A5031189107- Glycosylation and Glycoproteins Research
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Metabolism and Genetic Disorders
- Monoclonal and Polyclonal Antibodies Research
- Estrogen and related hormone effects
- Wnt/β-catenin signaling in development and cancer
- Nanoplatforms for cancer theranostics
- Amino Acid Enzymes and Metabolism
- Carbohydrate Chemistry and Synthesis
- Proteoglycans and glycosaminoglycans research
- Ubiquitin and proteasome pathways
- Genomics and Phylogenetic Studies
- Ion channel regulation and function
- Advanced Glycation End Products research
- ATP Synthase and ATPases Research
- Enzyme Structure and Function
- Computational Drug Discovery Methods
- Immune Response and Inflammation
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Adenosine and Purinergic Signaling
- Retinoids in leukemia and cellular processes
- Neuroscience and Neuropharmacology Research
- Hippo pathway signaling and YAP/TAZ
Chung-Ang University
2022-2025
Dongduk Women's University
2025
Duke University
2015-2022
Duke Medical Center
2006-2018
Duke University Hospital
2018
Yonsei University
2006-2016
Hallym University
2005
Hallym University Medical Center
2005
Kangdong Sacred Heart Hospital
2005
Mercy Medical Center
2004
Calcium transport plays an important role in regulating mitochondrial physiology and pathophysiology. The calcium uniporter (MCU) is a calcium-selective ion channel that the primary mediator for uptake into matrix. Here, we present cryo-electron microscopy structure of full-length MCU from Neurospora crassa to overall resolution ~3.7 angstroms. Our reveals tetrameric architecture, with soluble transmembrane domains adopting different symmetric arrangements within channel. conserved...
Abstract Lipopolysaccharide (LPS) triggers deleterious systemic inflammatory responses when released into the circulation. LPS‐binding protein (LBP) in serum plays an important role modifying LPS toxicity by facilitating its interaction with signaling receptors, which are expressed on surface of LPS‐responsive cells. We have previously demonstrated that high mobility group box 1 (HMGB1) can bind to and transfer LPS, consequently increasing LPS‐induced TNF‐α production human peripheral blood...
Parkinson's disease (PD) is a common neurodegenerative that involves the deterioration of dopaminergic neurons in substantia nigra pars compacta. Although etiology PD remains poorly understood, recent genetic, postmortem, and experimental evidence shows abnormal protein accumulation subsequent aggregate formation are prominent features both sporadic familial PD. While proteasome dysfunction observed PD, diverse mutations parkin gene linked to early-onset autosomal-recessive forms We...
<title>Abstract</title> Atherosclerosis is driven by chronic lipid accumulation, oxidative stress, and impaired autophagy, yet effective therapies targeting these pathways remain elusive. Oxidized low-density lipoprotein (oxLDL), a major contributor to atherosclerosis, disrupts cellular homeostasis promoting the formation of mitochondria-associated ER membrane (MAM), which regulate essential processes such as calcium transport, metabolism, mitochondrial dynamics, autophagy—critical...
The spiro compound, 5,5′-dimethoxy-1,3-bis(3-(trifluoromethyl)phenyl)-3,3a-dihydro-1H-spiro[cyclopenta[a]indene-2,2′-indene]-1′,8(3′H,8aH)-dione (4), was synthesized and identified by NMR spectroscopy, mass spectrometry, X-ray crystallography. Compound 4, C36H26F6O4, crystallized in the triclinic space group P-1with cell parameters a = 8.8669(5) Å, b 10.5298(8) c 17.0135(11) α 91.396(2)°, β 90.490(2)°, γ 109.235°, V 1499.14(17) Å3, Z 2. In an asymmetric unit, two molecules are packed short...
The EGFR-targeted monoclonal antibodies are a valid therapeutic strategy for patients with metastatic colorectal cancer (mCRC). However, only small subset of mCRC has benefits and there high demands EGFR therapeutics broader patient pool more potent efficacy. In this study, we report GC1118 exhibiting different character in terms binding epitope, affinity, mode action, efficacy from other anti-EGFR antibodies. Structural analysis the EGFR-GC1118 crystal complex revealed that recognizes...
Compared to other organs, the brain has limited antioxidant defenses. In particular, hippocampus is central region for learning and memory highly susceptible oxidative stress. Glial cells are most abundant in brain, sustained glial cell activation critical neuroinflammation that aggravates neuropathology neurotoxicity. Therefore, regulating a promising neurotherapeutic treatment. Quinic acid its derivatives possess anti-oxidant anti-inflammatory properties. Although previous studies have...
The PDZ domain-containing scaffold protein, syntenin-1, binds to the transmembrane proteoglycan, syndecan-4, but molecular mechanism/function of this interaction are unknown. Crystal structure analysis syntenin-1/syndecan-4 cytoplasmic domains revealed that syntenin-1 forms a symmetrical pair dimers anchored by syndecan-4 dimer. domain is compact intertwined dimer with clamp shape and two antiparallel strands forming cavity within dimeric twist. PDZ2 direct domain, inhibiting functions such...
PM0188 is a newly identified sialyltransferase from P. multocida which transfers sialic acid cytidine 5'-monophosphonuraminic (CMP-NeuAc) to an acceptor sugar. Although sialyltransferases are involved in important biological functions like cell-cell recognition, cell differentiation and receptor-ligand interactions, little known about their catalytic mechanism. Here, we report the X-ray crystal structures of presence sugar donor analogue, revealing precise mechanism transfer. Site-directed...
The interaction between the orphan nuclear receptor FTZ-F1 (Fushi tarazu factor 1) and segmentation gene protein FTZ is critical for specifying alternate parasegments in Drosophila embryo. Here, we have determined structure of ligand-binding domain (LBD)·FTZ peptide complex using x-ray crystallography. Strikingly, pocket LBD completely occupied by helix 6 (H6) receptor, whereas cofactor binds co-activator cleft site LBD. Our findings suggest that H6 essential transcriptional activity FTZ-F1;...
We developed a process to produce novel interactions between two previously unrelated proteins. This selects protein scaffolds and designs interfaces that bind surface patch of interest on target protein. Scaffolds with shapes complementary the were screened using an exhaustive computational search human proteome optimized by directed evolution phage display. method was applied successfully design epidermal growth factor receptor (EGFR) domain II, interface EGFR dimerization, high reactivity...
Murine protein serine/threonine kinase 38 (MPK38) is the murine orthologue of human maternal embryonic leucine-zipper (MELK), which belongs to SNF1/AMPK family. MELK considered be a promising drug target for anticancer therapy because overexpression and hyperactivation correlated with several cancers. Activation MPK38 requires extended sequence (ExS) containing ubiquitin-associated (UBA) linker UBA domain phosphorylation activation loop. However, mechanism unknown. This paper reports crystal...
Tyler, D.; Yoshimoto, Y.; Grubbs, E.; Yoo, J.; Zipfel, P.; Selim, A.; Augustine, C. Author Information