A new tool for the high-throughput study of carbohydrate–protein interactions: Maleimide-linked carbohydrates (e.g. 1) were immobilized on thiol-derivatized glass slides, and microspots probed with fluorescein-labeled lectins (see picture). The binding to slide depends concentration length tethers.

Carbohydrate−protein interactions play important biological roles in living organisms. For the most part, biophysical and biochemical methods have been used for studying these biomolecular interactions. Less attention has given to development of high-throughput elucidate recognition events between carbohydrates proteins. In current effort develop a novel tool monitoring carbohydrate−protein interactions, we prepared carbohydrate microarrays by immobilizing maleimide-linked on...

Abstract The biological significance of glycans in the post‐genomic era requires development new technologies to enable functional studies carbohydrates a high‐throughput manner. Recently, carbohydrate microarrays have been exploited as an advanced technology for this purpose. Efficient immobilization methods probes on proper surface are essential successful fabrication microarrays. Up date, several techniques developed attach simple or complex solid surface. glycan applied glycomics, drug...

Carbohydrate microarrays have been used recently for the rapid analysis of glycan−protein or glycan−cell interactions and detection pathogens. As a demonstration its significance versatility, microarray technology has applied in this effort to assay glycosyltransferase activities. In addition, carbohydrate based methods employed quantitatively determine binding affinities between lectins carbohydrates.

In this review, we summarize the recent efforts on manipulating phonon transport in solids by using specific techniques that modify their thermal conductivity (i.e., heat, group velocity, and mean free path) conductance transmission probability density of states). The strategies discussed for tuning are as follows: large unit cell approach liquid-like conduction maneuvering heat; rattler, mini-bandgap, confinement velocity; nanoparticles, nanosized grains, coated alloy (isotope) scattering,...

A cell-based screening approach in which annexin V was used as a staining agent identified apoptozole (Az; see scheme) an imidazole-based molecule induces apoptosis. Az binds to Hsc70 and Hsp70 (anti-apoptotic chaperone proteins), with dissociation constants of 0.21 0.14 μM, respectively, thereby inhibiting their function. Thus, possesses antitumor activity is, therefore, potential cancer therapeutic agent. Supporting information for this article is available on the WWW under...

Carbohydrate microarrays have received great attention as high-throughput analytic tools in studies of carbohydrate-mediated biological processes. Most the methods employed to fabricate glycan rely on immobilization modified glycans properly derivatized surfaces. This strategy requires availability whose syntheses many cases are time-consuming and difficult. We developed a simple direct technique that involves one-step, site-specific attachment diverse unmodified hydrazide-derivatized glass...

Dual-modal fluorescent magnetic glyconanoparticles have been prepared and shown to be powerful in probing lectins displayed on pathogenic mammalian cell surfaces. Blood group H1- Leb-conjugated nanoparticles were found bind BabA displaying Helicobacter pylori, Lea- Leb-modified are both recognized by internalized into DC-SIGN SIGN-R1 expressing cells via lectin-mediated endocytosis. In addition, block adhesion of H. pylori cells, suggesting that they can serve as inhibitors infection host...

We investigated the relationship between defects in graphene and NO2 gas sensitivity of graphene-based sensors. Defects were introduced by hydrogen plasma or ultraviolet (UV)/ozone treatment. As defect concentration increased, was enhanced, sub-ppb level detection limit achieved. UV irradiation used for recovery at room temperature. However, generated ozone treatment, like oxide, reduced back to irradiation, so ozone-treated sensor not stable over time. In contrast, response hydrogenated...

We fabricated devices consisting of single and double graphene sheets embedded in organic polymer layers. These had binary ternary nonvolatile resistive switching behaviors, respectively. Capacitance–voltage (C–V) curves scanning capacitance microscopy (SCM) images were obtained to investigate the mechanism. The C–V exhibited a large hysteresis, implying that acted as charging discharging layers was caused by charges trapped In addition, capacitive behaviors observed for device with sheet,...

The thermal conduction characteristics of GeTe and Ge2Sb2Te5(GST) nanowires were investigated using an optical method to determine the local temperature by Raman spectroscopy. Since localization surface charge in a single-crystalline nanostructure can enhance charge-phonon scattering, conductivity value (κ) single crystalline GST was decreased significantly 1.44 Wm–1 K–1 for 1.13 GST, compared reported values polycrystalline structures. SET-to-RESET state are characteristic memory device....

We developed Schottky junction photovoltaic cells based on multilayer Mo1–xWxSe2 with x = 0, 0.5, and 1. To generate built-in potentials, Pd Al were used as the source drain electrodes in a lateral structure, graphene bottom top vertical structure. These devices exhibited gate-tunable diode-like current rectification responses. Mo0.5W0.5Se2 diodes higher efficiency than MoSe2 WSe2 electrodes, likely because of greater adjusted band alignment devices. Furthermore, we showed that...

The phase transition characteristics and device performance of Si-doped Ge2Sb2Te5 thin films were studied for PRAM application. deposited by the RF-magnetron cosputtering system analyzed x-ray diffractometer (XRD), 4-point probe measurement photoelectron spectroscopy (XPS). difference in sheet resistance between amorphous crystalline states was higher than 104 Ω/□. In XPS spectrum, Si–Te binding confirmed. addition, positional change Si observed. activation energy transformation decreased...

Multicomponent fluorescent hexapod coordination polymer particles (CPPs) have been synthesized from Zn2+ ions and two kinds of building blocks (1,4-biphenyldicarboxylic acid, H2bpdc N,N′-phenylenebis(salicyclideneimine)dicarboxylic 1). The ultimate size the final CPPs depended upon initially-formed cube CPPs, which acted as template for growth secondary polymers. SEM studies conducted at numerous stages revealed several interesting intermediates, give exceptionally strong evidence...

Microarray technologies have received considerable attention owing to the fact that they serve as powerful tools for high-throughput analysis of biomolecular interactions and identification bioactive substances bind biomolecules. Most current methods used construct microarrays rely on immobilization properly derivatized surfaces. Among various functional groups this purpose, N-hydroxysuccinimide (NHS) ester group has been largely employed because it can be readily reacted with amine or...

Ein neues Hilfsmittel für Hochdurchsatz-Untersuchungen von Kohlenhydrat-Protein-Wechselwirkungen: Maleinimid-verknüpfte Kohlenhydrate wie 1 werden an Thiol-derivatisierten Glasträgern immobilisiert und die Mikrospots anschließend mit Fluorescein-markierten Lectinen untersucht (siehe Bild). Die Bindung der Lectine auf dem Träger hängt Konzentration immobilisierten Länge des Spacers ab.

This cross-sectional study investigated the association between long working hours and prevalence of poor self-rated health (SRH) in Korean young workers using data from 6th Korea Youth Panel Survey 2007. A total 3317 wage were included. Weekly divided into four groups (36 to 40 hours, 41 52 53 60 more than per week). Multiple logistic regression was performed investigate SRH, adjusting for demographic work-related factors. Long associated with increased risk both men women. Legal...

Glycan microarrays have become powerful tools in the investigation of biological systems because they enable fast, quantitative, and simultaneous analysis glycan−protein interactions with small quantities samples. One hurdle construction glycan is to obtain a diverse set glycans for immobilization on surfaces. An article this issue ACS Chemical Biology describes microscale preparation fluorescently labeled reactive cyclic form reducing sugar, from natural sources, that can be utilized...