A5061647396- Glioma Diagnosis and Treatment
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Cancer therapeutics and mechanisms
- RNA modifications and cancer
- ATP Synthase and ATPases Research
- Nanoparticle-Based Drug Delivery
- Ultrasound and Hyperthermia Applications
- Cancer-related molecular mechanisms research
- Photoacoustic and Ultrasonic Imaging
- Semiconductor materials and devices
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Cancer Treatment and Pharmacology
- Plant Molecular Biology Research
- Synthesis and Catalytic Reactions
- 14-3-3 protein interactions
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Ion channel regulation and function
- Genetic Syndromes and Imprinting
- Cancer Cells and Metastasis
- Genetics and Neurodevelopmental Disorders
- Advanced Electron Microscopy Techniques and Applications
Universidad Nacional Autónoma de México
2010-2024
University of Miami
2024
Howard Hughes Medical Institute
2022-2024
Sylvester Comprehensive Cancer Center
2024
NYU Langone Health
2024
Northwestern University
2018-2021
Northwestern Medicine
2021
Neurological Surgery
2019-2020
Columbia University
2017
The multifunctional CCCTC-binding factor (CTCF) protein exhibits a broad range of functions, including that insulator and higher-order chromatin organizer. We found CTCF comprises previously unrecognized region is necessary sufficient to bind RNA (RNA-binding [RBR]) distinct from its DNA-binding domain. Depletion cellular led decrease in not only levels p53 mRNA, as expected, but also those Wrap53 RNA, an antisense transcript originated the locus. PAR-CLIP-seq (photoactivatable...
Paclitaxel shows little benefit in the treatment of glioma due to poor penetration across blood-brain barrier (BBB). Low-intensity pulsed ultrasound (LIPU) with microbubble injection transiently disrupts BBB allowing for improved drug delivery brain. We investigated distribution, toxicity, and efficacy LIPU two different formulations paclitaxel, albumin-bound paclitaxel (ABX) dissolved cremophor (CrEL-PTX), preclinical models.The biodistribution ABX CrEL-PTX were compared without delivery....
Abstract CCCTC-binding factor (CTCF) is critical to three-dimensional genome organization. Upon differentiation, CTCF insulates active and repressed genes within Hox gene clusters. We conducted a genome-wide CRISPR knockout (KO) screen identify required for CTCF-boundary activity at the HoxA cluster, complemented by biochemical approaches. Among candidates, we identified Myc-associated zinc-finger protein (MAZ) as cofactor in insulation. MAZ colocalizes with chromatin borders and, similar...
Partitioning of repressive from actively transcribed chromatin in mammalian cells fosters cell-type-specific gene expression patterns. While this partitioning is reconstructed during differentiation, the occupancy key insulator, CCCTC-binding factor (CTCF), unchanged at developmentally important Hox clusters. Thus, dynamic changes boundaries must entail other activities. Given its requirement for loop formation, we examined cohesin-based without known insulators, CTCF and Myc-associated...
Post-transcriptional regulation by microRNAs is recognized as one of the major pathways for control cellular homeostasis. Less well understood transcriptional and epigenetic genes encoding microRNAs. In present study we addressed miR-181c in normal malignant brain cells. To explore evaluated its expression using RT-qPCR vivo binding CCCTC-binding factor (CTCF) to regulatory region different glioblastoma cell lines. DNA methylation survey, chromatin immunoprecipitation RNA interference assays...
Abstract Background Long-term gene silencing throughout cell division is generally achieved by DNA methylation and other epigenetic processes. Aberrant now widely recognized to be associated with cancer human diseases. Here we addressed the contribution of multifunctional nuclear factor CTCF regulation retinoblastoma ( Rb ) promoter in different tumoral lines. Methods To assess status promoter, genomic from stably transfected erythroleukemic K562 cells expressing a GFP reporter transgene was...
Genome organization into transcriptionally active domains denotes one of the first levels gene expression regulation. Although chromatin domain concept is generally accepted, only little known on how impacts regulation differential expression. Insulators might hold answers to address this issue as they delimit and organize domains. We have previously identified a CTCF-dependent insulator with enhancer-blocking activity embedded in 5' non-coding region chicken α-globin domain. Here, we...
