deficiency leads to skeletal disorder characterized by major dysplasia and pronounced dwarfism. However, the molecular mechanisms involved have not been fully understood. Here, we uncover that impairs synthesis of proteoglycans producing a blockage in elongation chondroitin-and heparan-sulfate glycosaminoglycan chains leading with shorter chains. We demonstrated is due defect Golgi elongating enzymes but rather availability co-factor Mn 2+ . Supplementation cell rescue...
This study investigated nitroglygerin (NTG) relaxations in isolated dog coronary artery comparison with other vascular preparations. Under maximal PNU-46619 precontraction, the was significantly more sensitive to NTG than mesenteric artery, vein and saphenous vein. In (1-100 nM) produced EC50 = 9.4 nM. KCl-contracted arteries (20-80 mM KCl), relaxation by progressively reduced. Relaxation responses also were inhibited potent calcium-activated K+ (BK) channel blockers, charybdotoxin (100...
This study describes the in vitro and vivo characteristics of a guanidine 4-morpholinecarboximidine-N-1-adamantyl-N'-cyclohexyl-hydroc hloride (U-37883A), as an antagonist vascular ATP-sensitive K+ channels (KATP). In isolated rabbit mesenteric artery, antagonistic effects U-37883A (0.5-5 microM) were studied against vasorelaxation produced by cromakalim (0.5 microM), minoxidil sulfate (5 pinacidil (1 RP-49356 microM). The dose-response curves for all four potassium channel openers similar...
The mechanisms of pinacidil-induced direct vasodilation were studied in vitro RMA and RAO. In RMA, pinacidil produced dose-dependent relaxations norepinephrine (5 microM)-induced contractions with an IC50 0.2 microM. This component relaxation appeared to be dependent on K+ conductance because pretreatment tetraethylammonium (10 mM), Ba++ (0.5 glyburide (1 microM) 20 mM all caused a rightward shift the dose-response curve (DRC) corresponding increase IC50. However, additional effects still...
Several nanoscale electronic methods have been proposed for high-throughput single-molecule nucleic acid sequence identification. While many studies display a large ensemble of measurements as "electronic fingerprints" with some promise distinguishing the DNA and RNA nucleobases (adenine, guanine, cytosine, thymine, uracil), important metrics such accuracy confidence base calling fall well below current genomic methods. Issues unreliable metal–molecule junction formation, variation...
Using a series of functional criteria, we wished to evaluate the K+ conductance mechanism and cyclic GMP implicated in actions nicorandil (NIC) as vasodilator. In rabbit isolated superior mesenteric artery, NIC exhibited two relaxation dose-response curves (DRCs): one with lower IC50 4.8 x 10(-6) M for norepinephrine (NE 5 microM) contraction, another higher 1.4 10(-4) 80 mM contraction. channel blockers (TEA 1-10 mM), Ba2+ (0.1-0.5 glyburide (1 microM), increased [K+]ex (20 all caused...
XPO1 (Exportin-1/CRM1) is a nuclear export protein that frequently overexpressed in cancer and functions as driver of oncogenesis. Currently small molecules target are being used the clinic anticancer agents. We identify for natural killer (NK) cells. Using immunopeptidomics, we have identified peptide derived from can be recognized by activating NK cell receptor KIR2DS2 context human leukocyte antigen–C. The endogenously processed presented to activate cells specifically through this...
We describe a nanoelectronic method for identifying single ribonucleotides and structural modifications, laying groundwork single-molecule RNA sequencing/structural mapping.
Glyburide, a sulfonylurea, and U-37883A, guanidine (4-Morpholinecarboximidine-N-1-Adamantyl-N' cyclohexylhydrochloride), have been previously characterized as antagonists of the vascular ATP-sensitive K+ channels (KATP). In this report, in vitro interaction between these two chemically distinct KATP was investigated using isolated rabbit mesenteric artery. The antagonism functionally studied inhibition vasodilation produced by various openers follows: pinacidil (1 microM), minoxidil sulfate...
Nanoelectronic DNA sequencing can provide an important alternative to sequencing‐by‐synthesis by reducing sample preparation time, cost, and complexity as a high‐throughput next‐generation technique with accurate single‐molecule identification. However, noise signature overlap continue prevent high‐resolution results. Probing the molecular orbitals of chemically distinct nucleobases offers path for facile sequence identification, but entropy (from nucleotide conformations) makes such...
KATP blockers derived from cyanoguanidine opener (P1075) chemistry were characterized in isolated rabbit mesenteric artery and evaluated functionally by their ability to antagonize maximal relaxation induced pinacidil (1 microM) of norepinephrine (5 contraction. PNU-89692, PNU-97025E PNU-99963 identified as with IC50 values 860, 83 18 nM, respectively. Studies selected chiral compounds demonstrated that the (R)-enantiomers more potent than (S)-enantiomers. Further studies (1) inhibited...
ATP-sensitive potassium (K+Atp) channel openers such as cromakalim and pinacidil exhibit both potent vasodilatory anti-ischemic properties. U-89232, a cyanoguanidine analog of cromakalim, has recently been found to myocardial protection during ischemia without altering in vivo hemodynamics. We examined the effects isolated vascular cardiac tissue tested whether glyburide, Katp blocker, could antagonize their effects. All three compounds produced concentration-dependent relaxation...