A5026185844- Tuberculosis Research and Epidemiology
- Biochemical and Molecular Research
- Sperm and Testicular Function
- Mycobacterium research and diagnosis
- Hormonal and reproductive studies
- RNA and protein synthesis mechanisms
- vaccines and immunoinformatics approaches
- HIV/AIDS drug development and treatment
- Healthcare and Venom Research
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Research on Leishmaniasis Studies
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Adenosine and Purinergic Signaling
- Microbial Metabolism and Applications
- Iron Metabolism and Disorders
- Bee Products Chemical Analysis
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer therapeutics and mechanisms
- Protein purification and stability
- Insect Resistance and Genetics
- Blood properties and coagulation
- Bacteriophages and microbial interactions
- Entomopathogenic Microorganisms in Pest Control
Pontifícia Universidade Católica do Rio Grande do Sul
2017-2024
Sociedade Brasileira de Biologia Celular
2020
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2020
Genome annotation pipelines traditionally exclude open reading frames (ORFs) shorter than 100 codons to avoid false identifications. However, studies have been showing that these may encode functional microproteins with meaningful biological roles. We developed µProteInS, a proteogenomics pipeline combines genomics, transcriptomics and proteomics identify novel in bacteria. Our employs model filter out low confidence spectra, the need for manually inspecting Mass Spectrometry data. It also...
Abstract Background Hypothermic storage at 5°C has been investigated as an alternative to promote the prudent use of antibiotics for boar artificial insemination doses. However, this temperature is challenging some ejaculates or boars. Objective The present study aimed identify putative biomarkers semen resistance hypothermic by comparing seminal plasma proteomes boars with high and low preservation 5°C. Materials methods From initial group 34 boars, 15 were selected based on following...
ABSTRACT IQG-607 is a metal complex previously reported as promising anti-tuberculosis (TB) drug against isoniazid (INH)-resistant strains of Mycobacterium tuberculosis . Unexpectedly, we found that INH-resistant clinical isolates were resistant to IQG-607. Spontaneous mutants subjected whole-genome sequencing, and all sequenced colonies carried alterations in the katG gene. The (S315T) mutation was sufficient confer resistance both MIC assays inside macrophages. Moreover, overexpression...
Ureases catalyze the hydrolysis of urea into carbamate and ammonia. Well-conserved proteins, most plant ureases are hexamers a single chain subunit, like abundant isoform jack bean (Canavalia ensiformis) urease (JBU). Canatoxin (CNTX) was originally isolated from these seeds as neurotoxic protein, later characterized an JBU with lower molecular mass enzyme activity. Inactive CNTX oligomers form upon storage stabilization achieved by treatment low concentration formaldehyde, avoiding its...
Purine nucleoside phosphorylase from Mycobacterium tuberculosis (MtPNP), encoded by deoD gene (Rv3307), is an enzyme the purine salvage pathway, which has been widely studied as a molecular target for development of inhibitors with potential antimycobacterial activity. However, role MtPNP in pathogenesis and dormancy still unknown. The present work aims to construct knockout strain M. tuberculosis, evaluate growth under oxygenated condition model, assess whether important invasion...
Functional and structural data suggested that His<sup>197</sup>and Ser<sup>322</sup>residues play a role in<italic>Lb</italic>ASL catalysis.
Abstract Tuberculosis (TB) is an infectious disease caused mainly by Mycobacterium tuberculosis (Mtb) and responsible for millions of deaths. New Mtb strains resistant to TB drugs are emerging spreading. The first-line drug, isoniazid (INH), must be activated inside mycobacterial cells the catalase-peroxidase enzyme KatG exert its antimicrobial activity, mutations on katG gene a significant cause INH resistance in clinics. metal-containing compound IQG-607 analog developed inhibit target...
Tuberculosis remains a burden to this day, due the rise of multi and extensively drug-resistant bacterial strains. The genome Mycobacterium tuberculosis (Mtb) strain H37Rv underwent an annotation process that excluded small Open Reading Frames (smORFs), which encode class peptides proteins collectively known as microproteins. As result, there is overlooked part its proteome rich source potentially essential, druggable molecular targets. Here, we employed our recently developed proteogenomics...
We found that cells from Mycobacterium smegmatis , a model organism safer and easier to study than the disease-causing mycobacterial species, when depleted of an enzyme shikimate pathway, are auxotrophic for three aromatic amino acids (AroAAs) serve as building blocks cellular proteins: l- tryptophan, l -phenylalanine, -tyrosine. That supplementation with only AroAAs is sufficient rescue viable pathway inactivated was unexpected, since this produces end product, chorismate, starting compound...
Inosine–uridine nucleoside hydrolase from<italic>Leishmania braziliensis</italic>is a nonspecific enzyme that contains disulfide bond not needed for tetramer stabilization.
ABSTRACT Tuberculosis remains a burden to this day, due the rise of multi and extensively drug-resistant bacterial strains. The genome Mycobacterium tuberculosis (Mtb) underwent an annotation process that excluded small Open Reading Frames (smORFs), which encode class peptides proteins collectively known as microproteins. As result, there is overlooked part its proteome rich source potentially essential, druggable molecular targets. Here, we employed our recently developed proteogenomics...
Abstract The epidemiological importance of bacteria from the genus Mycobacterium is indisputable and necessity to find new molecules that can inhibit their growth urgent. shikimate pathway, required for synthesis important metabolites in bacteria, represents a target inhibitors tuberculosis growth. aroA -encoded 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme catalyzes sixth step pathway. In this study, we combined gene knockout, knockdown kinetic assays evaluate essentiality...