Glioblastoma (GBM) is one of the most aggressive primary brain tumors with frequent recurrences following standard methods treatment-temozolomide (TMZ), ionizing radiation and surgical resection. The objective our study was to investigate GBM resistance mediated via MMP14 (matrix metalloproteinase 14). We used multiple PDX models established glioma cell lines characterize expression subcellular localization after TMZ treatment. performed a Kiloplex ELISA-based array evaluate changes in...
SUMMARY Partitioning of repressive from actively transcribed chromatin in mammalian cells fosters cell-type specific gene expression patterns. While this partitioning is reconstructed during differentiation, the occupancy key insulator, CTCF, unchanged at developmentally important Hox clusters. Thus, dynamic changes boundaries must entail other activities. Given its requirement for loop formation, we examined cohesin-based without known insulators, CTCF and MAZ, identified a family zinc...
-regulatory elements (CREs) play crucial roles in regulating gene expression during erythroid cell differentiation. Genome-wide erythroid-specific CREs have not been characterized chicken cells, which is an organism model used to study epigenetic regulation erythropoiesis.
The epigenetic mechanisms involved in transcriptional regulation leading to malignant phenotype gliomas remains poorly understood. Topoisomerase IIB (TOP2B), an enzyme that decoils and releases torsional forces DNA, is overexpressed a subset of gliomas. Therefore, we investigated its role these tumors.
<p>Figure S1-S11</p>
<div>AbstractPurpose:<p>The epigenetic mechanisms involved in transcriptional regulation leading to malignant phenotype gliomas remains poorly understood. Topoisomerase IIB (TOP2B), an enzyme that decoils and releases torsional forces DNA, is overexpressed a subset of gliomas. Therefore, we investigated its role these tumors.</p>Experimental Design:<p>To investigate the TOP2B gliomas, performed paired chromatin immunoprecipitation sequencing for RNA-sequencing...
<div>AbstractPurpose:<p>Paclitaxel shows little benefit in the treatment of glioma due to poor penetration across blood–brain barrier (BBB). Low-intensity pulsed ultrasound (LIPU) with microbubble injection transiently disrupts BBB allowing for improved drug delivery brain. We investigated distribution, toxicity, and efficacy LIPU two different formulations paclitaxel, albumin-bound paclitaxel (ABX) dissolved cremophor (CrEL-PTX), preclinical models.</p>Experimental...
Abstract Topoisomerase II poisons are one of the most common class chemotherapeutics used in cancer. We show that glioblastoma (GBM), malignant all primary brain tumors adults is responsive to TOP2 poisons. To identify genes confer susceptibility this drug gliomas, we performed a genome-scale CRISPR knockout screen with etoposide. Genes involved protein synthesis and DNA damage were implicated etoposide susceptibility. define potential biomarkers for poisons, hits overlapped whose expression...
Abstract Cancer immunoediting shapes tumor progression by the selection of cell variants that can evade immune recognition. Given evasion and intra-tumor heterogeneity intrinsic to gliomas, we hypothesized CD8+ T-cells mediate in these tumors. We evaluated glioma absence depleting this population transgenic murine gliomas. Upon transplantation, gliomas developed engrafted poorly recipients with intact immunity but well those T-cell depletion. Gliomas exhibited increased chromosomal...
<p>Supplementary Methods and Materials Figures S1: Schematic representation of LIPU generator set up. Supp Table 1: Summary histological evaluation CNS after one course US-PTX Supp. 2: STR results PDX cell lines. S2: Cell viability in response to PTX ABX</p>
<div>AbstractPurpose:<p>Paclitaxel shows little benefit in the treatment of glioma due to poor penetration across blood–brain barrier (BBB). Low-intensity pulsed ultrasound (LIPU) with microbubble injection transiently disrupts BBB allowing for improved drug delivery brain. We investigated distribution, toxicity, and efficacy LIPU two different formulations paclitaxel, albumin-bound paclitaxel (ABX) dissolved cremophor (CrEL-PTX), preclinical models.</p>Experimental...