A5100364082- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Microfluidic and Bio-sensing Technologies
- Molecular Sensors and Ion Detection
- Anodic Oxide Films and Nanostructures
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Diabetic Foot Ulcer Assessment and Management
- Advanced Proteomics Techniques and Applications
- Cholinesterase and Neurodegenerative Diseases
- Peptidase Inhibition and Analysis
- Multiple Myeloma Research and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Sulfur Compounds in Biology
- Cardiac Fibrosis and Remodeling
- Nanopore and Nanochannel Transport Studies
- Cancer, Lipids, and Metabolism
- Digital Imaging for Blood Diseases
- RNA regulation and disease
- Innovative Microfluidic and Catalytic Techniques Innovation
- Gold and Silver Nanoparticles Synthesis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Nanoplatforms for cancer theranostics
Second Affiliated Hospital of Xi'an Jiaotong University
2018-2025
Minhang District Central Hospital
2024
Fudan University
2011-2024
Shandong University of Technology
2023-2024
Renji Hospital
2021-2024
Shanghai Jiao Tong University
2021-2024
Chinese PLA General Hospital
2021-2024
China Astronaut Research and Training Center
2023-2024
Weifang Medical University
2023
Yangtze University
2023
Exosomes are cell-derived nano-sized vesicles that have been recently recognized as new mediators for many cellular processes and potential biomarkers non-invasive disease diagnosis the monitoring of treatment response. To better elucidate biology clinical value exosomes, there is a pressing need analytical technologies capable efficient isolation sensitive analysis such small molecularly diverse vesicles. Herein, we developed microfluidic exosome platform based on graphene...
Selective enrichment of phosphoproteins or phosphopeptides from complex mixtures is essential for MS-based phosphoproteomics, but still remains a challenge. In this article, we described an unprecedented approach to synthesize magnetic mesoporous Fe(3)O(4)@mTiO(2) microspheres with well-defined core/shell structure, pure and highly crystalline TiO(2) layer, high specific surface area (167.1 m(2)/g), large pore volume (0.45 cm(3)/g), appropriate tunable size (8.6-16.4 nm), susceptibility. We...
An integrated, 3D nano-engineered exosome profiling platform (ExoProfile chip) was developed to afford ultrasensitive, multiplexed detection of a panel surface protein markers on circulating exosomes with minuscular volume plasma.
An inkjet-printed nanochip integrating multiparametric extracellular vesicle analyses monitors tumor burden via liquid biopsy.
The clinical potential of miRNA-based liquid biopsy has been largely limited by the heterogeneous sources in plasma and tedious assay processes. Here, we develop a precise robust one-pot called dual-surface-protein-guided orthogonal recognition tumor-derived exosomes situ profiling microRNAs (SORTER) to detect exosomal miRNAs enhance diagnostic accuracy prostate cancer (PCa). SORTER uses two allosteric aptamers against marker CD63 tumor EpCAM create an labeling barcode achieve selective...
Tumor-derived extracellular vesicles (T-EVs) represent valuable markers for tumor diagnosis and treatment guidance. However, nanoscale sizes the low abundance of marker proteins T-EVs restrict interfacial affinity reaction, leading to isolation efficiency detection sensitivity. Here, we engineer a fluid nanoporous microinterface (FluidporeFace) in microfluidic chip by decorating supported lipid bilayers (SLBs) on herringbone microstructures with multiscale-enhanced reaction efficient T-EVs....
The protein phenotypes of extracellular vesicles (EVs) have emerged as promising biomarkers for cancer diagnosis and treatment monitoring. However, the technical challenges in rapid isolation multiplexed molecular detection EVs limited their clinical practice. Herein, we developed a magnetically driven tandem chip to achieve streamlined profiling surface EVs. Driven by magnetic force, nanomixers not only act tiny stir bars promote mass transfer enhance reaction efficiency EVs, but also...
The capture and detection of circulating tumor cells (CTCs) in the bloodstream patients with cancer is crucial for clinical diagnosis therapy. In present work, a facile integrated approach based on novel nitrocellulose membrane substrate large-scale surface-enhanced Raman scattering (SERS) imaging technology has been developed CTCs' sensitive enumeration. system mainly consists three aspects: CTCs bloodstream, SERS probes labeling captured readout NC was used to prepare CTC-capture through...
A microwell-patterned microfluidic digital mRNA analysis platform enables PCR-free, single-molecule detection of EWS-FLI1 fusion transcripts in EVs towards liquid biopsy-based non-invasive diagnosis Ewing Sarcoma.
The diagnostic criteria for cM0 (i+) stage proposed by American Joint Committee on Cancer (AJCC) in renal cell carcinoma (RCC) still remains unclear. present study aimed to establish and validate the of based postoperative circulating tumor cells (CTCs) monitoring patients with localized (LRCC). This enrolled 204 LRCC who received partial or radical nephrectomy from January 2015 November 2021. Cases were randomly divided into test set validation set. correlation between clinicopathological...
MicroRNAs (miRNAs) in tumor-derived extracellular vesicles (tEVs) are important cancer biomarkers for screening and early diagnosis. Multiplex detection of miRNAs tEVs facilitates accurate diagnosis but remains a challenge. Herein, we propose an encoded fusion strategy to profile the miRNA signature pancreatic A panel encoded-targeted-fusion beads was fabricated selective recognition tEVs, with turn-on fluorescence signals molecule beacons quantification barcode identification using readily...
Background: Circulating tumor cells (CTCs) and relevant autophagy Beclin-1 genes expression are critical biomarkers for tumorigenesis progress. Here we investigated the relationship of dynamic changes CTCs with renal cell carcinoma (RCC) prognosis.Materials methods: A total 69 patients RCC were enrolled divided into two groups based on postoperative status distant metastasis, including metastasis-free group (n = 58) metastatic 11). Demographic characteristics each patient recorded in detail....
A robust microfluidic alternating-pull–push active digitization method based on pneumatic valve actuation enables reproducible and highly efficient of small-volume samples for dPCR.
The identification of new biomarkers for the early detection hepatocellular carcinoma is critical in development tumor-targeted therapy, which possibly advantageous on prognosis this disease. Results from our previous study indicated that CCL15 can be a specific proteomic biomarker carcinoma, plays an important role tumorigenesis and tumor invasion. In study, we found induce cell migration Furthermore, CCR1, receptor CCL15, was demonstrated to play metastatic carcinoma. CCR1 short hairpin...
Background Nicotinamide adenine dinucleotide (NAD+) plays a key role in neuroinflammation and neurodegeneration provides anti-inflammatory neuroprotective effects multiple sclerosis (MS) experimental autoimmune encephalomyelitis (EAE).
Abstract Trauma is a significant health issue that not only leads to immediate death in many cases but also causes severe complications, such as sepsis, thrombosis, haemorrhage, acute respiratory distress syndrome and traumatic brain injury, among trauma patients. Target protein identification technology vital technique the field of biomedical research, enabling study biomolecular interactions, drug discovery disease treatment. It plays crucial role identifying key targets associated with...