A5027618527- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiovascular Disease and Adiposity
- Cell Adhesion Molecules Research
- Apelin-related biomedical research
- Immune cells in cancer
- Hormonal Regulation and Hypertension
- Immune Response and Inflammation
- Renin-Angiotensin System Studies
- Antioxidant Activity and Oxidative Stress
- Inflammasome and immune disorders
- Adipose Tissue and Metabolism
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Eosinophilic Esophagitis
- Nuclear Receptors and Signaling
- Regulation of Appetite and Obesity
- Circular RNAs in diseases
- Atherosclerosis and Cardiovascular Diseases
- Eicosanoids and Hypertension Pharmacology
- Adipokines, Inflammation, and Metabolic Diseases
- Angiogenesis and VEGF in Cancer
- Ferroptosis and cancer prognosis
- Whipple's Disease and Interleukins
- Free Radicals and Antioxidants
Northwestern University
2021-2025
Northwestern University
2023
Nanjing Medical University
2016-2019
Zero to Three
2019
Center for Translational Molecular Medicine
2018
Inflammation is involved in pathogenesis of hypertension. NLRP3 inflammasome activation a powerful mediator inflammatory response via caspase-1 activation. The present study was designed to determine the roles and mechanisms phenotypic modulation proliferation vascular smooth muscle cells (VSMCs) Experiments were conducted spontaneously hypertensive rats (SHR) primary aortic VSMCs. observed media aorta SHR VSMCs from SHR. Knockdown inhibited activation, VSMC transformation SHR-derived...
Neutrophil (PMN) tissue accumulation is an established feature of ulcerative colitis (UC) lesions and colorectal cancer (CRC). To assess the PMN phenotypic functional diversification during inflammatory ulceration to CRC transition we analyzed transcriptomic landscape blood PMNs. Transcriptional programs effectively separated PMNs based on their localization peripheral blood, inflamed colon, tumors. In silico pathway overrepresentation analysis, protein-network mapping, gene signature...
Fibronectin type III domain-containing 5 (FNDC5) protein induces browning of subcutaneous fat and mediates the beneficial effects exercise on metabolism. However, whether FNDC5 is associated with hepatic steatosis, autophagy, fatty acid oxidation (FAO), lipogenesis remains unknown. Herein, we show roles mechanisms in lipid Fasted FNDC5−/− mice exhibited severe reduced FAO, enhanced liver compared wild-type mice. Energy deprivation–induced AMPK activity were attenuated hepatocytes, which...
The proliferation, migration and inflammation of vascular smooth muscle cells (VSMCs) contribute to the pathogenesis progression several cardiovascular diseases such as atherosclerosis hypertension. Angiotensin (Ang)-(1-7) Ang II are identified be involved in regulating activity. present study is designed determine interaction between Ang-(1-7) on VSMCs well their underlying mechanisms. We found that significantly suppressed positive effects inflammation, induction phosphorylation Akt ERK1/2...
Angiotensin (Ang) II plays vital roles in vascular inflammation and remodeling hypertension. Phenotypic transformation of smooth muscle cells (VSMCs) is a major initiating factor for remodeling. The present study was designed to determine the NLRP3 inflammasome activation Ang II-induced VSMC phenotypic hypertension.Primary VSMCs from aorta knockout (NLRP3-/-) mice wild-type (WT) were treated with 24 h. Subcutaneous infusion via osmotic minipump 2 weeks used induce hypertension WT NLRP3-/-...
Proliferation of vascular smooth muscle cells (VSMCs) plays crucial roles in remodelling and stiffening hypertension. Vascular adventitial fibroblasts are a key regulator wall function structure. This study is designed to investigate the fibroblasts-derived extracellular vesicles (EVs) VSMC proliferation normotensive Wistar-Kyoto rat (WKY) spontaneously hypertensive (SHR), an animal model human essential EVs were isolated from aortic WKY (WKY-EVs) SHR (SHR-EVs). Compared with WKY-EVs,...
Media-to-intima migration of vascular smooth muscle cells (VSMCs) is critical to intimal thickening in atherosclerosis and restenosis after coronary angioplasty. The aim this study determine the effects salusin-β on VSMC hyperplasia injury underlying mechanism.In vitro, promoted migration, which was attenuated by matrix metalloproteinase (MMP)-9 inhibition. Inhibition or knockdown p65-nuclear factor kappa beta (NFκB) VSMCs suppressed salusin-β-induced MMP-9 expression migration. Salusin-β...
Migration of vascular smooth muscle cells (VSMCs) is pivotal for remodeling in hypertension. Vascular adventitial fibroblasts (AFs) are important the homeostasis structure. This study designed to investigate roles AF exosomes (AFE) VSMC migration and underling mechanism. Primary VSMCs AFs were obtained from aorta spontaneously hypertensive rats (SHR) Wistar-Kyoto (WKY) rats. was evaluated with Boyden chamber assay wound healing assay. AFE WKY SHR isolated identified. promoted but had no...
Neutrophil (PMN) mobilization to sites of insult is critical for host defense and requires transendothelial migration (TEM). TEM involves several well-studied sequential adhesive interactions with vascular endothelial cells (ECs); however, what initiates or terminates this process not well-understood. Here we describe believe be a new mechanism where vessel associated macrophages (VAMs) through localized primed EC responses form ICAM-1 "hot spots", support PMN TEM. Using real-time intravital...
