The three-dimensional structure of a protein plays an important role in dynamics the biological system human. By now, it remains challenge to characterize and quantify shape at single-molecule level. nanopores, as novel sensor, has been widely applied many fields such DNA sequencing human diseases diagnosis. In this paper, we investigated translocation spherelike con.A prolate bovine serum albumin (BSA) under electric field by solid-state nanopore. analyzing ionic current, BSA could be...

Ion transport through nanopores is a process of fundamental significance in nature and engineering practice. Over the past decade, it has been found that ion conductivity could be drastically enhanced, different mechanisms have proposed to explain this observation. To date, most reported studies carried out with relatively dilute electrolytes, while under high electrolyte concentrations (>1 M) rarely explored. Through systematic experimental atomistic simulation NaCl solutions, here we show...

Monolayer and/or atomically thin transition metal dichalcogenides cover a wide range of two-dimensional (2D) materials, whose fascinating semiconducting and optical properties have made them promising candidate materials for optoelectronic devices. Controllable growth these is critical their device applications. By using MoCl5 H2S as precursors, monolayer ultrathin molybdenum disulfide (MoS2) films with controlled lamellar structure been directly built layer by on SiO2 substrates without...

Releasing fluids from nanochannels is quite challenging, yet crucial for the application of nanofluidic systems, <italic>e.g.</italic> drug delivery and nanoprinting.

Solid state nanopores have emerged as an important electrical label-free single-molecule detection platform. While much effort has been spent on analyzing the current trace to determine size, shape and charge of translocating species, a less studied aspect is number events how this relates analyte concentration. In work we systematically investigate event frequency depends voltage applied across pore show that dependence can be utilized target Importantly, method does not require any...

A challenge that remains to be solved in the high-throughput and low-cost nanopore DNA sequencing is translocates through too quickly sequenced with enough accuracy.

Ion transport through nanopores is an important process in nature and has engineering applications. To date, most studies of nanopore ion have been carried out with electrolytes relatively low concentrations. In this paper, we report on ionic current modulation from the translocation dsDNA a under high strength electrolyte concentration gradient across nanopore. Results show that case, DNA can induce either negative or positive modulation, even though usually only downward peaks are expected...

The function of a protein is determined by the composition amino acids and essential to proteomics. However, sequencing remains challenging due protein's irregular charge state its high-order structure. Here, proof principle study on capability graphene nanopores integrated with atomic force microscopy performed using molecular dynamics simulations. It found that can discriminate sequence even protonation at single-residue resolution. Both pulling forces current blockades induced permeation...

Extensive molecular dynamics simulations are employed to optimize nanopore size and surface charge density in order obtain high ionic selectivity water throughput for seawater desalination systems. It is demonstrated that with the help of exclusion, nanopores diameter as large 3.5 nm still have selectivity. The mechanism salt rejection a surface-charged mainly attributed ion concentration difference between cations anions induced by charges. Increasing beneficial enhance However, there...

Abstract Solid‐state nanopores can be a powerful tool to investigate proteins in their native state. However, the excessive fast translocation speed of causes majority events remain undetected. Here, behaviors streptavidin (SA) transport through solid‐state nanopore are dominated by electroosmotic flow (EOF). Experimental results show frequency detected with diameter slightly larger than length is ≈17 times that previously reported work. Numerical simulations elucidate enhanced comes from...

Molecular machines hold keys to performing intrinsic functions in living cells so that the organisms can work properly, and unveiling mechanism of functional molecule as well elucidating dynamic process interaction with their surrounding environment is an attractive pharmaceutical target for human health. Due limitations searching exploring all possible motors bodies, designing constructing nanorobots vital meeting fast-rising demand revealing life science related diagnostics. Here, we...

Surface charges inside a nanopore determine the zeta potential and ion distributions play significant role in affecting transport sensitivity of detecting biomolecules. It is great importance to study fluctuation surface with salt concentration pH various applications nanopores. Herein, we proposed theoretical model predict charge density Si3N4 nanopore, which both silanol amine groups were taken into account. was demonstrated that changes not only but also concentration. The could well...

Abstract During the past decades, scientists have developed different kinds of nanorobots based on various driving principles to realize controlled manipulation them for potential applications like medical diagnosis and directed cargo delivery. In order design a nanorobot with advantages simple operation precise control that can enrich family intelligent nanorobots, an encoding method is proposed movement DNA‐nanoparticle assembled by combing electrophoresis electroosmosis effect in...

Nanopore analysis relies on ensemble averaging of translocation signals obtained from numerous molecules, requiring a relatively high sample concentration and long turnaround time the to results. The recapture subsequent re-reading same molecule is promising alternative that enriches signal information single molecule. Here, we describe how an asymmetric nanopore improves molecular ping-pong by promoting in trans reservoir. We also demonstrate could be improved linking target DNA carrier...

The detection and identification of nanoscale molecules are crucial, but traditional technology comes with a high cost requires skilled operators. Solid-state nanopores new powerful tools for discerning the three-dimensional shape size molecules, enabling translation molecular structural information into electric signals. Here, DNA different shapes were designed to explore effects electroosmotic forces (EOF), electrophoretic (EPF), volume exclusion on signals within solid-state nanopores....

In the past few decades, nanometer-scale pores have been employed as powerful tools for sensing biological molecules. Owing to its unique structure and properties, solid-state nanopores provide interesting opportunities development of DNA sequencing technology. Controlling translocation in is an important means improving accuracy sequencing. Here we present a proof principle study accelerating captured across targeted graphene using surface charge density find intrinsic mechanism combination...

Nanobubbles play an important role in diverse fields, including engineering, medicine, and agriculture. Understanding the characteristics of individual nanobubbles is essential for comprehending fluid dynamics behaviors advancing nanoscale science across various fields. Here, we report a strategy based on nanopore sensors characterizing single-digit nanobubbles. We investigated sizes diffusion coefficients at different voltages. Additionally, finite element simulation molecular were...

Abstract High translocation speed of a DNA strand through nanopore is major bottleneck fornanopore detection molecules. Here, we choose MgCl 2 electrolyte assalt solution to control mobility. Experimental results demonstrate that theduration time for straight state events in 1 M about 1.3 ms which three times longer than thesame KCl solution. This because Mg 2+ ions caneffectively reduce the surface charge density negative strands and thenlead decrease electrophoretic speed. It also found...

We use a resistive-pulse technique to analyze molecular hybrids of single-wall carbon nanotubes (SWNTs) wrapped in either single-stranded DNA or protein. Electric fields confined glass capillary nanopore allow us probe the physical size and surface properties at single-molecule level. find that translocation duration macromolecular hybrid is determined by its hydrodynamic solution mobility. The event current reveals effects ion exclusion rod-shaped possible due temporary polarization SWNT...

As the single molecule detection tool, solid-state nanopores are being applied in more and fields, such as medicine controlled delivery, ion conductance microscopes, nanosensors, DNA sequencing. The critical information obtained from is signal collected, which ionic block current caused by molecules passing through pores. However, collected is, part, impeded relatively low signal-to-noise ratio of nanopore measurements. Here, we report that using a salt gradient across could improve when...

Here we proposed a new strategy combining the nanopore series circuit and current clamp to get voltage trace across nanopore, hence producing blockade signal when DNA translocate. This exhibits an intriguing charging discharging phenomenon.

This work proposes a gold nanoparticle (AuNP) based probe to study the single-nanoparticle translocation behavior through solid-state nanopore using plasmon resonance energy transfer (PRET) effect.