A5060621887- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- 3D Printing in Biomedical Research
- Skin and Cellular Biology Research
- Bone Tissue Engineering Materials
- Cell Image Analysis Techniques
- Neurobiology and Insect Physiology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- dental development and anomalies
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Immune cells in cancer
- RNA regulation and disease
- HIV Research and Treatment
- Planarian Biology and Electrostimulation
- Prion Diseases and Protein Misfolding
- Polymer Surface Interaction Studies
- T-cell and B-cell Immunology
- Alzheimer's disease research and treatments
- Amyotrophic Lateral Sclerosis Research
- Trace Elements in Health
- CRISPR and Genetic Engineering
German Center for Neurodegenerative Diseases
2015-2022
Forschungszentrum Jülich
2008-2012
Max Planck Institute for Neurobiology of Behavior – caesar
2005
Center of Advanced European Studies and Research
2005
A repeat expansion in the non-coding region of C9orf72 gene is most common mutation causing frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). Sense antisense transcripts are translated into aggregating dipeptide (DPR) proteins all reading frames (poly-GA,-GP,-GR,-PA -PR) through an unconventional mechanism. How these changes contribute to cytoplasmic mislocalization aggregation TDP-43 thereby ultimately leading neuron loss remains unclear. The RNA itself...
The coordinated formation and release of focal adhesions is necessary for cell attachment migration. According to current models, these processes are caused by temporal variations in protein composition. Protein incorporation into believed be controlled phosphorylation. Here, we tested the exchange dynamics GFP-vinculin as marker using method Fluorescence Recovery After Photobleaching. relevance phosphorylation state protein, age acting force were investigated. For stable stationary...
The spatially ordered formation and disassembly of focal adhesions is a basic requirement for effective cell locomotion. Because couple the contractile actin–myosin network to substrate, their distribution determines pattern traction forces propelling in certain direction. In present study, we quantitatively analyzed spatial patterning cell–substrate adhesion migrating cells by mapping averaged growth dynamics standardized coordinate system. These maps revealed distinct zones assembly,...
Migration of cells is one the most essential prerequisites to form higher organisms and depends on a strongly coordinated sequence processes. Early migratory events include substrate sensing, adhesion formation, actin bundle assembly force generation. While sensing was ascribed filopodia, all other processes were believed depend mainly lamellipodia migrating cells. In this work we show for motile keratinocytes that from generation filopodial focal complexes as well bundles. step by process...
NK cells, a heterogeneous sub-population of lymphocytes, are critically involved in the regulation both innate and adaptive immune responses humans. Besides their participation control tumors viral infections, they also regulate inflammatory processes, mediating beneficial detrimental effects. To effectively fulfil role surveillance, proper trafficking cells is essential. However, mechanisms factors governing cell recruitment only poorly dissected. Here, we describe functional tetraspanins,...
ABSTRACT Mammalian prions are unconventional infectious agents composed primarily of the misfolded aggregated host prion protein PrP, termed PrP Sc . Prions propagate by recruitment and conformational conversion cellular into abnormal aggregates on cell surface or along endocytic pathway. Cellular glycosaminoglycans have been implicated as first attachment sites for cofactors replication. Glycosaminoglycan mimetics obstruction glycosaminoglycan sulfation affect replication, but inhibitory...
The nervous system has the remarkable ability to adapt and respond various stimuli. This neural adjustment is largely achieved through plasticity at synaptic level. Active Zone (AZ) region presynaptic membrane that mediates neurotransmitter release composed of a dense collection scaffold proteins. AZs Drosophila melanogaster (Drosophila) photoreceptors undergo molecular remodeling after prolonged exposure natural ambient light. Thus level neuronal activity can rearrange composition AZ...
Human immunodeficiency virus (HIV) infection is associated with impaired natural killer (NK) cell activity, which only incompletely restored under antiretroviral therapy. Analyzing the bioenergetics profiles of oxygen consumption, we observed that several parameters were significantly reduced in HIV+ NK cells, indicating a mitochondrial defect. Accordingly, found CD56bright cells to display decreased membrane potential and mass. Both positively correlated interferon gamma (IFN-γ) production...
Journal Article Cryo-electron Tomography Workflows for Quantitative Analysis of Actin Networks Involved in Cell Migration Get access Florian Fäßler, Fäßler Institute Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria Search other works by this author on: Oxford Academic Google Scholar Georgi Dimchev, Dimchev Victor-Valentin Hodirnau, Hodirnau Bettina Zens, Zens Christoph Möhl, Möhl German Center Neurodegenerative Diseases (DZNE), Bonn, Nordrhein-Westfalen, Germany...
Traditionally, studies on protein translation rely systems, in which cells have been lysed prior determination of levels the interest. However, these assays do not reflect synthesis living real time, but analyze after a given incubation leading to limitations results based experimental parameters. To overcome this problem, we previously established Fluorescence recovery photobleaching (FRAP)-based technique monitor cells. For this, interest fused green fluorescent (GFP) is expressed cell...
Observations of single epidermal cells on flat adhesive substrates have revealed two distinct morphological and functional states, namely a non-migrating symmetric unpolarized state migrating asymmetric polarized state. These states are characterized by different spatial distributions dynamics important biochemical cell components: F-actin myosin-II form the contractile part cytoskeleton, integrin receptors in plasma membrane connect filaments to substratum. In this way, focal adhesion...
The nervous system has the remarkable ability to adapt and respond various stimuli. This neural adjustment is largely achieved through plasticity at synaptic level. Active Zone (AZ) region presynaptic membrane that mediates neurotransmitter release composed of a dense collection scaffold proteins. AZs Drosophila melanogaster (Drosophila) photoreceptors undergo molecular remodeling after prolonged exposure natural ambient light. Thus level neuronal activity can rearrange composition AZ...