A5002632947- Gut microbiota and health
- Probiotics and Fermented Foods
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Herpesvirus Infections and Treatments
- 3D Printing in Biomedical Research
- Metabolomics and Mass Spectrometry Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Peptidase Inhibition and Analysis
- Research, Science, and Academia
- Critical Race Theory in Education
- Iron oxide chemistry and applications
- Radiopharmaceutical Chemistry and Applications
- Race, History, and American Society
- Cell Image Analysis Techniques
- Animal Genetics and Reproduction
- Electronic and Structural Properties of Oxides
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Gold and Silver Nanoparticles Synthesis and Applications
- Fusion materials and technologies
- Retinal Development and Disorders
- Machine Learning in Bioinformatics
- Barrier Structure and Function Studies
- Fungal and yeast genetics research
Oak Ridge National Laboratory
1998-2021
Oak Ridge Associated Universities
2020
University of Virginia
2014
Argonne National Laboratory
1999
Uniformed Services University of the Health Sciences
1994
University of Tennessee at Knoxville
1991-1994
The formation and composition of the protein corona on silica (SiO2) nanoparticles (NP) with different surface chemistries was evaluated over time. Native SiO2, amine (–NH2) carboxy (–COO−) modified NP were examined following incubation in mammalian growth media containing fetal bovine serum (FBS) for 1, 4, 24 48 hours. transition from its early dynamic state to later more stable using mass spectrometry. diameter 22.4 ± 2.2 nm measured by scanning transmission electron microscopy (STEM)....
Light chain (AL) amyloidosis is the most common form of systemic amyloid disease, and cardiomyopathy a dire consequence, resulting in an extremely poor prognosis. AL characterized by production monoclonal free light chains that deposit as fibrils principally heart, liver, kidneys causing organ dysfunction. We have studied effects fibrils, produced from recombinant λ6 variable domains, on metabolic activity human cardiomyocytes. The data indicate at 0.1 μM, but not monomer, significantly...
Cell‐free protein synthesis (CFPS) is a powerful technology that allows for optimization of production without maintenance living system. Integrated within micro and nanofluidic architectures, CFPS can be optimized point‐of‐care use. Here, the development microfluidic bioreactor designed to facilitate single‐dose therapeutic protein, in small footprint device at point‐of‐care, described. This new design builds on use long, serpentine channel enhanced by integrating nanofabricated membrane...
Abstract Predicting the range of substrates accepted by an enzyme from its amino acid sequence is challenging. Although sequence‐ and structure‐based annotation approaches are often accurate for predicting broad categories substrate specificity, they generally cannot predict which specific molecules will be as a given enzyme, particularly within class closely related molecules. Combining targeted experimental activity data with structural modeling, ligand docking, physicochemical properties...
The microbiome influences health and disease through complex networks of host genetics, genomics, microbes, environment. Identifying the mechanisms these interactions has remained challenging. Systems genetics in laboratory mice (
Cell-free protein synthesis (CFPS) has the potential to produce enzymes, therapeutic agents, and other proteins, while circumventing difficulties associated with in vivo heterologous expression. However, contents of cell-free extracts used carry out are generally not characterized, which hampers progress toward enhancing yield or functional activity target protein. We explored utility mass spectrometry (MS)-based proteomics for characterizing bacterial transcribing translating gene sequences...
Abstract Background Analysis of an allelic series point mutations in a gene, generated by N -ethyl- -nitrosourea (ENU) mutagenesis, is valuable method for discovering the full scope its biological function. Here we present efficient gene-driven approach identifying ENU-induced any gene C57BL/6J mice. The advantage such that it allows one to select interest mouse genome and go directly from DNA sequence mutant Results We produced Cryopreserved Mutant Mouse Bank (CMMB), which archive DNA,...
A new synthesis approach is described that allows the direct incorporation of fluorescent labels into volume or body SiO2 nanoparticles. In this process, Alexa Fluor dyes with different emission wavelengths were covalently incorporated nanoparticles during their formation by hydrolysis tetraethoxysilane. The dye molecules homogeneously distributed throughout quantum yields volume-labeled much higher than labeled using conventional organic dyes. size resulting was controlled microemulsion...
Protein based therapeutics are an important class of drugs, used to treat a variety medical conditions including cancer and autoimmune diseases. Requiring continuous cold storage, having limited shelf life, the ability produce such at point-of-care would open up new opportunities in distributing medicines treating patients more remote locations. Here, authors describe first steps development microfluidic platform that can be for protein synthesis. While biologic medicines, therapeutic...
La(<sup>227</sup>Th)PO<sub>4</sub>core +2 shells nanoparticles retained >99.75% of activity from<sup>227</sup>Th and decay daughters (<sup>223</sup>Ra,<sup>211</sup>Pb) for targeted alpha therapy.
Multiscale fluidic reactors with integrated nanoporous membranes enable prolonged cell-free reactions for efficient therapeutic protein production as required point-of-care needs. S. T. Retterer and co-workers show on page 810 how the membrane permeability can be tuned to optimize exchange between a primary reaction channel, containing machinery, 'feeder' channel that supplies raw materials energy system. Improvements in both yield rates compared commercial systems are realized.
Abstract The role of the microbiome in health and disease involves complex networks host genetics, genomics, microbes environment. Identifying mechanisms these interactions has remained challenging. Systems genetics laboratory mouse enables data-driven discovery network components host-microbial underlying multiple phenotypes. To examine interplay among whole genome, transcriptome microbiome, we mapped quantitative trait loci correlated abundance cecal mRNA, luminal microflora, physiology...
The microvasculature system is critical for the delivery and removal of key nutrients waste products significantly damaged by ionizing radiation. Single-cell capillaries structures are primary cause circulatory dysfunction, one that results in morbidities leading to progressive tissue organ failure premature death. Identifying tissue-specific biomarkers predictive extent damage will aid developing medical countermeasures treating individuals exposed In this pilot study, we developed tested a...
In this paper, we study segmentation of tight junctions and analyze the formation integrity in large-scale confocal image stacks, a challenging biological problem because low spatial resolution images presence breaks junction structure. We present an automated, three-step processing approach for analysis. our approach, first localize each individual nucleus by using thresholding, morphological filters active contours. By position as seed point, automatically segment cell body based on...
Predicting the range of substrates accepted by an enzyme from its amino acid sequence is challenging. Although sequence- and structure-based annotation approaches are often accurate for predicting broad categories substrate specificity, they generally cannot predict which specific molecules will be as a given enzyme, particularly within class closely related molecules. Combining targeted experimental activity data with structural modeling, ligand docking, physicochemical properties proteins...
Physiologically-relevant in vitro models of retinal disease are necessary for understanding the complex interactions oxidative stress, molecular signaling and physical contact between cells their local environment. In this study, microfluidic devices microcontact printing used to mimic vivo conditions sub-retinal microenvironment effects stress atrophy on protein expression by pigment epithelial cells. The results demonstrate that differences RNA due loss function can be observed from within...