A5048396571- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Hormonal and reproductive studies
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Bee Products Chemical Analysis
- Salmonella and Campylobacter epidemiology
- Seed Germination and Physiology
- Milk Quality and Mastitis in Dairy Cows
- Male Reproductive Health Studies
- Veterinary Equine Medical Research
- Urologic and reproductive health conditions
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Heat shock proteins research
- Reproductive biology and impacts on aquatic species
- Urological Disorders and Treatments
- T-cell and Retrovirus Studies
- Ovarian function and disorders
- Healthcare and Venom Research
- Listeria monocytogenes in Food Safety
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Enterobacteriaceae and Cronobacter Research
- Endoplasmic Reticulum Stress and Disease
- Viral gastroenteritis research and epidemiology
Univates
2014-2024
Universidade Federal de Pelotas
2024
Bayer (Germany)
2020
Universidade Federal do Rio Grande do Sul
2005-2020
Hospital de Clínicas de Porto Alegre
2008-2020
Ludwig-Maximilians-Universität München
2020
Universidade Luterana do Brasil
2012
State University of Norte Fluminense
2009
The identification of biomarkers associated with seminal traits could aid in the selection higher quality ejaculates and benefit swine industry. objective this study was to identify boar plasma proteins sperm motility morphology. Twenty from fifteen adult boars a commercial stud were used for work. After routine semen collection analysis, classified into two groups: high-quality (HQS) low-quality (LQS), based on Semen samples processed separation analysis by 2D SDS-PAGE. Total progressive...
The epididymis is a complex organ where spermatozoa acquire motility and ability to fertilize the egg.Epididymal maturation lasts 1 or 2 weeks exposes immature sequentially modified milieu, promoting intense interactions with secretions by epididymal epithelium.Sperm surface modifications in response are key steps achieve fertility ability.However, precise molecular mechanisms that convert an immotile infertile gamete into highly motile cell capable of fusion oocyte still unknown.Recent data...
Abstract The present study was conducted to decipher the proteome of in vivo‐produced pre‐implantation ovine embryos. Ten locally adapted Morana Nova ewes received hormonal treatment and were inseminated 12 hr after ovulation. Six days later, 54 embryos (morula blastocyst developmental state) recovered from eight pooled obtain sufficient protein for proteomic analysis. Extracted embryo proteins analysed by LC‐MS/MS, followed identification based on four database searches (PEAKS, Proteome...
Although boar semen productivity is affected by seasonality, its effects are not equal among different regions which raise concerns regarding the profitability of stud farms. Therefore, goals this study were (i) to evaluate seasonal effect on production in a commercial farm located subtropical climate region and (ii) verify whether activities superoxide dismutase glutathione peroxidase spermatozoa seminal plasma associated with traits fresh cooled semen. Nine boars collected twice per...
Milkborne transmission of Shiga toxin- producing Escherichia coli (STEC) has raised considerable concern due to recent outbreaks worldwide and poses a threat public health. The aim this study was develop sensitive specific multiplex PCR assay detect the presence STEC in bovine raw milk. To identify E. (ATCC 25922) contamination, gene uspA used, sensitivity specificity were accessed by testing diluted samples ranging from 2 2.0 × 10(6) CFU/mL. STEC, stx1 stx2 genes selected as targets. After...
Hypogonadism is defined as the inadequate gonadal production of testosterone. Low serum testosterone leads to infertility by impairing spermatogenesis and reducing sperm count, however, impact hypogonadism in epididymal maturation poorly understood. From testis, spermatozoa are transported into epididymis where they find a specific microenvironment composed complex mixture proteins that facilitate storage maturation. Inside ductule, undergo several changes, resulting their becoming capable...
Abstract Background Hypothermic storage at 5°C has been investigated as an alternative to promote the prudent use of antibiotics for boar artificial insemination doses. However, this temperature is challenging some ejaculates or boars. Objective The present study aimed identify putative biomarkers semen resistance hypothermic by comparing seminal plasma proteomes boars with high and low preservation 5°C. Materials methods From initial group 34 boars, 15 were selected based on following...
Osteopontin (OPN) is a multifunctional phosphoprotein that has been linked to fertility in bulls. However, the exact mechanism by which OPN contributes fertilisation yet unknown. The biotechnological use of bovine reproduction promising but some gaps remain unfilled. present work aimed: (a) verify whether seminal plasma associated with traits and standard breeding soundness exam; (b) predict interactions integrins, CD44 glycosaminoglycans through molecular docking; (c) develop protocol for...
Sperm cells rely on different substrates to fulfil thei energy demand for functions and diverse moments of their life. Species specific mechanism involve both substrate transport utilization: hexose transporters, a protein family facilitative passive transporters glucose other hexose, have been identified in spermatozoa species and, within the species, localization has some cases, linked glycilitic enzyme presence. The catabolism sources purposes studied various recent advances made...
Despite the development of efficient boar semen extenders, there is still room for improvement new formulas using molecules that could increase fertilisation outcomes and substitute cryoprotectants antibiotics. The goal this work was to evaluate if essential oils from leaves Myrrhinium atropurpureum Cymbopogon citratus are suitable as additives in extender. major compounds found M. were 1,8-cineole (37.37%) terpinolene (19.18%); geranial (49.8%) neral (33.24%) oil C. citratus. addition 1%...
Endometrial cytology is a fast and reliable way to assess the uterus status breeding or insemination.Ultrasound examination may detect presence of uterine luminal fluid (IUF), which suggests susceptibility persistent endometritis.The aims this study were (1) test three different protocols for endometrial sampling (2) compare ultrasonographic evaluation diagnostic inflammation.Experiment 1-Twenty mares submitted one following cytology: (i) flush -60 ml PBS infused into via cuffed catheter.The...
Durante o processo de criopreservação sêmen, os espermatozóides sofrem alguns danos que resultam na diminuição da fertilidade deste. O presente estudo foi realizado com objetivo avaliar efeitos utilização combinada duas curvas congelamento dois diluentes comerciais (FR-5® e Botu-Crio®) sobre a sêmen eqüino. Foram analisados 20 ejaculados garanhões. As amostras foram avaliadas por microscopia contraste fase epifluorescência, observando-se motilidade progressiva total do pós-descongelamento...