Inflammatory Bowel Disease (IBD) features en masse neutrophil (PMN) infiltration of the colon tissue, where PMNs occupy spatially distinct niches, including lamina propria mucosa (LPNs) and crypt epithelium (epithelium-associated neutrophils or EANs). Spatial PMN localization is currently used as a clinical disease scoring parameter, EAN presence has been correlated with severity prognosis reduced response to therapy. Surprisingly, although heterogeneity their relevance in IBD now...
Vascular smooth muscle cells (VSMCs) are indispensible components in foam cell formation. Salusin-β is a stimulator the progression of atherosclerosis. Here, we showed that salusin-β increased formation evidenced by accumulation lipid droplets and intracellular cholesterol content, promoted monocyte adhesion human VSMCs. expressions activity acyl coenzyme A:cholesterol acyltransferase-1 (ACAT-1) vascular molecule-1 (VCAM-1) Silencing ACAT-1 abolished salusin-β-induced accumulation, silencing...
Objective: Phenotypic transformation of adventitial fibroblasts is important in the pathogenesis hypertension. This study was designed to determine whether fibronectin type III domain containing 5 (FNDC5) alleviates phenotypic hypertension and underlying mechanisms. Methods results: Experiments were carried out spontaneously hypertensive rats (SHR) Wistar–Kyoto (WKY) primary aortic fibroblasts. FNDC5 downregulated NLRP3 inflammasome activated adventitia SHR. overexpression attenuated...
Neutrophil (PMN) recruitment to sites of insult is critical for host defense, however excessive PMN activity and tissue accumulation can lead exacerbated inflammation injury. Myeloperoxidase (MPO) a azurophilic granule enzyme, which together with H 2 O , forms powerful antimicrobial system designed kill ingested bacteria. Intriguingly, in addition intracellular killing invading microorganisms extracellular damage due generation ROS, soluble MPO has been directly implicated modulating...
Abstract Chemical stimulation of white adipose tissue (WAT) causes afferent reflex (AAR) and sympathetic activation. This study is to investigate the effects AAR on lipolysis mechanisms attenuated response enhanced in obesity. Obesity was caused by high-fat diet for 12 weeks rats. induced injection capsaicin into inguinal WAT or electrical epididymal nerve. activation, which obesity increased cAMP levels PKA activity, promoted hormone sensitive lipase (HSL) perilipin phosphorylation WAT,...
Abstract Salusin-β is a bioactive peptide involved in vascular smooth muscle cell proliferation, fibrosis and hypertension. The present study was designed to determine the effects of silencing salusin-β on hypertension cardiovascular remodeling spontaneously hypertensive rats (SHR). Thirteen-week-old male SHR normotensive Wistar-Kyoto (WKY) were subjected intravenous injection PBS, adenoviral vectors encoding shRNA (Ad-Sal-shRNA) or scramble shRNA. levels plasma, myocardium mesenteric artery...
Cardiac sympathetic afferent reflex (CSAR) enhancement contributes to exaggerated activation in chronic heart failure (CHF). The current study aimed investigate the roles of angiotensin (Ang)-(1-7) CSAR modulation and Ang-(1-7) signaling pathway paraventricular nucleus CHF rats.CHF was induced by coronary artery ligation. Responses renal nerve activity (RSNA) mean arterial pressure (MAP) epicardial application capsaicin were used evaluate rats with anesthesia.Ang-(1-7) increased RSNA, MAP,...
Ulcerative colitis (UC) is a chronic relapsing disease featuring aberrant accumulation of neutrophils in colonic mucosa and the luminal space. Although significant advances UC therapy have been made with development novel biologics small molecules targeting immune responses, success most current therapies still limited, safety concerns. Thus, there need to develop additional safe effective for treatment UC. Antimalarial drugs safely used many years resolve tissue inflammation associated...
Neutrophil (PMN) tissue accumulation is an established feature of ulcerative colitis (UC) lesions and colorectal cancer (CRC). Given the emerging evidence PMN phenotypic functional heterogeneity, we analyzed transcriptomic landscape blood PMNs during inflammatory ulceration to CRC transition. Transcriptional programs effectively separated based on their localization peripheral blood, inflamed colon tumor tissue. In silico pathway overrepresentation analysis, protein-network mapping, gene...
Abstract Macrophages, play important roles in initiation and resolution of inflammation. Notably, a subset tissue macrophages strategically localizes to the blood vessels, exerting essential regulatory functions at blood-tissue interface, including regulation vascular permeability neutrophil (PMN) transendothelial migration (TEM). Although number vessel-associated (VAMs) is increased inflamed tissue, underlying mechanisms VAMs recruitment are unknown. Our current study uncovers novel...
Rapid neutrophil (PMN) mobilization to sites of insult is critical for host defense and requires crossing the vascular wall. PMN transendothelial migration (TEM) involves several well-studied sequential adhesive interactions with ECs, however what initiates or terminates this process not well-understood. Our findings identified a new mechanism where gut interstitial macrophages, which are rapidly recruited towards wall in response inflammatory cues, were found locally prime endothelial cells...